Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0312 and Psest_0313 are separated by 236 nucleotides Psest_0313 and Psest_0314 are separated by 86 nucleotides
Psest_0312: Psest_0312 - Superfamily II DNA and RNA helicases, at 334,149 to 335,966
_0312
Psest_0313: Psest_0313 - 5,10-methylenetetrahydrofolate reductase, prokaryotic form, at 336,203 to 337,132
_0313
Psest_0314: Psest_0314 - adenosylhomocysteinase, at 337,219 to 338,625
_0314
Position (kb) 336
337
338 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 335.212 kb on - strand, within Psest_0312 at 335.662 kb on + strand, within Psest_0312 at 335.662 kb on + strand, within Psest_0312 at 335.662 kb on + strand, within Psest_0312 at 335.663 kb on - strand, within Psest_0312 at 335.663 kb on - strand, within Psest_0312 at 335.970 kb on + strand at 335.970 kb on + strand at 335.970 kb on + strand at 335.971 kb on - strand at 336.061 kb on + strand at 336.061 kb on + strand at 336.061 kb on + strand at 336.061 kb on + strand at 336.061 kb on + strand at 336.062 kb on - strand at 336.062 kb on - strand at 336.062 kb on - strand at 336.062 kb on - strand at 336.065 kb on + strand at 336.065 kb on + strand at 336.065 kb on + strand at 336.065 kb on + strand at 336.065 kb on + strand at 336.065 kb on + strand at 336.065 kb on + strand at 336.066 kb on - strand at 336.066 kb on - strand at 336.066 kb on - strand at 336.066 kb on - strand at 336.066 kb on - strand at 336.066 kb on - strand at 336.066 kb on - strand at 336.066 kb on - strand at 336.066 kb on - strand at 336.066 kb on - strand at 336.066 kb on - strand at 336.066 kb on - strand at 336.066 kb on - strand at 336.066 kb on - strand at 336.066 kb on - strand at 336.066 kb on - strand at 336.066 kb on - strand at 336.067 kb on + strand at 336.067 kb on + strand at 336.067 kb on + strand at 336.068 kb on - strand at 336.068 kb on - strand at 336.086 kb on + strand at 336.108 kb on + strand at 336.108 kb on + strand at 336.109 kb on - strand at 336.109 kb on - strand at 336.109 kb on - strand at 336.109 kb on - strand at 336.110 kb on + strand at 336.110 kb on + strand at 336.110 kb on + strand at 336.111 kb on - strand at 336.178 kb on + strand at 336.179 kb on - strand at 336.264 kb on + strand at 336.438 kb on + strand, within Psest_0313 at 336.439 kb on - strand, within Psest_0313 at 336.510 kb on + strand, within Psest_0313 at 336.510 kb on + strand, within Psest_0313 at 336.510 kb on + strand, within Psest_0313 at 336.516 kb on + strand, within Psest_0313 at 336.516 kb on + strand, within Psest_0313 at 336.516 kb on + strand, within Psest_0313 at 336.517 kb on - strand, within Psest_0313 at 336.537 kb on + strand, within Psest_0313 at 336.538 kb on - strand, within Psest_0313 at 336.538 kb on - strand, within Psest_0313 at 336.786 kb on + strand, within Psest_0313 at 336.786 kb on + strand, within Psest_0313 at 336.786 kb on + strand, within Psest_0313 at 336.786 kb on + strand, within Psest_0313 at 336.786 kb on + strand, within Psest_0313 at 336.787 kb on - strand, within Psest_0313 at 336.787 kb on - strand, within Psest_0313 at 336.787 kb on - strand, within Psest_0313 at 336.787 kb on - strand, within Psest_0313 at 336.787 kb on - strand, within Psest_0313 at 336.787 kb on - strand, within Psest_0313 at 336.860 kb on + strand, within Psest_0313 at 336.924 kb on + strand, within Psest_0313 at 336.925 kb on - strand, within Psest_0313 at 337.100 kb on + strand at 337.101 kb on - strand at 337.127 kb on + strand at 337.168 kb on - strand at 337.168 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 335,212 - Psest_0312 0.58 -0.6 335,662 + Psest_0312 0.83 +0.6 335,662 + Psest_0312 0.83 +0.9 335,662 + Psest_0312 0.83 +1.1 335,663 - Psest_0312 0.83 -0.4 335,663 - Psest_0312 0.83 +0.7 335,970 + -1.7 335,970 + +0.6 335,970 + -0.9 335,971 - +1.1 336,061 + +0.3 336,061 + -0.6 336,061 + -0.4 336,061 + +0.6 336,061 + +0.1 336,062 - -0.9 336,062 - -0.1 336,062 - +1.0 336,062 - -1.5 336,065 + +1.8 336,065 + +1.9 336,065 + -0.3 336,065 + -0.2 336,065 + -0.3 336,065 + -0.6 336,065 + -0.4 336,066 - -1.4 336,066 - +0.8 336,066 - -0.5 336,066 - +0.7 336,066 - +1.9 336,066 - -0.3 336,066 - -0.4 336,066 - -0.7 336,066 - +0.3 336,066 - +0.9 336,066 - +0.4 336,066 - +1.1 336,066 - -0.2 336,066 - +0.2 336,066 - +1.0 336,066 - +0.1 336,066 - +0.6 336,067 + +0.1 336,067 + +0.4 336,067 + -2.3 336,068 - -0.2 336,068 - -0.8 336,086 + +0.8 336,108 + +0.6 336,108 + +0.9 336,109 - -0.3 336,109 - +0.3 336,109 - -1.9 336,109 - +0.2 336,110 + +0.0 336,110 + -0.8 336,110 + -0.2 336,111 - -0.1 336,178 + -0.6 336,179 - +0.5 336,264 + -1.9 336,438 + Psest_0313 0.25 -2.5 336,439 - Psest_0313 0.25 -1.6 336,510 + Psest_0313 0.33 -1.8 336,510 + Psest_0313 0.33 -4.5 336,510 + Psest_0313 0.33 -2.9 336,516 + Psest_0313 0.34 -2.6 336,516 + Psest_0313 0.34 -1.3 336,516 + Psest_0313 0.34 -2.4 336,517 - Psest_0313 0.34 -2.0 336,537 + Psest_0313 0.36 -2.2 336,538 - Psest_0313 0.36 -3.6 336,538 - Psest_0313 0.36 -0.9 336,786 + Psest_0313 0.63 -3.3 336,786 + Psest_0313 0.63 -2.4 336,786 + Psest_0313 0.63 -2.7 336,786 + Psest_0313 0.63 -2.7 336,786 + Psest_0313 0.63 -3.2 336,787 - Psest_0313 0.63 -2.2 336,787 - Psest_0313 0.63 -2.4 336,787 - Psest_0313 0.63 -1.4 336,787 - Psest_0313 0.63 -3.2 336,787 - Psest_0313 0.63 -0.9 336,787 - Psest_0313 0.63 -3.0 336,860 + Psest_0313 0.71 -2.8 336,924 + Psest_0313 0.78 -3.4 336,925 - Psest_0313 0.78 -1.7 337,100 + -0.8 337,101 - +0.1 337,127 + +0.7 337,168 - +0.1 337,168 - +0.1
Or see this region's nucleotide sequence