Experiment: Ying_OrganicAcid7 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_02750 and IAI47_02755 are separated by 168 nucleotides IAI47_02755 and IAI47_02760 are separated by 130 nucleotides IAI47_02760 and IAI47_02765 are separated by 102 nucleotides
IAI47_02750: IAI47_02750 - class 1 fructose-bisphosphatase, at 570,317 to 571,324
_02750
IAI47_02755: IAI47_02755 - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase, at 571,493 to 572,845
_02755
IAI47_02760: IAI47_02760 - ribosome-associated protein, at 572,976 to 573,521
_02760
IAI47_02765: IAI47_02765 - metalloprotease PmbA, at 573,624 to 574,976
_02765
Position (kb) 571
572
573 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 570.505 kb on - strand, within IAI47_02750 at 570.515 kb on + strand, within IAI47_02750 at 570.515 kb on + strand, within IAI47_02750 at 570.515 kb on + strand, within IAI47_02750 at 570.515 kb on + strand, within IAI47_02750 at 570.515 kb on + strand, within IAI47_02750 at 570.515 kb on + strand, within IAI47_02750 at 570.516 kb on - strand, within IAI47_02750 at 570.516 kb on - strand, within IAI47_02750 at 570.516 kb on - strand, within IAI47_02750 at 570.516 kb on - strand, within IAI47_02750 at 570.516 kb on - strand, within IAI47_02750 at 570.542 kb on - strand, within IAI47_02750 at 570.542 kb on - strand, within IAI47_02750 at 570.555 kb on + strand, within IAI47_02750 at 570.555 kb on + strand, within IAI47_02750 at 570.555 kb on + strand, within IAI47_02750 at 570.555 kb on + strand, within IAI47_02750 at 570.555 kb on + strand, within IAI47_02750 at 570.555 kb on + strand, within IAI47_02750 at 570.555 kb on + strand, within IAI47_02750 at 570.555 kb on + strand, within IAI47_02750 at 570.555 kb on + strand, within IAI47_02750 at 570.555 kb on + strand, within IAI47_02750 at 570.555 kb on + strand, within IAI47_02750 at 570.555 kb on + strand, within IAI47_02750 at 570.555 kb on + strand, within IAI47_02750 at 570.555 kb on + strand, within IAI47_02750 at 570.555 kb on + strand, within IAI47_02750 at 570.555 kb on + strand, within IAI47_02750 at 570.555 kb on + strand, within IAI47_02750 at 570.556 kb on - strand, within IAI47_02750 at 570.556 kb on - strand, within IAI47_02750 at 570.556 kb on - strand, within IAI47_02750 at 570.556 kb on - strand, within IAI47_02750 at 570.556 kb on - strand, within IAI47_02750 at 570.556 kb on - strand, within IAI47_02750 at 570.556 kb on - strand, within IAI47_02750 at 570.556 kb on - strand, within IAI47_02750 at 570.556 kb on - strand, within IAI47_02750 at 570.556 kb on - strand, within IAI47_02750 at 570.556 kb on - strand, within IAI47_02750 at 570.556 kb on - strand, within IAI47_02750 at 570.556 kb on - strand at 570.556 kb on - strand, within IAI47_02750 at 570.556 kb on - strand, within IAI47_02750 at 570.556 kb on - strand, within IAI47_02750 at 570.556 kb on - strand, within IAI47_02750 at 570.556 kb on - strand, within IAI47_02750 at 570.556 kb on - strand, within IAI47_02750 at 570.556 kb on - strand, within IAI47_02750 at 570.560 kb on - strand, within IAI47_02750 at 570.560 kb on - strand, within IAI47_02750 at 570.560 kb on - strand, within IAI47_02750 at 570.560 kb on - strand, within IAI47_02750 at 570.577 kb on + strand, within IAI47_02750 at 570.577 kb on + strand, within IAI47_02750 at 570.578 kb on - strand, within IAI47_02750 at 570.609 kb on + strand, within IAI47_02750 at 570.609 kb on + strand, within IAI47_02750 at 570.609 kb on + strand, within IAI47_02750 at 570.609 kb on + strand, within IAI47_02750 at 570.610 kb on - strand, within IAI47_02750 at 570.610 kb on - strand, within IAI47_02750 at 570.610 kb on - strand, within IAI47_02750 at 570.619 kb on + strand, within IAI47_02750 at 570.619 kb on + strand, within IAI47_02750 at 570.619 kb on + strand, within IAI47_02750 at 570.619 kb on + strand, within IAI47_02750 at 570.620 kb on - strand, within IAI47_02750 at 570.620 kb on - strand, within IAI47_02750 at 570.620 kb on - strand, within IAI47_02750 at 570.620 kb on - strand, within IAI47_02750 at 570.620 kb on - strand, within IAI47_02750 at 570.620 kb on - strand, within IAI47_02750 at 570.621 kb on + strand, within IAI47_02750 at 570.621 kb on + strand, within IAI47_02750 at 570.621 kb on + strand, within IAI47_02750 at 570.621 kb on + strand, within IAI47_02750 at 570.621 kb on + strand, within IAI47_02750 at 570.621 kb on + strand, within IAI47_02750 at 570.622 kb on - strand, within IAI47_02750 at 570.622 kb on - strand, within IAI47_02750 at 570.622 kb on - strand, within IAI47_02750 at 570.622 kb on - strand, within IAI47_02750 at 570.696 kb on + strand, within IAI47_02750 at 570.697 kb on - strand, within IAI47_02750 at 570.697 kb on - strand, within IAI47_02750 at 570.697 kb on - strand, within IAI47_02750 at 570.697 kb on - strand, within IAI47_02750 at 570.697 kb on - strand, within IAI47_02750 at 570.700 kb on + strand, within IAI47_02750 at 570.700 kb on + strand, within IAI47_02750 at 570.700 kb on + strand, within IAI47_02750 at 570.700 kb on + strand, within IAI47_02750 at 570.700 kb on + strand, within IAI47_02750 at 570.700 kb on + strand, within IAI47_02750 at 570.700 kb on + strand, within IAI47_02750 at 570.701 kb on - strand, within IAI47_02750 at 570.715 kb on - strand, within IAI47_02750 at 570.717 kb on + strand, within IAI47_02750 at 570.717 kb on + strand, within IAI47_02750 at 570.718 kb on - strand, within IAI47_02750 at 570.723 kb on + strand, within IAI47_02750 at 570.723 kb on + strand, within IAI47_02750 at 570.723 kb on + strand, within IAI47_02750 at 570.724 kb on - strand, within IAI47_02750 at 570.724 kb on - strand, within IAI47_02750 at 570.724 kb on - strand, within IAI47_02750 at 570.739 kb on - strand, within IAI47_02750 at 570.745 kb on + strand, within IAI47_02750 at 570.745 kb on + strand, within IAI47_02750 at 570.745 kb on + strand, within IAI47_02750 at 570.745 kb on + strand, within IAI47_02750 at 570.745 kb on + strand, within IAI47_02750 at 570.745 kb on + strand, within IAI47_02750 at 570.745 kb on + strand, within IAI47_02750 at 570.746 kb on - strand, within IAI47_02750 at 570.746 kb on - strand, within IAI47_02750 at 570.746 kb on - strand, within IAI47_02750 at 570.746 kb on - strand, within IAI47_02750 at 570.746 kb on - strand, within IAI47_02750 at 570.746 kb on - strand, within IAI47_02750 at 570.746 kb on - strand, within IAI47_02750 at 570.767 kb on + strand, within IAI47_02750 at 570.767 kb on + strand, within IAI47_02750 at 570.767 kb on + strand, within IAI47_02750 at 570.767 kb on + strand, within IAI47_02750 at 570.767 kb on + strand, within IAI47_02750 at 570.768 kb on - strand, within IAI47_02750 at 570.768 kb on - strand, within IAI47_02750 at 570.768 kb on - strand, within IAI47_02750 at 570.768 kb on - strand, within IAI47_02750 at 570.768 kb on - strand, within IAI47_02750 at 570.786 kb on + strand, within IAI47_02750 at 570.786 kb on + strand, within IAI47_02750 at 570.786 kb on + strand, within IAI47_02750 at 570.786 kb on + strand, within IAI47_02750 at 570.786 kb on + strand, within IAI47_02750 at 570.786 kb on + strand, within IAI47_02750 at 570.786 kb on + strand, within IAI47_02750 at 570.786 kb on + strand, within IAI47_02750 at 570.787 kb on - strand, within IAI47_02750 at 570.787 kb on - strand, within IAI47_02750 at 570.787 kb on - strand, within IAI47_02750 at 570.787 kb on - strand, within IAI47_02750 at 570.787 kb on - strand, within IAI47_02750 at 570.787 kb on - strand, within IAI47_02750 at 570.790 kb on + strand, within IAI47_02750 at 570.790 kb on + strand, within IAI47_02750 at 570.790 kb on + strand, within IAI47_02750 at 570.790 kb on + strand, within IAI47_02750 at 570.791 kb on - strand, within IAI47_02750 at 570.791 kb on - strand, within IAI47_02750 at 570.791 kb on - strand, within IAI47_02750 at 570.791 kb on - strand, within IAI47_02750 at 570.817 kb on + strand, within IAI47_02750 at 570.817 kb on + strand, within IAI47_02750 at 570.818 kb on - strand, within IAI47_02750 at 570.819 kb on + strand, within IAI47_02750 at 570.819 kb on + strand, within IAI47_02750 at 570.819 kb on + strand, within IAI47_02750 at 570.819 kb on + strand, within IAI47_02750 at 570.819 kb on + strand, within IAI47_02750 at 570.820 kb on - strand, within IAI47_02750 at 570.820 kb on - strand, within IAI47_02750 at 570.820 kb on - strand, within IAI47_02750 at 570.843 kb on + strand, within IAI47_02750 at 570.844 kb on - strand, within IAI47_02750 at 570.852 kb on + strand, within IAI47_02750 at 570.852 kb on + strand, within IAI47_02750 at 570.853 kb on - strand, within IAI47_02750 at 570.853 kb on - strand, within IAI47_02750 at 570.938 kb on - strand, within IAI47_02750 at 570.977 kb on - strand, within IAI47_02750 at 570.977 kb on - strand, within IAI47_02750 at 570.977 kb on - strand, within IAI47_02750 at 571.001 kb on + strand, within IAI47_02750 at 571.002 kb on - strand, within IAI47_02750 at 571.002 kb on - strand, within IAI47_02750 at 571.011 kb on + strand, within IAI47_02750 at 571.011 kb on + strand, within IAI47_02750 at 571.012 kb on - strand, within IAI47_02750 at 571.012 kb on - strand, within IAI47_02750 at 571.012 kb on - strand, within IAI47_02750 at 571.012 kb on - strand, within IAI47_02750 at 571.012 kb on - strand, within IAI47_02750 at 571.012 kb on - strand, within IAI47_02750 at 571.015 kb on + strand, within IAI47_02750 at 571.039 kb on + strand, within IAI47_02750 at 571.156 kb on + strand, within IAI47_02750 at 571.157 kb on - strand, within IAI47_02750 at 571.157 kb on - strand, within IAI47_02750 at 571.183 kb on + strand, within IAI47_02750 at 571.183 kb on + strand, within IAI47_02750 at 571.183 kb on + strand, within IAI47_02750 at 571.183 kb on + strand, within IAI47_02750 at 571.184 kb on - strand, within IAI47_02750 at 571.184 kb on - strand, within IAI47_02750 at 571.190 kb on + strand, within IAI47_02750 at 571.191 kb on - strand, within IAI47_02750 at 571.191 kb on - strand, within IAI47_02750 at 571.191 kb on - strand, within IAI47_02750 at 571.241 kb on - strand at 571.241 kb on - strand at 571.304 kb on - strand at 571.324 kb on + strand at 571.324 kb on + strand at 571.325 kb on - strand at 571.325 kb on - strand at 571.364 kb on + strand at 571.365 kb on - strand at 571.365 kb on - strand at 571.386 kb on + strand at 571.396 kb on - strand at 571.419 kb on + strand at 571.420 kb on - strand at 571.420 kb on - strand at 571.420 kb on - strand at 571.425 kb on + strand at 571.426 kb on - strand at 571.426 kb on - strand at 571.455 kb on + strand at 571.456 kb on - strand at 571.492 kb on + strand at 571.492 kb on + strand at 571.492 kb on + strand at 571.492 kb on + strand at 571.492 kb on + strand at 571.493 kb on - strand at 571.493 kb on - strand at 571.528 kb on + strand at 571.529 kb on - strand at 571.529 kb on - strand at 571.529 kb on - strand at 571.638 kb on + strand, within IAI47_02755 at 571.650 kb on - strand, within IAI47_02755 at 571.650 kb on - strand, within IAI47_02755 at 571.683 kb on + strand, within IAI47_02755 at 571.683 kb on + strand, within IAI47_02755 at 571.683 kb on + strand, within IAI47_02755 at 571.714 kb on + strand, within IAI47_02755 at 571.715 kb on - strand, within IAI47_02755 at 571.754 kb on + strand, within IAI47_02755 at 571.831 kb on + strand, within IAI47_02755 at 571.831 kb on + strand, within IAI47_02755 at 571.832 kb on - strand, within IAI47_02755 at 571.832 kb on - strand, within IAI47_02755 at 571.898 kb on - strand, within IAI47_02755 at 571.898 kb on - strand, within IAI47_02755 at 571.908 kb on + strand, within IAI47_02755 at 571.908 kb on + strand, within IAI47_02755 at 571.908 kb on + strand, within IAI47_02755 at 571.908 kb on + strand, within IAI47_02755 at 571.909 kb on - strand, within IAI47_02755 at 571.922 kb on + strand, within IAI47_02755 at 571.976 kb on + strand, within IAI47_02755 at 571.976 kb on + strand, within IAI47_02755 at 571.976 kb on + strand, within IAI47_02755 at 571.976 kb on + strand, within IAI47_02755 at 571.976 kb on + strand, within IAI47_02755 at 571.977 kb on - strand, within IAI47_02755 at 571.977 kb on - strand, within IAI47_02755 at 572.018 kb on + strand, within IAI47_02755 at 572.018 kb on + strand, within IAI47_02755 at 572.019 kb on - strand, within IAI47_02755 at 572.019 kb on - strand, within IAI47_02755 at 572.165 kb on - strand, within IAI47_02755 at 572.178 kb on + strand, within IAI47_02755 at 572.178 kb on + strand, within IAI47_02755 at 572.416 kb on + strand, within IAI47_02755 at 572.416 kb on + strand, within IAI47_02755 at 572.416 kb on + strand, within IAI47_02755 at 572.417 kb on - strand, within IAI47_02755 at 572.417 kb on - strand, within IAI47_02755 at 572.417 kb on - strand, within IAI47_02755 at 572.471 kb on + strand, within IAI47_02755 at 572.471 kb on + strand, within IAI47_02755 at 572.471 kb on + strand, within IAI47_02755 at 572.471 kb on + strand, within IAI47_02755 at 572.471 kb on + strand, within IAI47_02755 at 572.472 kb on - strand, within IAI47_02755 at 572.472 kb on - strand, within IAI47_02755 at 572.472 kb on - strand, within IAI47_02755 at 572.531 kb on - strand, within IAI47_02755 at 572.551 kb on + strand, within IAI47_02755 at 572.551 kb on + strand, within IAI47_02755 at 572.551 kb on + strand, within IAI47_02755 at 572.563 kb on - strand, within IAI47_02755 at 572.563 kb on - strand, within IAI47_02755 at 572.665 kb on + strand, within IAI47_02755 at 572.665 kb on + strand, within IAI47_02755 at 572.666 kb on - strand, within IAI47_02755 at 572.666 kb on - strand, within IAI47_02755 at 572.701 kb on + strand, within IAI47_02755 at 572.701 kb on + strand, within IAI47_02755 at 572.701 kb on + strand, within IAI47_02755 at 572.701 kb on + strand, within IAI47_02755 at 572.701 kb on + strand, within IAI47_02755 at 572.701 kb on + strand, within IAI47_02755 at 572.701 kb on + strand, within IAI47_02755 at 572.701 kb on + strand, within IAI47_02755 at 572.702 kb on - strand, within IAI47_02755 at 572.702 kb on - strand, within IAI47_02755 at 572.702 kb on - strand, within IAI47_02755 at 572.734 kb on + strand at 572.735 kb on - strand at 572.813 kb on - strand at 572.846 kb on + strand at 572.846 kb on + strand at 572.847 kb on - strand at 572.871 kb on + strand at 572.872 kb on - strand at 572.872 kb on - strand at 572.892 kb on + strand at 572.978 kb on - strand at 572.978 kb on - strand at 572.978 kb on - strand at 573.005 kb on - strand at 573.082 kb on - strand, within IAI47_02760 at 573.249 kb on + strand, within IAI47_02760 at 573.453 kb on - strand, within IAI47_02760 at 573.554 kb on - strand at 573.596 kb on + strand at 573.617 kb on + strand at 573.617 kb on + strand at 573.617 kb on + strand at 573.617 kb on + strand at 573.617 kb on + strand at 573.617 kb on + strand at 573.617 kb on + strand at 573.617 kb on + strand at 573.618 kb on - strand at 573.618 kb on - strand at 573.618 kb on - strand at 573.618 kb on - strand at 573.618 kb on - strand at 573.618 kb on - strand at 573.618 kb on - strand at 573.618 kb on - strand at 573.623 kb on + strand at 573.643 kb on + strand at 573.643 kb on + strand at 573.643 kb on + strand at 573.643 kb on + strand at 573.643 kb on + strand at 573.643 kb on + strand at 573.643 kb on + strand at 573.643 kb on + strand at 573.643 kb on + strand at 573.644 kb on - strand at 573.644 kb on - strand at 573.644 kb on - strand at 573.644 kb on - strand at 573.644 kb on - strand at 573.647 kb on + strand at 573.647 kb on + strand at 573.647 kb on + strand at 573.647 kb on + strand at 573.647 kb on + strand at 573.648 kb on - strand at 573.648 kb on - strand at 573.648 kb on - strand at 573.648 kb on - strand at 573.715 kb on + strand at 573.715 kb on + strand at 573.715 kb on + strand at 573.715 kb on + strand at 573.716 kb on - strand at 573.716 kb on - strand at 573.716 kb on - strand at 573.716 kb on - strand at 573.742 kb on + strand at 573.746 kb on + strand at 573.747 kb on - strand at 573.747 kb on - strand at 573.777 kb on + strand, within IAI47_02765 at 573.834 kb on + strand, within IAI47_02765 at 573.834 kb on + strand, within IAI47_02765 at 573.834 kb on + strand, within IAI47_02765 at 573.835 kb on - strand, within IAI47_02765 at 573.835 kb on - strand, within IAI47_02765 at 573.835 kb on - strand, within IAI47_02765 at 573.835 kb on - strand, within IAI47_02765
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep C; time point 3 remove 570,505 - IAI47_02750 0.19 -0.7 570,515 + IAI47_02750 0.20 -1.3 570,515 + IAI47_02750 0.20 -2.5 570,515 + IAI47_02750 0.20 -1.3 570,515 + IAI47_02750 0.20 -0.7 570,515 + IAI47_02750 0.20 -2.5 570,515 + IAI47_02750 0.20 -3.5 570,516 - IAI47_02750 0.20 -3.1 570,516 - IAI47_02750 0.20 -3.0 570,516 - IAI47_02750 0.20 -2.9 570,516 - IAI47_02750 0.20 -2.0 570,516 - IAI47_02750 0.20 -3.9 570,542 - IAI47_02750 0.22 -2.0 570,542 - IAI47_02750 0.22 -3.4 570,555 + IAI47_02750 0.24 -3.1 570,555 + IAI47_02750 0.24 -3.8 570,555 + IAI47_02750 0.24 -1.3 570,555 + IAI47_02750 0.24 -1.3 570,555 + IAI47_02750 0.24 -2.0 570,555 + IAI47_02750 0.24 -0.7 570,555 + IAI47_02750 0.24 -2.0 570,555 + IAI47_02750 0.24 -1.3 570,555 + IAI47_02750 0.24 -3.1 570,555 + IAI47_02750 0.24 -3.9 570,555 + IAI47_02750 0.24 -2.5 570,555 + IAI47_02750 0.24 -2.0 570,555 + IAI47_02750 0.24 -2.0 570,555 + IAI47_02750 0.24 -1.3 570,555 + IAI47_02750 0.24 -2.3 570,555 + IAI47_02750 0.24 -2.0 570,555 + IAI47_02750 0.24 +0.3 570,556 - IAI47_02750 0.24 -2.9 570,556 - IAI47_02750 0.24 -1.3 570,556 - IAI47_02750 0.24 -2.0 570,556 - IAI47_02750 0.24 -2.3 570,556 - IAI47_02750 0.24 -2.0 570,556 - IAI47_02750 0.24 -2.7 570,556 - IAI47_02750 0.24 -2.3 570,556 - IAI47_02750 0.24 -2.3 570,556 - IAI47_02750 0.24 -2.0 570,556 - IAI47_02750 0.24 -3.7 570,556 - IAI47_02750 0.24 -2.0 570,556 - IAI47_02750 0.24 -2.3 570,556 - -1.1 570,556 - IAI47_02750 0.24 -1.3 570,556 - IAI47_02750 0.24 -3.1 570,556 - IAI47_02750 0.24 -2.3 570,556 - IAI47_02750 0.24 -2.7 570,556 - IAI47_02750 0.24 -2.5 570,556 - IAI47_02750 0.24 -4.5 570,556 - IAI47_02750 0.24 -3.8 570,560 - IAI47_02750 0.24 -2.3 570,560 - IAI47_02750 0.24 -2.7 570,560 - IAI47_02750 0.24 -2.9 570,560 - IAI47_02750 0.24 -2.0 570,577 + IAI47_02750 0.26 -2.7 570,577 + IAI47_02750 0.26 -1.7 570,578 - IAI47_02750 0.26 -3.0 570,609 + IAI47_02750 0.29 -1.7 570,609 + IAI47_02750 0.29 -2.3 570,609 + IAI47_02750 0.29 -2.3 570,609 + IAI47_02750 0.29 -1.7 570,610 - IAI47_02750 0.29 -1.7 570,610 - IAI47_02750 0.29 -2.0 570,610 - IAI47_02750 0.29 -2.7 570,619 + IAI47_02750 0.30 -2.0 570,619 + IAI47_02750 0.30 -1.3 570,619 + IAI47_02750 0.30 -1.7 570,619 + IAI47_02750 0.30 -3.0 570,620 - IAI47_02750 0.30 -2.5 570,620 - IAI47_02750 0.30 -2.7 570,620 - IAI47_02750 0.30 -0.7 570,620 - IAI47_02750 0.30 -2.0 570,620 - IAI47_02750 0.30 -2.0 570,620 - IAI47_02750 0.30 -2.0 570,621 + IAI47_02750 0.30 -3.1 570,621 + IAI47_02750 0.30 -1.3 570,621 + IAI47_02750 0.30 -3.0 570,621 + IAI47_02750 0.30 -1.7 570,621 + IAI47_02750 0.30 -1.3 570,621 + IAI47_02750 0.30 +0.3 570,622 - IAI47_02750 0.30 -1.3 570,622 - IAI47_02750 0.30 -3.0 570,622 - IAI47_02750 0.30 -0.7 570,622 - IAI47_02750 0.30 -4.2 570,696 + IAI47_02750 0.38 -2.3 570,697 - IAI47_02750 0.38 -2.9 570,697 - IAI47_02750 0.38 -1.7 570,697 - IAI47_02750 0.38 -3.3 570,697 - IAI47_02750 0.38 +0.3 570,697 - IAI47_02750 0.38 -3.5 570,700 + IAI47_02750 0.38 -2.9 570,700 + IAI47_02750 0.38 -2.3 570,700 + IAI47_02750 0.38 -0.7 570,700 + IAI47_02750 0.38 -2.5 570,700 + IAI47_02750 0.38 -2.3 570,700 + IAI47_02750 0.38 -1.7 570,700 + IAI47_02750 0.38 -1.7 570,701 - 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IAI47_02765 0.16 +0.0 573,835 - IAI47_02765 0.16 +0.8 573,835 - IAI47_02765 0.16 +0.3
Or see this region's nucleotide sequence