Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_3376

Experiment: L-Glutamine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_3374 and Psest_3375 overlap by 4 nucleotidesPsest_3375 and Psest_3376 are separated by 53 nucleotidesPsest_3376 and Psest_3377 are separated by 119 nucleotidesPsest_3377 and Psest_3378 overlap by 8 nucleotides Psest_3374: Psest_3374 - Tfp pilus assembly protein, tip-associated adhesin PilY1, at 3,541,786 to 3,545,556 _3374 Psest_3375: Psest_3375 - prepilin-type N-terminal cleavage/methylation domain, at 3,545,553 to 3,545,999 _3375 Psest_3376: Psest_3376 - (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate reductase (IPP and DMAPP forming), at 3,546,053 to 3,546,997 _3376 Psest_3377: Psest_3377 - FKBP-type peptidyl-prolyl cis-trans isomerases 2, at 3,547,117 to 3,547,554 _3377 Psest_3378: Psest_3378 - lipoprotein signal peptidase, at 3,547,547 to 3,548,050 _3378 Position (kb) 3546 3547Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3545.275 kb on + strandat 3545.317 kb on + strandat 3545.404 kb on - strandat 3545.670 kb on + strand, within Psest_3375at 3545.670 kb on + strand, within Psest_3375at 3545.670 kb on + strand, within Psest_3375at 3545.670 kb on + strand, within Psest_3375at 3545.670 kb on + strand, within Psest_3375at 3545.670 kb on + strand, within Psest_3375at 3545.670 kb on + strand, within Psest_3375at 3545.670 kb on + strand, within Psest_3375at 3545.671 kb on - strand, within Psest_3375at 3545.671 kb on - strand, within Psest_3375at 3545.742 kb on + strand, within Psest_3375at 3545.742 kb on + strand, within Psest_3375at 3545.742 kb on + strand, within Psest_3375at 3545.742 kb on + strand, within Psest_3375at 3545.760 kb on + strand, within Psest_3375at 3545.760 kb on + strand, within Psest_3375at 3545.760 kb on + strand, within Psest_3375at 3545.760 kb on + strand, within Psest_3375at 3545.764 kb on + strand, within Psest_3375at 3545.764 kb on + strand, within Psest_3375at 3545.832 kb on + strand, within Psest_3375at 3545.832 kb on + strand, within Psest_3375at 3545.833 kb on - strand, within Psest_3375at 3545.870 kb on + strand, within Psest_3375at 3545.870 kb on + strand, within Psest_3375at 3545.870 kb on + strand, within Psest_3375at 3545.870 kb on + strand, within Psest_3375at 3545.870 kb on + strand, within Psest_3375at 3545.870 kb on + strand, within Psest_3375at 3545.870 kb on + strand, within Psest_3375at 3545.871 kb on - strand, within Psest_3375at 3545.871 kb on - strand, within Psest_3375at 3545.871 kb on - strand, within Psest_3375at 3545.874 kb on + strand, within Psest_3375at 3545.875 kb on - strand, within Psest_3375at 3545.875 kb on - strand, within Psest_3375at 3545.875 kb on - strand, within Psest_3375at 3545.875 kb on - strand, within Psest_3375at 3545.875 kb on - strand, within Psest_3375at 3547.038 kb on + strandat 3547.038 kb on + strandat 3547.397 kb on + strand, within Psest_3377

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamine (C)
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3,545,275 + -0.0
3,545,317 + +0.2
3,545,404 - +1.1
3,545,670 + Psest_3375 0.26 +0.7
3,545,670 + Psest_3375 0.26 +0.9
3,545,670 + Psest_3375 0.26 -0.1
3,545,670 + Psest_3375 0.26 +0.0
3,545,670 + Psest_3375 0.26 -1.5
3,545,670 + Psest_3375 0.26 +2.7
3,545,670 + Psest_3375 0.26 +1.1
3,545,670 + Psest_3375 0.26 -0.7
3,545,671 - Psest_3375 0.26 -1.1
3,545,671 - Psest_3375 0.26 +0.6
3,545,742 + Psest_3375 0.42 -1.6
3,545,742 + Psest_3375 0.42 -0.7
3,545,742 + Psest_3375 0.42 +1.0
3,545,742 + Psest_3375 0.42 -1.8
3,545,760 + Psest_3375 0.46 +0.2
3,545,760 + Psest_3375 0.46 +0.8
3,545,760 + Psest_3375 0.46 +0.6
3,545,760 + Psest_3375 0.46 +0.3
3,545,764 + Psest_3375 0.47 -0.9
3,545,764 + Psest_3375 0.47 +0.1
3,545,832 + Psest_3375 0.62 +0.3
3,545,832 + Psest_3375 0.62 -1.0
3,545,833 - Psest_3375 0.63 +0.1
3,545,870 + Psest_3375 0.71 -0.2
3,545,870 + Psest_3375 0.71 -0.6
3,545,870 + Psest_3375 0.71 +0.1
3,545,870 + Psest_3375 0.71 -0.8
3,545,870 + Psest_3375 0.71 -0.7
3,545,870 + Psest_3375 0.71 +0.5
3,545,870 + Psest_3375 0.71 +0.3
3,545,871 - Psest_3375 0.71 -2.8
3,545,871 - Psest_3375 0.71 -2.5
3,545,871 - Psest_3375 0.71 -0.2
3,545,874 + Psest_3375 0.72 -0.2
3,545,875 - Psest_3375 0.72 -1.6
3,545,875 - Psest_3375 0.72 -0.8
3,545,875 - Psest_3375 0.72 -1.6
3,545,875 - Psest_3375 0.72 -1.1
3,545,875 - Psest_3375 0.72 +1.4
3,547,038 + +0.5
3,547,038 + +1.0
3,547,397 + Psest_3377 0.64 -0.5

Or see this region's nucleotide sequence