Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0170 and Psest_0172 are separated by 218 nucleotides Psest_0172 and Psest_0173 overlap by 4 nucleotides Psest_0173 and Psest_0174 are separated by 129 nucleotides Psest_0174 and Psest_0175 are separated by 16 nucleotides
Psest_0170: Psest_0170 - 5,10-methenyltetrahydrofolate synthetase, at 166,013 to 166,615
_0170
Psest_0172: Psest_0172 - Uncharacterized protein conserved in bacteria, at 166,834 to 167,148
_0172
Psest_0173: Psest_0173 - TIGR02449 family protein, at 167,145 to 167,354
_0173
Psest_0174: Psest_0174 - Uncharacterized protein conserved in bacteria, at 167,484 to 168,038
_0174
Psest_0175: Psest_0175 - Xaa-Pro aminopeptidase, at 168,055 to 169,389
_0175
Position (kb) 167
168
169 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 166.649 kb on + strand at 166.650 kb on - strand at 166.683 kb on + strand at 166.695 kb on + strand at 166.696 kb on - strand at 166.696 kb on - strand at 166.731 kb on + strand at 166.731 kb on + strand at 166.731 kb on + strand at 166.731 kb on + strand at 166.731 kb on + strand at 166.732 kb on - strand at 166.732 kb on - strand at 166.732 kb on - strand at 166.732 kb on - strand at 166.737 kb on + strand at 166.737 kb on + strand at 166.749 kb on + strand at 166.750 kb on - strand at 166.809 kb on + strand at 166.809 kb on + strand at 166.809 kb on + strand at 166.809 kb on + strand at 166.810 kb on - strand at 166.810 kb on - strand at 166.810 kb on - strand at 166.810 kb on - strand at 166.810 kb on - strand at 166.897 kb on + strand, within Psest_0172 at 166.897 kb on + strand, within Psest_0172 at 166.897 kb on + strand, within Psest_0172 at 166.897 kb on + strand, within Psest_0172 at 166.897 kb on + strand, within Psest_0172 at 166.897 kb on + strand, within Psest_0172 at 166.897 kb on + strand, within Psest_0172 at 166.897 kb on + strand, within Psest_0172 at 166.897 kb on + strand, within Psest_0172 at 166.898 kb on - strand, within Psest_0172 at 166.898 kb on - strand, within Psest_0172 at 166.898 kb on - strand, within Psest_0172 at 166.898 kb on - strand, within Psest_0172 at 166.898 kb on - strand, within Psest_0172 at 166.898 kb on - strand, within Psest_0172 at 166.898 kb on - strand, within Psest_0172 at 166.898 kb on - strand, within Psest_0172 at 166.898 kb on - strand, within Psest_0172 at 166.898 kb on - strand, within Psest_0172 at 166.898 kb on - strand, within Psest_0172 at 167.102 kb on + strand, within Psest_0172 at 167.102 kb on + strand, within Psest_0172 at 167.102 kb on + strand, within Psest_0172 at 167.103 kb on - strand at 167.103 kb on - strand, within Psest_0172 at 167.103 kb on - strand, within Psest_0172 at 167.433 kb on - strand at 167.433 kb on - strand at 167.454 kb on - strand at 167.454 kb on - strand at 167.454 kb on - strand at 167.454 kb on - strand at 167.940 kb on + strand, within Psest_0174 at 167.940 kb on + strand, within Psest_0174 at 167.941 kb on - strand, within Psest_0174 at 167.941 kb on - strand, within Psest_0174 at 167.941 kb on - strand, within Psest_0174 at 167.958 kb on + strand, within Psest_0174 at 167.958 kb on + strand, within Psest_0174 at 167.959 kb on - strand, within Psest_0174 at 167.959 kb on - strand, within Psest_0174 at 167.959 kb on - strand, within Psest_0174 at 167.959 kb on - strand, within Psest_0174 at 168.154 kb on + strand at 168.154 kb on + strand at 168.154 kb on + strand at 168.154 kb on + strand at 168.154 kb on + strand at 168.154 kb on + strand at 168.154 kb on + strand at 168.274 kb on + strand, within Psest_0175 at 168.348 kb on + strand, within Psest_0175 at 168.436 kb on + strand, within Psest_0175 at 168.437 kb on - strand, within Psest_0175 at 168.440 kb on - strand, within Psest_0175 at 168.784 kb on + strand, within Psest_0175 at 168.856 kb on + strand, within Psest_0175 at 168.877 kb on - strand, within Psest_0175 at 168.933 kb on - strand, within Psest_0175 at 168.963 kb on + strand, within Psest_0175
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 166,649 + +0.3 166,650 - -0.7 166,683 + +0.4 166,695 + +0.6 166,696 - +0.4 166,696 - -1.4 166,731 + +0.4 166,731 + +0.4 166,731 + +1.0 166,731 + -0.1 166,731 + +0.8 166,732 - +0.3 166,732 - +0.7 166,732 - -0.5 166,732 - -0.1 166,737 + +1.0 166,737 + +0.4 166,749 + +0.6 166,750 - +0.3 166,809 + +0.9 166,809 + +1.1 166,809 + -0.5 166,809 + +1.7 166,810 - -0.5 166,810 - +1.5 166,810 - -0.1 166,810 - -0.3 166,810 - -0.8 166,897 + Psest_0172 0.20 +1.1 166,897 + Psest_0172 0.20 +0.2 166,897 + Psest_0172 0.20 -1.1 166,897 + Psest_0172 0.20 -0.6 166,897 + Psest_0172 0.20 -1.1 166,897 + Psest_0172 0.20 -1.6 166,897 + Psest_0172 0.20 -0.7 166,897 + Psest_0172 0.20 +0.6 166,897 + Psest_0172 0.20 -1.6 166,898 - Psest_0172 0.20 -1.5 166,898 - Psest_0172 0.20 -1.0 166,898 - Psest_0172 0.20 +1.2 166,898 - Psest_0172 0.20 +1.4 166,898 - Psest_0172 0.20 -0.5 166,898 - Psest_0172 0.20 -0.1 166,898 - Psest_0172 0.20 -0.3 166,898 - Psest_0172 0.20 +0.7 166,898 - Psest_0172 0.20 -1.7 166,898 - Psest_0172 0.20 +1.6 166,898 - Psest_0172 0.20 -0.3 167,102 + Psest_0172 0.85 -0.1 167,102 + Psest_0172 0.85 +0.8 167,102 + Psest_0172 0.85 -0.5 167,103 - +1.1 167,103 - Psest_0172 0.85 -0.5 167,103 - Psest_0172 0.85 +1.0 167,433 - -0.8 167,433 - -0.7 167,454 - -1.5 167,454 - -2.8 167,454 - -0.5 167,454 - -0.7 167,940 + Psest_0174 0.82 -1.4 167,940 + Psest_0174 0.82 -1.5 167,941 - Psest_0174 0.82 -0.6 167,941 - Psest_0174 0.82 -1.5 167,941 - Psest_0174 0.82 -1.5 167,958 + Psest_0174 0.85 +0.1 167,958 + Psest_0174 0.85 +0.7 167,959 - Psest_0174 0.86 -3.0 167,959 - Psest_0174 0.86 -1.2 167,959 - Psest_0174 0.86 -1.5 167,959 - Psest_0174 0.86 -0.6 168,154 + -0.9 168,154 + -0.9 168,154 + -0.1 168,154 + -0.0 168,154 + -0.9 168,154 + -0.4 168,154 + +1.4 168,274 + Psest_0175 0.16 +0.5 168,348 + Psest_0175 0.22 +0.4 168,436 + Psest_0175 0.29 +0.5 168,437 - Psest_0175 0.29 -0.1 168,440 - Psest_0175 0.29 +1.7 168,784 + Psest_0175 0.55 +2.2 168,856 + Psest_0175 0.60 -1.3 168,877 - Psest_0175 0.62 +0.7 168,933 - Psest_0175 0.66 -2.3 168,963 + Psest_0175 0.68 +1.0
Or see this region's nucleotide sequence