Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_2017

Experiment: L-Glutamine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_2015 and Psest_2016 overlap by 4 nucleotidesPsest_2016 and Psest_2017 are separated by 6 nucleotidesPsest_2017 and Psest_2018 overlap by 8 nucleotides Psest_2015: Psest_2015 - Type II secretory pathway, component PulD, at 2,134,961 to 2,135,770 _2015 Psest_2016: Psest_2016 - hypothetical protein, at 2,135,767 to 2,136,396 _2016 Psest_2017: Psest_2017 - Predicted acyltransferase, at 2,136,403 to 2,136,828 _2017 Psest_2018: Psest_2018 - Uncharacterized conserved protein, at 2,136,821 to 2,137,987 _2018 Position (kb) 2136 2137Strain fitness (log2 ratio) -1 0 1 2at 2135.616 kb on + strand, within Psest_2015at 2135.646 kb on - strand, within Psest_2015at 2135.717 kb on + strandat 2135.717 kb on + strandat 2135.718 kb on - strandat 2135.758 kb on + strandat 2135.841 kb on + strand, within Psest_2016at 2135.842 kb on - strand, within Psest_2016at 2135.864 kb on + strand, within Psest_2016at 2135.865 kb on - strand, within Psest_2016at 2135.865 kb on - strand, within Psest_2016at 2135.865 kb on - strand, within Psest_2016at 2135.865 kb on - strand, within Psest_2016at 2135.865 kb on - strand, within Psest_2016at 2135.873 kb on + strand, within Psest_2016at 2135.908 kb on + strand, within Psest_2016at 2135.934 kb on - strand, within Psest_2016at 2135.976 kb on + strand, within Psest_2016at 2136.005 kb on - strand, within Psest_2016at 2136.005 kb on - strand, within Psest_2016at 2136.128 kb on + strand, within Psest_2016at 2136.128 kb on + strand, within Psest_2016at 2136.489 kb on - strand, within Psest_2017at 2136.489 kb on - strand, within Psest_2017at 2136.489 kb on - strand, within Psest_2017at 2136.533 kb on - strand, within Psest_2017at 2136.776 kb on - strand, within Psest_2017at 2136.776 kb on - strand, within Psest_2017at 2136.776 kb on - strand, within Psest_2017at 2136.822 kb on + strandat 2137.186 kb on + strand, within Psest_2018at 2137.186 kb on + strand, within Psest_2018at 2137.187 kb on - strand, within Psest_2018at 2137.365 kb on + strand, within Psest_2018at 2137.575 kb on + strand, within Psest_2018at 2137.575 kb on + strand, within Psest_2018at 2137.576 kb on - strand, within Psest_2018at 2137.617 kb on + strand, within Psest_2018at 2137.618 kb on - strand, within Psest_2018

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamine (C)
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2,135,616 + Psest_2015 0.81 -1.7
2,135,646 - Psest_2015 0.85 +1.0
2,135,717 + -1.0
2,135,717 + -0.7
2,135,718 - +0.2
2,135,758 + +0.2
2,135,841 + Psest_2016 0.12 -0.2
2,135,842 - Psest_2016 0.12 +0.6
2,135,864 + Psest_2016 0.15 -1.0
2,135,865 - Psest_2016 0.16 +0.7
2,135,865 - Psest_2016 0.16 -0.5
2,135,865 - Psest_2016 0.16 -0.6
2,135,865 - Psest_2016 0.16 +0.2
2,135,865 - Psest_2016 0.16 -0.2
2,135,873 + Psest_2016 0.17 +0.2
2,135,908 + Psest_2016 0.22 -0.2
2,135,934 - Psest_2016 0.27 -0.5
2,135,976 + Psest_2016 0.33 +0.5
2,136,005 - Psest_2016 0.38 -0.1
2,136,005 - Psest_2016 0.38 -1.7
2,136,128 + Psest_2016 0.57 +0.2
2,136,128 + Psest_2016 0.57 +0.4
2,136,489 - Psest_2017 0.20 +0.5
2,136,489 - Psest_2017 0.20 +1.6
2,136,489 - Psest_2017 0.20 +1.0
2,136,533 - Psest_2017 0.31 +0.5
2,136,776 - Psest_2017 0.88 +2.6
2,136,776 - Psest_2017 0.88 +0.8
2,136,776 - Psest_2017 0.88 +2.3
2,136,822 + +1.7
2,137,186 + Psest_2018 0.31 +1.2
2,137,186 + Psest_2018 0.31 +0.1
2,137,187 - Psest_2018 0.31 -0.1
2,137,365 + Psest_2018 0.47 +0.6
2,137,575 + Psest_2018 0.65 -0.1
2,137,575 + Psest_2018 0.65 -0.1
2,137,576 - Psest_2018 0.65 +0.5
2,137,617 + Psest_2018 0.68 +0.1
2,137,618 - Psest_2018 0.68 +0.9

Or see this region's nucleotide sequence