Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3515 and Psest_3516 are separated by 128 nucleotides Psest_3516 and Psest_3517 are separated by 145 nucleotides
Psest_3515: Psest_3515 - Na+-dependent transporters of the SNF family, at 3,691,418 to 3,692,827
_3515
Psest_3516: Psest_3516 - Leucine dehydrogenase (EC 1.4.1.9) (from data) , at 3,692,956 to 3,694,050
_3516
Psest_3517: Psest_3517 - branched-chain ketoacid ferredoxin reductase (EC 1.2.7.7) active on 4-methyl-2-oxopentanoate, (S)-3-methyl-2-oxopentanoate, or 3-methyl-2-oxobutanoate (from data) , at 3,694,196 to 3,697,666
_3517
Position (kb)
3692
3693
3694
3695 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3692.037 kb on + strand, within Psest_3515 at 3692.038 kb on - strand, within Psest_3515 at 3692.190 kb on + strand, within Psest_3515 at 3692.190 kb on + strand, within Psest_3515 at 3692.190 kb on + strand, within Psest_3515 at 3692.415 kb on + strand, within Psest_3515 at 3692.415 kb on + strand, within Psest_3515 at 3692.415 kb on + strand, within Psest_3515 at 3692.415 kb on + strand, within Psest_3515 at 3692.415 kb on + strand, within Psest_3515 at 3692.415 kb on + strand, within Psest_3515 at 3692.415 kb on + strand, within Psest_3515 at 3692.415 kb on + strand, within Psest_3515 at 3692.416 kb on - strand, within Psest_3515 at 3692.416 kb on - strand, within Psest_3515 at 3692.416 kb on - strand, within Psest_3515 at 3692.416 kb on - strand, within Psest_3515 at 3692.445 kb on + strand, within Psest_3515 at 3692.445 kb on + strand, within Psest_3515 at 3692.446 kb on - strand, within Psest_3515 at 3692.628 kb on + strand, within Psest_3515 at 3692.628 kb on + strand, within Psest_3515 at 3692.629 kb on - strand, within Psest_3515 at 3692.629 kb on - strand, within Psest_3515 at 3692.629 kb on - strand, within Psest_3515 at 3692.629 kb on - strand, within Psest_3515 at 3692.670 kb on + strand, within Psest_3515 at 3692.687 kb on - strand at 3693.150 kb on - strand, within Psest_3516 at 3693.152 kb on + strand, within Psest_3516 at 3693.152 kb on + strand, within Psest_3516 at 3693.153 kb on - strand, within Psest_3516 at 3693.186 kb on - strand, within Psest_3516 at 3693.186 kb on - strand, within Psest_3516 at 3693.356 kb on + strand, within Psest_3516 at 3693.356 kb on + strand, within Psest_3516 at 3693.356 kb on + strand, within Psest_3516 at 3693.357 kb on - strand, within Psest_3516 at 3693.720 kb on + strand, within Psest_3516 at 3693.720 kb on + strand, within Psest_3516 at 3693.721 kb on - strand, within Psest_3516 at 3693.721 kb on - strand, within Psest_3516 at 3693.723 kb on - strand, within Psest_3516 at 3693.723 kb on - strand, within Psest_3516 at 3693.723 kb on - strand, within Psest_3516 at 3693.908 kb on + strand, within Psest_3516 at 3694.099 kb on + strand at 3694.100 kb on - strand at 3694.100 kb on - strand at 3694.100 kb on - strand at 3694.100 kb on - strand at 3694.362 kb on + strand at 3694.611 kb on + strand, within Psest_3517 at 3694.612 kb on - strand, within Psest_3517 at 3694.612 kb on - strand, within Psest_3517 at 3694.662 kb on + strand, within Psest_3517 at 3694.662 kb on + strand, within Psest_3517 at 3694.662 kb on + strand, within Psest_3517 at 3694.680 kb on + strand, within Psest_3517 at 3694.692 kb on + strand, within Psest_3517 at 3694.692 kb on + strand, within Psest_3517 at 3694.693 kb on - strand, within Psest_3517 at 3694.710 kb on + strand, within Psest_3517 at 3694.710 kb on + strand, within Psest_3517 at 3694.710 kb on + strand, within Psest_3517 at 3694.710 kb on + strand, within Psest_3517 at 3694.710 kb on + strand, within Psest_3517 at 3694.710 kb on + strand, within Psest_3517 at 3694.711 kb on - strand, within Psest_3517 at 3694.711 kb on - strand, within Psest_3517 at 3694.711 kb on - strand, within Psest_3517 at 3694.711 kb on - strand, within Psest_3517 at 3694.711 kb on - strand, within Psest_3517 at 3694.711 kb on - strand, within Psest_3517 at 3694.711 kb on - strand, within Psest_3517 at 3694.714 kb on - strand, within Psest_3517
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,692,037 + Psest_3515 0.44 -0.7 3,692,038 - Psest_3515 0.44 +0.3 3,692,190 + Psest_3515 0.55 +0.2 3,692,190 + Psest_3515 0.55 +0.1 3,692,190 + Psest_3515 0.55 +0.3 3,692,415 + Psest_3515 0.71 -1.4 3,692,415 + Psest_3515 0.71 -0.8 3,692,415 + Psest_3515 0.71 -0.2 3,692,415 + Psest_3515 0.71 -0.8 3,692,415 + Psest_3515 0.71 -0.1 3,692,415 + Psest_3515 0.71 -0.7 3,692,415 + Psest_3515 0.71 -0.5 3,692,415 + Psest_3515 0.71 -0.6 3,692,416 - Psest_3515 0.71 -0.2 3,692,416 - Psest_3515 0.71 -1.7 3,692,416 - Psest_3515 0.71 -0.5 3,692,416 - Psest_3515 0.71 -1.7 3,692,445 + Psest_3515 0.73 +0.1 3,692,445 + Psest_3515 0.73 +0.5 3,692,446 - Psest_3515 0.73 +1.1 3,692,628 + Psest_3515 0.86 -0.3 3,692,628 + Psest_3515 0.86 -0.1 3,692,629 - Psest_3515 0.86 -0.2 3,692,629 - Psest_3515 0.86 -1.2 3,692,629 - Psest_3515 0.86 -2.4 3,692,629 - Psest_3515 0.86 -1.3 3,692,670 + Psest_3515 0.89 +0.3 3,692,687 - -2.0 3,693,150 - Psest_3516 0.18 -2.2 3,693,152 + Psest_3516 0.18 -3.0 3,693,152 + Psest_3516 0.18 -3.4 3,693,153 - Psest_3516 0.18 -4.5 3,693,186 - Psest_3516 0.21 -1.9 3,693,186 - Psest_3516 0.21 -3.6 3,693,356 + Psest_3516 0.37 -4.2 3,693,356 + Psest_3516 0.37 -2.7 3,693,356 + Psest_3516 0.37 -3.1 3,693,357 - Psest_3516 0.37 -2.6 3,693,720 + Psest_3516 0.70 -5.4 3,693,720 + Psest_3516 0.70 -4.4 3,693,721 - Psest_3516 0.70 -1.9 3,693,721 - Psest_3516 0.70 -3.6 3,693,723 - Psest_3516 0.70 -2.2 3,693,723 - Psest_3516 0.70 -1.4 3,693,723 - Psest_3516 0.70 -2.4 3,693,908 + Psest_3516 0.87 -2.8 3,694,099 + -2.1 3,694,100 - -1.1 3,694,100 - +1.1 3,694,100 - +0.7 3,694,100 - -0.2 3,694,362 + -2.8 3,694,611 + Psest_3517 0.12 -3.9 3,694,612 - Psest_3517 0.12 -2.9 3,694,612 - Psest_3517 0.12 -2.9 3,694,662 + Psest_3517 0.13 -2.2 3,694,662 + Psest_3517 0.13 -2.5 3,694,662 + Psest_3517 0.13 -1.5 3,694,680 + Psest_3517 0.14 -4.0 3,694,692 + Psest_3517 0.14 -3.2 3,694,692 + Psest_3517 0.14 -3.8 3,694,693 - Psest_3517 0.14 -5.7 3,694,710 + Psest_3517 0.15 -4.4 3,694,710 + Psest_3517 0.15 -4.7 3,694,710 + Psest_3517 0.15 -3.7 3,694,710 + Psest_3517 0.15 -2.6 3,694,710 + Psest_3517 0.15 -2.8 3,694,710 + Psest_3517 0.15 -3.9 3,694,711 - Psest_3517 0.15 -2.0 3,694,711 - Psest_3517 0.15 -3.2 3,694,711 - Psest_3517 0.15 -0.9 3,694,711 - Psest_3517 0.15 -5.5 3,694,711 - Psest_3517 0.15 -4.0 3,694,711 - Psest_3517 0.15 -2.6 3,694,711 - Psest_3517 0.15 -3.0 3,694,714 - Psest_3517 0.15 -4.8
Or see this region's nucleotide sequence