Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2944 and Psest_2945 are separated by 158 nucleotides Psest_2945 and Psest_2946 are separated by 95 nucleotides Psest_2946 and Psest_2947 overlap by 4 nucleotides Psest_2947 and Psest_2948 are separated by 61 nucleotides
Psest_2944: Psest_2944 - lipid A biosynthesis lauroyl (or palmitoleoyl) acyltransferase, at 3,141,414 to 3,142,355
_2944
Psest_2945: Psest_2945 - septum site-determining protein MinC, at 3,142,514 to 3,143,239
_2945
Psest_2946: Psest_2946 - septum site-determining protein MinD, at 3,143,335 to 3,144,150
_2946
Psest_2947: Psest_2947 - cell division topological specificity factor MinE, at 3,144,147 to 3,144,404
_2947
Psest_2948: Psest_2948 - pseudouridine synthase, RluA family, at 3,144,466 to 3,145,101
_2948
Position (kb)
3143
3144
3145 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3142.359 kb on + strand at 3142.481 kb on + strand at 3142.481 kb on + strand at 3142.481 kb on + strand at 3142.481 kb on + strand at 3142.481 kb on + strand at 3142.481 kb on + strand at 3142.481 kb on + strand at 3142.481 kb on + strand at 3142.481 kb on + strand at 3142.482 kb on - strand at 3142.482 kb on - strand at 3142.482 kb on - strand at 3142.482 kb on - strand at 3142.482 kb on - strand at 3142.482 kb on - strand at 3142.482 kb on - strand at 3142.482 kb on - strand at 3142.497 kb on - strand at 3143.039 kb on + strand, within Psest_2945 at 3143.040 kb on - strand, within Psest_2945 at 3143.071 kb on + strand, within Psest_2945 at 3143.071 kb on + strand, within Psest_2945 at 3143.071 kb on + strand, within Psest_2945 at 3143.244 kb on + strand at 3143.379 kb on + strand at 3143.379 kb on + strand at 3143.380 kb on - strand at 3143.506 kb on + strand, within Psest_2946 at 3143.507 kb on - strand, within Psest_2946 at 3143.677 kb on + strand, within Psest_2946 at 3143.677 kb on + strand, within Psest_2946 at 3143.677 kb on + strand, within Psest_2946 at 3143.678 kb on - strand, within Psest_2946 at 3143.731 kb on + strand, within Psest_2946 at 3143.731 kb on + strand, within Psest_2946 at 3143.731 kb on + strand, within Psest_2946 at 3143.731 kb on + strand, within Psest_2946 at 3143.731 kb on + strand, within Psest_2946 at 3143.731 kb on + strand, within Psest_2946 at 3143.731 kb on + strand, within Psest_2946 at 3143.732 kb on - strand, within Psest_2946 at 3143.756 kb on + strand, within Psest_2946 at 3143.867 kb on + strand, within Psest_2946 at 3143.878 kb on + strand, within Psest_2946 at 3143.879 kb on - strand, within Psest_2946 at 3144.047 kb on - strand, within Psest_2946 at 3144.309 kb on + strand, within Psest_2947 at 3144.310 kb on - strand, within Psest_2947 at 3144.420 kb on - strand at 3144.475 kb on - strand at 3144.655 kb on - strand, within Psest_2948 at 3144.655 kb on - strand, within Psest_2948 at 3144.731 kb on - strand, within Psest_2948 at 3144.731 kb on - strand, within Psest_2948 at 3144.731 kb on - strand, within Psest_2948 at 3144.836 kb on + strand, within Psest_2948 at 3144.836 kb on + strand, within Psest_2948 at 3144.836 kb on + strand, within Psest_2948 at 3144.836 kb on + strand, within Psest_2948 at 3144.836 kb on + strand, within Psest_2948 at 3144.836 kb on + strand, within Psest_2948 at 3144.837 kb on - strand, within Psest_2948 at 3144.837 kb on - strand, within Psest_2948 at 3144.837 kb on - strand, within Psest_2948 at 3144.837 kb on - strand, within Psest_2948 at 3144.837 kb on - strand, within Psest_2948 at 3144.837 kb on - strand, within Psest_2948 at 3144.982 kb on + strand, within Psest_2948 at 3144.983 kb on - strand, within Psest_2948 at 3144.983 kb on - strand, within Psest_2948 at 3145.099 kb on + strand at 3145.100 kb on - strand at 3145.100 kb on - strand at 3145.100 kb on - strand at 3145.100 kb on - strand at 3145.100 kb on - strand at 3145.100 kb on - strand at 3145.100 kb on - strand at 3145.100 kb on - strand at 3145.100 kb on - strand at 3145.102 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,142,359 + +1.9 3,142,481 + +0.7 3,142,481 + -0.8 3,142,481 + +0.5 3,142,481 + +0.5 3,142,481 + +0.9 3,142,481 + -0.2 3,142,481 + +0.6 3,142,481 + +0.8 3,142,481 + +0.2 3,142,482 - +0.4 3,142,482 - +0.8 3,142,482 - -0.9 3,142,482 - -1.0 3,142,482 - +1.0 3,142,482 - +0.9 3,142,482 - -0.7 3,142,482 - -1.9 3,142,497 - -1.6 3,143,039 + Psest_2945 0.72 +1.0 3,143,040 - Psest_2945 0.72 -0.1 3,143,071 + Psest_2945 0.77 +1.9 3,143,071 + Psest_2945 0.77 -0.3 3,143,071 + Psest_2945 0.77 +0.3 3,143,244 + -0.1 3,143,379 + +1.5 3,143,379 + -0.7 3,143,380 - -0.3 3,143,506 + Psest_2946 0.21 -1.0 3,143,507 - Psest_2946 0.21 -0.7 3,143,677 + Psest_2946 0.42 -1.3 3,143,677 + Psest_2946 0.42 +2.3 3,143,677 + Psest_2946 0.42 -1.3 3,143,678 - Psest_2946 0.42 -0.7 3,143,731 + Psest_2946 0.49 +2.4 3,143,731 + Psest_2946 0.49 +0.3 3,143,731 + Psest_2946 0.49 -1.3 3,143,731 + Psest_2946 0.49 -0.8 3,143,731 + Psest_2946 0.49 -0.7 3,143,731 + Psest_2946 0.49 +1.9 3,143,731 + Psest_2946 0.49 +1.9 3,143,732 - Psest_2946 0.49 +0.6 3,143,756 + Psest_2946 0.52 -0.1 3,143,867 + Psest_2946 0.65 -0.1 3,143,878 + Psest_2946 0.67 +1.0 3,143,879 - Psest_2946 0.67 +0.9 3,144,047 - Psest_2946 0.87 -0.9 3,144,309 + Psest_2947 0.63 -0.5 3,144,310 - Psest_2947 0.63 -0.7 3,144,420 - -0.0 3,144,475 - +1.0 3,144,655 - Psest_2948 0.30 -0.1 3,144,655 - Psest_2948 0.30 -0.4 3,144,731 - Psest_2948 0.42 -0.3 3,144,731 - Psest_2948 0.42 +0.5 3,144,731 - Psest_2948 0.42 -0.3 3,144,836 + Psest_2948 0.58 +0.3 3,144,836 + Psest_2948 0.58 +0.7 3,144,836 + Psest_2948 0.58 -0.1 3,144,836 + Psest_2948 0.58 +0.7 3,144,836 + Psest_2948 0.58 +1.0 3,144,836 + Psest_2948 0.58 +0.1 3,144,837 - Psest_2948 0.58 -1.0 3,144,837 - Psest_2948 0.58 +0.2 3,144,837 - Psest_2948 0.58 -1.0 3,144,837 - Psest_2948 0.58 -0.2 3,144,837 - Psest_2948 0.58 +0.9 3,144,837 - Psest_2948 0.58 -0.7 3,144,982 + Psest_2948 0.81 +0.4 3,144,983 - Psest_2948 0.81 -0.2 3,144,983 - Psest_2948 0.81 -0.7 3,145,099 + +0.9 3,145,100 - -0.4 3,145,100 - -0.4 3,145,100 - -0.1 3,145,100 - -0.3 3,145,100 - +0.3 3,145,100 - +0.0 3,145,100 - -2.3 3,145,100 - +0.3 3,145,100 - +0.1 3,145,102 + +0.2
Or see this region's nucleotide sequence