Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_1261

Experiment: L-Alanine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_1259 and Psest_1260 are separated by 11 nucleotidesPsest_1260 and Psest_1261 overlap by 4 nucleotidesPsest_1261 and Psest_1262 are separated by 7 nucleotides Psest_1259: Psest_1259 - 23S rRNA m2A2503 methyltransferase, at 1,363,907 to 1,365,055 _1259 Psest_1260: Psest_1260 - type IV pilus biogenesis/stability protein PilW, at 1,365,067 to 1,365,825 _1260 Psest_1261: Psest_1261 - Uncharacterized protein conserved in bacteria, at 1,365,822 to 1,366,832 _1261 Psest_1262: Psest_1262 - 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase, at 1,366,840 to 1,367,952 _1262 Position (kb) 1365 1366 1367Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 1364.990 kb on + strandat 1364.990 kb on + strandat 1364.990 kb on + strandat 1364.990 kb on + strandat 1364.990 kb on + strandat 1365.009 kb on + strandat 1365.009 kb on + strandat 1365.095 kb on + strandat 1365.095 kb on + strandat 1365.095 kb on + strandat 1365.184 kb on + strand, within Psest_1260at 1365.184 kb on + strand, within Psest_1260at 1365.184 kb on + strand, within Psest_1260at 1365.184 kb on + strand, within Psest_1260at 1365.184 kb on + strand, within Psest_1260at 1365.186 kb on + strand, within Psest_1260at 1365.394 kb on + strand, within Psest_1260at 1365.394 kb on + strand, within Psest_1260at 1365.472 kb on + strand, within Psest_1260at 1365.472 kb on + strand, within Psest_1260at 1365.472 kb on + strand, within Psest_1260at 1365.555 kb on + strand, within Psest_1260at 1365.634 kb on + strand, within Psest_1260at 1365.634 kb on + strand, within Psest_1260at 1365.634 kb on + strand, within Psest_1260at 1365.634 kb on + strand, within Psest_1260at 1365.655 kb on + strand, within Psest_1260at 1365.805 kb on + strandat 1365.805 kb on + strandat 1365.998 kb on + strand, within Psest_1261at 1365.998 kb on + strand, within Psest_1261at 1365.998 kb on + strand, within Psest_1261at 1365.998 kb on + strand, within Psest_1261at 1365.998 kb on + strand, within Psest_1261at 1365.998 kb on + strand, within Psest_1261at 1365.998 kb on + strand, within Psest_1261at 1365.998 kb on + strand, within Psest_1261at 1365.998 kb on + strand, within Psest_1261at 1365.998 kb on + strand, within Psest_1261at 1365.998 kb on + strand, within Psest_1261at 1365.998 kb on + strand, within Psest_1261at 1366.183 kb on + strand, within Psest_1261at 1366.726 kb on + strand, within Psest_1261at 1366.726 kb on + strand, within Psest_1261at 1366.726 kb on + strand, within Psest_1261at 1366.726 kb on + strand, within Psest_1261at 1366.726 kb on + strand, within Psest_1261at 1366.726 kb on + strand, within Psest_1261at 1366.726 kb on + strand, within Psest_1261at 1366.761 kb on + strandat 1366.761 kb on + strandat 1366.761 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Alanine (C)
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1,364,990 + -2.0
1,364,990 + -0.0
1,364,990 + -1.2
1,364,990 + -0.4
1,364,990 + +0.1
1,365,009 + -1.3
1,365,009 + -0.9
1,365,095 + -0.3
1,365,095 + +1.6
1,365,095 + +1.2
1,365,184 + Psest_1260 0.15 +1.2
1,365,184 + Psest_1260 0.15 +1.9
1,365,184 + Psest_1260 0.15 +0.4
1,365,184 + Psest_1260 0.15 +0.5
1,365,184 + Psest_1260 0.15 -0.2
1,365,186 + Psest_1260 0.16 +1.2
1,365,394 + Psest_1260 0.43 -0.7
1,365,394 + Psest_1260 0.43 +0.9
1,365,472 + Psest_1260 0.53 +0.0
1,365,472 + Psest_1260 0.53 +0.4
1,365,472 + Psest_1260 0.53 +0.7
1,365,555 + Psest_1260 0.64 +0.1
1,365,634 + Psest_1260 0.75 +1.2
1,365,634 + Psest_1260 0.75 +1.1
1,365,634 + Psest_1260 0.75 -0.0
1,365,634 + Psest_1260 0.75 -0.1
1,365,655 + Psest_1260 0.77 -2.1
1,365,805 + +1.4
1,365,805 + -1.0
1,365,998 + Psest_1261 0.17 +2.9
1,365,998 + Psest_1261 0.17 +3.1
1,365,998 + Psest_1261 0.17 +2.1
1,365,998 + Psest_1261 0.17 +2.1
1,365,998 + Psest_1261 0.17 +3.3
1,365,998 + Psest_1261 0.17 +3.3
1,365,998 + Psest_1261 0.17 +0.7
1,365,998 + Psest_1261 0.17 +3.3
1,365,998 + Psest_1261 0.17 +3.1
1,365,998 + Psest_1261 0.17 +0.9
1,365,998 + Psest_1261 0.17 -1.0
1,365,998 + Psest_1261 0.17 +4.2
1,366,183 + Psest_1261 0.36 +1.3
1,366,726 + Psest_1261 0.89 +0.1
1,366,726 + Psest_1261 0.89 +0.9
1,366,726 + Psest_1261 0.89 -0.6
1,366,726 + Psest_1261 0.89 -1.1
1,366,726 + Psest_1261 0.89 +1.0
1,366,726 + Psest_1261 0.89 +0.2
1,366,726 + Psest_1261 0.89 +0.9
1,366,761 + -0.2
1,366,761 + +0.8
1,366,761 + +1.0

Or see this region's nucleotide sequence