Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_2319

Experiment: L-tyrosine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_2318 and Psest_2319 are separated by 118 nucleotidesPsest_2319 and Psest_2320 are separated by 74 nucleotides Psest_2318: Psest_2318 - probable AcnD-accessory protein PrpF, at 2,470,047 to 2,471,234 _2318 Psest_2319: Psest_2319 - aconitate hydratase 1/2-methylisocitrate dehydratase, Fe/S-dependent, at 2,471,353 to 2,473,956 _2319 Psest_2320: Psest_2320 - 2-methylcitrate synthase/citrate synthase II, at 2,474,031 to 2,475,158 _2320 Position (kb) 2471 2472 2473 2474Strain fitness (log2 ratio) -2 -1 0 1 2at 2471.224 kb on - strandat 2471.315 kb on - strandat 2471.315 kb on - strandat 2471.315 kb on - strandat 2474.078 kb on - strandat 2474.078 kb on - strandat 2474.078 kb on - strandat 2474.078 kb on - strandat 2474.218 kb on + strand, within Psest_2320at 2474.288 kb on - strand, within Psest_2320at 2474.368 kb on + strand, within Psest_2320at 2474.368 kb on + strand, within Psest_2320at 2474.539 kb on + strand, within Psest_2320at 2474.753 kb on - strand, within Psest_2320at 2474.753 kb on - strand, within Psest_2320at 2474.753 kb on - strand, within Psest_2320at 2474.891 kb on + strand, within Psest_2320at 2474.953 kb on + strand, within Psest_2320at 2474.953 kb on + strand, within Psest_2320

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Per-strain Table

Position Strand Gene LocusTag Fraction L-tyrosine (C)
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2,471,224 - -1.6
2,471,315 - -0.1
2,471,315 - +0.2
2,471,315 - -0.6
2,474,078 - -0.6
2,474,078 - +2.4
2,474,078 - +0.6
2,474,078 - +0.3
2,474,218 + Psest_2320 0.17 -1.9
2,474,288 - Psest_2320 0.23 -1.2
2,474,368 + Psest_2320 0.30 -1.2
2,474,368 + Psest_2320 0.30 -0.0
2,474,539 + Psest_2320 0.45 +1.1
2,474,753 - Psest_2320 0.64 -0.9
2,474,753 - Psest_2320 0.64 +2.6
2,474,753 - Psest_2320 0.64 -0.1
2,474,891 + Psest_2320 0.76 +0.7
2,474,953 + Psest_2320 0.82 -1.6
2,474,953 + Psest_2320 0.82 +1.5

Or see this region's nucleotide sequence