Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_2304

Experiment: L-tyrosine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_2303 and Psest_2304 are separated by 140 nucleotidesPsest_2304 and Psest_2305 are separated by 6 nucleotidesPsest_2305 and Psest_2306 overlap by 4 nucleotides Psest_2303: Psest_2303 - glutaminyl-tRNA synthetase, at 2,453,444 to 2,455,174 _2303 Psest_2304: Psest_2304 - Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family, at 2,455,315 to 2,455,809 _2304 Psest_2305: Psest_2305 - UDP-2,3-diacylglucosamine hydrolase, at 2,455,816 to 2,456,538 _2305 Psest_2306: Psest_2306 - Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA, at 2,456,535 to 2,457,185 _2306 Position (kb) 2455 2456Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5 6at 2455.402 kb on + strand, within Psest_2304at 2456.536 kb on + strandat 2456.537 kb on - strandat 2456.659 kb on + strand, within Psest_2306at 2456.674 kb on + strand, within Psest_2306at 2456.674 kb on + strand, within Psest_2306at 2456.674 kb on + strand, within Psest_2306at 2456.675 kb on - strand, within Psest_2306at 2456.717 kb on - strand, within Psest_2306at 2456.727 kb on + strand, within Psest_2306at 2456.728 kb on - strand, within Psest_2306at 2456.728 kb on - strand, within Psest_2306

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Per-strain Table

Position Strand Gene LocusTag Fraction L-tyrosine (C)
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2,455,402 + Psest_2304 0.18 +6.5
2,456,536 + +2.8
2,456,537 - -0.8
2,456,659 + Psest_2306 0.19 +0.3
2,456,674 + Psest_2306 0.21 +3.3
2,456,674 + Psest_2306 0.21 +2.4
2,456,674 + Psest_2306 0.21 -0.9
2,456,675 - Psest_2306 0.22 -2.0
2,456,717 - Psest_2306 0.28 +1.2
2,456,727 + Psest_2306 0.29 -1.6
2,456,728 - Psest_2306 0.30 -1.1
2,456,728 - Psest_2306 0.30 +2.6

Or see this region's nucleotide sequence