Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0010

Experiment: L-tyrosine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0008 and Psest_0009 overlap by 4 nucleotidesPsest_0009 and Psest_0010 overlap by 32 nucleotidesPsest_0010 and Psest_0011 overlap by 4 nucleotides Psest_0008: Psest_0008 - 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 10,063 to 10,812 _0008 Psest_0009: Psest_0009 - histidinol-phosphate phosphatase family domain/HAD-superfamily hydrolase, subfamily IIIA, at 10,809 to 11,387 _0009 Psest_0010: Psest_0010 - glycyl-tRNA synthetase, tetrameric type, beta subunit, at 11,356 to 13,410 _0010 Psest_0011: Psest_0011 - glycyl-tRNA synthetase, tetrameric type, alpha subunit, at 13,407 to 14,354 _0011 Position (kb) 11 12 13 14Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 10.828 kb on - strandat 10.828 kb on - strandat 10.828 kb on - strandat 11.358 kb on - strandat 14.395 kb on - strandat 14.395 kb on - strandat 14.395 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-tyrosine (C)
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10,828 - +0.3
10,828 - +2.2
10,828 - -3.0
11,358 - -1.2
14,395 - +3.3
14,395 - +2.2
14,395 - +2.0

Or see this region's nucleotide sequence