Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2435 and Psest_2436 are separated by 156 nucleotides Psest_2436 and Psest_2437 are separated by 47 nucleotides Psest_2437 and Psest_2438 are separated by 36 nucleotides
Psest_2435: Psest_2435 - Transcriptional regulator, at 2,602,886 to 2,603,776
_2435
Psest_2436: Psest_2436 - Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27) (from data) , at 2,603,933 to 2,605,444
_2436
Psest_2437: Psest_2437 - Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) (from data) , at 2,605,492 to 2,606,265
_2437
Psest_2438: Psest_2438 - 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31) (from data) , at 2,606,302 to 2,607,192
_2438
Position (kb)
2603
2604
2605
2606 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2602.936 kb on - strand at 2603.205 kb on + strand, within Psest_2435 at 2603.205 kb on + strand, within Psest_2435 at 2603.206 kb on - strand, within Psest_2435 at 2603.206 kb on - strand, within Psest_2435 at 2603.206 kb on - strand, within Psest_2435 at 2603.268 kb on + strand, within Psest_2435 at 2603.268 kb on + strand, within Psest_2435 at 2603.269 kb on - strand, within Psest_2435 at 2603.283 kb on + strand, within Psest_2435 at 2603.283 kb on + strand, within Psest_2435 at 2603.284 kb on - strand, within Psest_2435 at 2603.284 kb on - strand, within Psest_2435 at 2603.284 kb on - strand, within Psest_2435 at 2603.284 kb on - strand, within Psest_2435 at 2603.614 kb on - strand, within Psest_2435 at 2603.754 kb on + strand at 2603.754 kb on + strand at 2603.755 kb on - strand at 2603.839 kb on + strand at 2603.861 kb on - strand at 2603.933 kb on - strand at 2603.933 kb on - strand at 2603.933 kb on - strand at 2603.956 kb on + strand at 2604.164 kb on + strand, within Psest_2436 at 2604.164 kb on + strand, within Psest_2436 at 2604.165 kb on - strand, within Psest_2436 at 2604.165 kb on - strand, within Psest_2436 at 2604.165 kb on - strand, within Psest_2436 at 2604.165 kb on - strand, within Psest_2436 at 2604.350 kb on + strand, within Psest_2436 at 2604.351 kb on - strand, within Psest_2436 at 2604.351 kb on - strand, within Psest_2436 at 2604.351 kb on - strand, within Psest_2436 at 2604.351 kb on - strand, within Psest_2436 at 2604.351 kb on - strand, within Psest_2436 at 2604.351 kb on - strand, within Psest_2436 at 2604.351 kb on - strand, within Psest_2436 at 2604.651 kb on - strand, within Psest_2436 at 2604.651 kb on - strand, within Psest_2436 at 2605.083 kb on - strand, within Psest_2436 at 2605.260 kb on + strand, within Psest_2436 at 2605.358 kb on + strand at 2605.358 kb on + strand at 2605.359 kb on - strand at 2605.359 kb on - strand at 2605.359 kb on - strand at 2605.359 kb on - strand at 2605.542 kb on + strand at 2605.714 kb on + strand, within Psest_2437 at 2605.726 kb on + strand, within Psest_2437 at 2605.727 kb on - strand, within Psest_2437 at 2605.727 kb on - strand, within Psest_2437 at 2605.898 kb on + strand, within Psest_2437 at 2606.280 kb on + strand at 2606.316 kb on + strand at 2606.317 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,602,936 - -2.0 2,603,205 + Psest_2435 0.36 -0.3 2,603,205 + Psest_2435 0.36 +0.9 2,603,206 - Psest_2435 0.36 -0.1 2,603,206 - Psest_2435 0.36 +1.1 2,603,206 - Psest_2435 0.36 +0.1 2,603,268 + Psest_2435 0.43 -0.2 2,603,268 + Psest_2435 0.43 -0.8 2,603,269 - Psest_2435 0.43 -0.1 2,603,283 + Psest_2435 0.45 -1.1 2,603,283 + Psest_2435 0.45 -0.4 2,603,284 - Psest_2435 0.45 -0.6 2,603,284 - Psest_2435 0.45 +0.8 2,603,284 - Psest_2435 0.45 +0.1 2,603,284 - Psest_2435 0.45 -0.6 2,603,614 - Psest_2435 0.82 -0.9 2,603,754 + +0.2 2,603,754 + +0.5 2,603,755 - -0.6 2,603,839 + +1.0 2,603,861 - -0.1 2,603,933 - -1.2 2,603,933 - -1.4 2,603,933 - -3.8 2,603,956 + -0.5 2,604,164 + Psest_2436 0.15 -1.3 2,604,164 + Psest_2436 0.15 +0.7 2,604,165 - Psest_2436 0.15 -2.7 2,604,165 - Psest_2436 0.15 -3.1 2,604,165 - Psest_2436 0.15 -2.3 2,604,165 - Psest_2436 0.15 -2.7 2,604,350 + Psest_2436 0.28 +0.0 2,604,351 - Psest_2436 0.28 -1.3 2,604,351 - Psest_2436 0.28 -1.5 2,604,351 - Psest_2436 0.28 -2.7 2,604,351 - Psest_2436 0.28 -3.0 2,604,351 - Psest_2436 0.28 -1.6 2,604,351 - Psest_2436 0.28 -2.9 2,604,351 - Psest_2436 0.28 -2.4 2,604,651 - Psest_2436 0.47 -2.9 2,604,651 - Psest_2436 0.47 -1.4 2,605,083 - Psest_2436 0.76 -1.6 2,605,260 + Psest_2436 0.88 -0.0 2,605,358 + -0.2 2,605,358 + -1.0 2,605,359 - -0.8 2,605,359 - -0.5 2,605,359 - -1.3 2,605,359 - -0.9 2,605,542 + -1.0 2,605,714 + Psest_2437 0.29 -1.4 2,605,726 + Psest_2437 0.30 -2.0 2,605,727 - Psest_2437 0.30 -2.1 2,605,727 - Psest_2437 0.30 -2.3 2,605,898 + Psest_2437 0.52 -1.3 2,606,280 + -2.5 2,606,316 + -1.1 2,606,317 - +0.0
Or see this region's nucleotide sequence