Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0291 and Psest_0292 are separated by 57 nucleotides Psest_0292 and Psest_0293 are separated by 22 nucleotides Psest_0293 and Psest_0294 overlap by 4 nucleotides
Psest_0291: Psest_0291 - aspartate carbamoyltransferase, at 306,494 to 307,510
_0291
Psest_0292: Psest_0292 - Pyrimidine operon attenuation protein/uracil phosphoribosyltransferase, at 307,568 to 308,080
_0292
Psest_0293: Psest_0293 - RNAse H-fold protein YqgF, at 308,103 to 308,531
_0293
Psest_0294: Psest_0294 - Putative transcriptional regulator, at 308,528 to 309,097
_0294
Position (kb) 307
308
309 Strain fitness (log2 ratio) -2
-1
0
1 at 306.595 kb on - strand at 306.595 kb on - strand at 306.595 kb on - strand at 306.732 kb on + strand, within Psest_0291 at 306.732 kb on + strand, within Psest_0291 at 306.741 kb on + strand, within Psest_0291 at 307.482 kb on - strand at 307.545 kb on + strand at 307.545 kb on + strand at 307.557 kb on + strand at 307.667 kb on + strand, within Psest_0292 at 307.667 kb on + strand, within Psest_0292 at 307.667 kb on + strand, within Psest_0292 at 307.668 kb on - strand, within Psest_0292 at 307.668 kb on - strand, within Psest_0292 at 307.668 kb on - strand, within Psest_0292 at 308.022 kb on + strand, within Psest_0292 at 308.607 kb on + strand, within Psest_0294 at 309.006 kb on + strand, within Psest_0294 at 309.006 kb on + strand, within Psest_0294 at 309.007 kb on - strand, within Psest_0294 at 309.007 kb on - strand, within Psest_0294 at 309.007 kb on - strand, within Psest_0294 at 309.007 kb on - strand, within Psest_0294 at 309.007 kb on - strand, within Psest_0294 at 309.007 kb on - strand, within Psest_0294 at 309.007 kb on - strand, within Psest_0294 at 309.007 kb on - strand, within Psest_0294
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 306,595 - -0.4 306,595 - -0.9 306,595 - +0.3 306,732 + Psest_0291 0.23 +0.1 306,732 + Psest_0291 0.23 +1.1 306,741 + Psest_0291 0.24 +1.1 307,482 - -0.9 307,545 + -0.1 307,545 + -0.4 307,557 + +0.8 307,667 + Psest_0292 0.19 +0.3 307,667 + Psest_0292 0.19 -1.0 307,667 + Psest_0292 0.19 -1.2 307,668 - Psest_0292 0.19 +0.5 307,668 - Psest_0292 0.19 +0.4 307,668 - Psest_0292 0.19 +0.9 308,022 + Psest_0292 0.88 -1.8 308,607 + Psest_0294 0.14 -0.9 309,006 + Psest_0294 0.84 +0.4 309,006 + Psest_0294 0.84 +0.1 309,007 - Psest_0294 0.84 -0.2 309,007 - Psest_0294 0.84 -0.4 309,007 - Psest_0294 0.84 +0.5 309,007 - Psest_0294 0.84 -0.3 309,007 - Psest_0294 0.84 -1.2 309,007 - Psest_0294 0.84 +0.2 309,007 - Psest_0294 0.84 -0.5 309,007 - Psest_0294 0.84 -0.6
Or see this region's nucleotide sequence