Experiment: TSB plate, 1:1 coculture with AMD2732
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00159 and MPMX20_00160 are separated by 314 nucleotides MPMX20_00160 and MPMX20_00161 are separated by 47 nucleotides
MPMX20_00159: MPMX20_00159 - Periplasmic alpha-amylase, at 162,513 to 164,543
_00159
MPMX20_00160: MPMX20_00160 - hypothetical protein, at 164,858 to 165,682
_00160
MPMX20_00161: MPMX20_00161 - Xylose operon regulatory protein, at 165,730 to 166,908
_00161
Position (kb) 164
165
166 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7
8 at 163.879 kb on + strand, within MPMX20_00159 at 163.879 kb on + strand, within MPMX20_00159 at 163.879 kb on + strand, within MPMX20_00159 at 163.879 kb on - strand, within MPMX20_00159 at 163.908 kb on - strand, within MPMX20_00159 at 163.910 kb on + strand, within MPMX20_00159 at 163.932 kb on + strand, within MPMX20_00159 at 163.997 kb on + strand, within MPMX20_00159 at 164.011 kb on + strand, within MPMX20_00159 at 164.057 kb on + strand, within MPMX20_00159 at 164.086 kb on + strand, within MPMX20_00159 at 164.086 kb on + strand, within MPMX20_00159 at 164.162 kb on - strand, within MPMX20_00159 at 164.162 kb on - strand, within MPMX20_00159 at 164.194 kb on - strand, within MPMX20_00159 at 164.220 kb on + strand, within MPMX20_00159 at 164.220 kb on + strand, within MPMX20_00159 at 164.228 kb on - strand, within MPMX20_00159 at 164.312 kb on - strand, within MPMX20_00159 at 164.394 kb on - strand at 164.407 kb on + strand at 164.415 kb on - strand at 164.422 kb on + strand at 164.431 kb on - strand at 164.494 kb on + strand at 164.494 kb on + strand at 164.494 kb on + strand at 164.494 kb on + strand at 164.494 kb on + strand at 164.494 kb on + strand at 164.494 kb on + strand at 164.494 kb on + strand at 164.502 kb on - strand at 164.502 kb on - strand at 164.502 kb on - strand at 164.502 kb on - strand at 164.502 kb on - strand at 164.502 kb on - strand at 164.502 kb on - strand at 164.502 kb on - strand at 164.502 kb on - strand at 164.582 kb on - strand at 164.585 kb on + strand at 164.585 kb on + strand at 164.585 kb on + strand at 164.717 kb on + strand at 164.717 kb on + strand at 164.717 kb on + strand at 164.717 kb on + strand at 164.717 kb on + strand at 164.717 kb on + strand at 164.717 kb on + strand at 164.725 kb on - strand at 164.725 kb on - strand at 164.725 kb on - strand at 164.725 kb on - strand at 164.725 kb on - strand at 164.725 kb on - strand at 164.757 kb on - strand at 164.816 kb on + strand at 164.826 kb on + strand at 164.834 kb on + strand at 164.842 kb on - strand at 164.842 kb on - strand at 164.842 kb on - strand at 164.862 kb on - strand at 164.881 kb on + strand at 164.894 kb on + strand at 164.924 kb on + strand at 164.951 kb on + strand, within MPMX20_00160 at 164.991 kb on + strand, within MPMX20_00160 at 164.991 kb on + strand, within MPMX20_00160 at 164.991 kb on + strand, within MPMX20_00160 at 164.991 kb on + strand, within MPMX20_00160 at 164.991 kb on + strand, within MPMX20_00160 at 164.991 kb on - strand, within MPMX20_00160 at 164.998 kb on + strand, within MPMX20_00160 at 164.998 kb on + strand, within MPMX20_00160 at 164.999 kb on - strand, within MPMX20_00160 at 164.999 kb on - strand, within MPMX20_00160 at 164.999 kb on - strand, within MPMX20_00160 at 164.999 kb on - strand, within MPMX20_00160 at 165.006 kb on + strand, within MPMX20_00160 at 165.006 kb on - strand, within MPMX20_00160 at 165.037 kb on + strand, within MPMX20_00160 at 165.045 kb on - strand, within MPMX20_00160 at 165.045 kb on - strand, within MPMX20_00160 at 165.045 kb on - strand, within MPMX20_00160 at 165.047 kb on + strand, within MPMX20_00160 at 165.105 kb on + strand, within MPMX20_00160 at 165.113 kb on - strand, within MPMX20_00160 at 165.113 kb on - strand, within MPMX20_00160 at 165.113 kb on - strand, within MPMX20_00160 at 165.209 kb on + strand, within MPMX20_00160 at 165.232 kb on + strand, within MPMX20_00160 at 165.281 kb on + strand, within MPMX20_00160 at 165.281 kb on + strand, within MPMX20_00160 at 165.281 kb on + strand, within MPMX20_00160 at 165.281 kb on + strand, within MPMX20_00160 at 165.281 kb on + strand, within MPMX20_00160 at 165.305 kb on - strand, within MPMX20_00160 at 165.310 kb on - strand, within MPMX20_00160 at 165.312 kb on + strand, within MPMX20_00160 at 165.352 kb on - strand, within MPMX20_00160 at 165.439 kb on + strand, within MPMX20_00160 at 165.442 kb on - strand, within MPMX20_00160 at 165.442 kb on - strand, within MPMX20_00160 at 165.449 kb on - strand, within MPMX20_00160 at 165.511 kb on - strand, within MPMX20_00160 at 165.554 kb on + strand, within MPMX20_00160 at 165.589 kb on + strand, within MPMX20_00160 at 165.610 kb on + strand at 165.611 kb on + strand at 165.611 kb on + strand at 165.643 kb on + strand at 165.701 kb on + strand at 165.753 kb on - strand at 165.765 kb on + strand at 165.778 kb on + strand at 165.778 kb on + strand at 165.786 kb on + strand at 165.794 kb on - strand at 165.794 kb on - strand at 165.794 kb on - strand at 165.794 kb on - strand at 165.827 kb on + strand at 165.827 kb on + strand at 165.830 kb on - strand at 165.835 kb on - strand at 165.835 kb on - strand at 165.863 kb on + strand, within MPMX20_00161 at 165.952 kb on + strand, within MPMX20_00161 at 165.982 kb on + strand, within MPMX20_00161 at 165.988 kb on - strand, within MPMX20_00161 at 165.998 kb on + strand, within MPMX20_00161 at 166.006 kb on - strand, within MPMX20_00161 at 166.087 kb on + strand, within MPMX20_00161 at 166.095 kb on - strand, within MPMX20_00161 at 166.095 kb on - strand, within MPMX20_00161 at 166.106 kb on + strand, within MPMX20_00161 at 166.114 kb on - strand, within MPMX20_00161 at 166.190 kb on + strand, within MPMX20_00161 at 166.190 kb on + strand, within MPMX20_00161 at 166.190 kb on + strand, within MPMX20_00161 at 166.198 kb on - strand, within MPMX20_00161 at 166.198 kb on - strand, within MPMX20_00161 at 166.198 kb on - strand, within MPMX20_00161 at 166.198 kb on - strand, within MPMX20_00161 at 166.237 kb on + strand, within MPMX20_00161 at 166.242 kb on + strand, within MPMX20_00161 at 166.291 kb on + strand, within MPMX20_00161 at 166.299 kb on - strand, within MPMX20_00161 at 166.299 kb on - strand, within MPMX20_00161 at 166.299 kb on - strand, within MPMX20_00161 at 166.299 kb on - strand, within MPMX20_00161 at 166.299 kb on - strand, within MPMX20_00161 at 166.381 kb on + strand, within MPMX20_00161 at 166.424 kb on + strand, within MPMX20_00161 at 166.492 kb on - strand, within MPMX20_00161 at 166.497 kb on + strand, within MPMX20_00161 at 166.510 kb on - strand, within MPMX20_00161 at 166.548 kb on + strand, within MPMX20_00161 at 166.583 kb on + strand, within MPMX20_00161 at 166.583 kb on + strand, within MPMX20_00161 at 166.583 kb on + strand, within MPMX20_00161 at 166.583 kb on + strand, within MPMX20_00161 at 166.591 kb on - strand, within MPMX20_00161 at 166.591 kb on - strand, within MPMX20_00161 at 166.591 kb on - strand, within MPMX20_00161 at 166.591 kb on - strand, within MPMX20_00161 at 166.612 kb on + strand, within MPMX20_00161 at 166.618 kb on - strand, within MPMX20_00161 at 166.633 kb on + strand, within MPMX20_00161
Per-strain Table
Position Strand Gene LocusTag Fraction TSB plate, 1:1 coculture with AMD2732 remove 163,879 + MPMX20_00159 0.67 -1.6 163,879 + MPMX20_00159 0.67 +0.4 163,879 + MPMX20_00159 0.67 -3.4 163,879 - MPMX20_00159 0.67 -0.3 163,908 - MPMX20_00159 0.69 +1.3 163,910 + MPMX20_00159 0.69 -0.0 163,932 + MPMX20_00159 0.70 -1.2 163,997 + MPMX20_00159 0.73 +0.2 164,011 + MPMX20_00159 0.74 +0.3 164,057 + MPMX20_00159 0.76 -0.6 164,086 + MPMX20_00159 0.77 +0.0 164,086 + MPMX20_00159 0.77 +0.4 164,162 - MPMX20_00159 0.81 +0.9 164,162 - MPMX20_00159 0.81 +0.7 164,194 - MPMX20_00159 0.83 -0.5 164,220 + MPMX20_00159 0.84 -1.8 164,220 + MPMX20_00159 0.84 -1.2 164,228 - MPMX20_00159 0.84 -0.3 164,312 - MPMX20_00159 0.89 +0.7 164,394 - -1.8 164,407 + +0.3 164,415 - -0.3 164,422 + -3.5 164,431 - +1.8 164,494 + -3.4 164,494 + -0.3 164,494 + -1.5 164,494 + +0.5 164,494 + -0.1 164,494 + +0.5 164,494 + -2.4 164,494 + -1.4 164,502 - -0.9 164,502 - -1.3 164,502 - -0.3 164,502 - -1.2 164,502 - -0.3 164,502 - -0.3 164,502 - -1.1 164,502 - +0.4 164,502 - +1.2 164,582 - -1.5 164,585 + +1.5 164,585 + -0.4 164,585 + +2.1 164,717 + +0.1 164,717 + -0.2 164,717 + -0.0 164,717 + +0.3 164,717 + +0.9 164,717 + +2.3 164,717 + -0.1 164,725 - -1.8 164,725 - +0.7 164,725 - +1.2 164,725 - -2.0 164,725 - -0.9 164,725 - -1.5 164,757 - -1.6 164,816 + -0.4 164,826 + -0.5 164,834 + +0.2 164,842 - +2.6 164,842 - -1.4 164,842 - +0.7 164,862 - +0.5 164,881 + +0.3 164,894 + -0.5 164,924 + -0.9 164,951 + MPMX20_00160 0.11 -0.5 164,991 + MPMX20_00160 0.16 -0.9 164,991 + MPMX20_00160 0.16 -0.5 164,991 + MPMX20_00160 0.16 -1.5 164,991 + MPMX20_00160 0.16 -0.0 164,991 + MPMX20_00160 0.16 +0.7 164,991 - MPMX20_00160 0.16 +1.0 164,998 + MPMX20_00160 0.17 +1.3 164,998 + MPMX20_00160 0.17 +0.3 164,999 - MPMX20_00160 0.17 -1.4 164,999 - MPMX20_00160 0.17 +1.3 164,999 - MPMX20_00160 0.17 -1.1 164,999 - MPMX20_00160 0.17 -1.2 165,006 + MPMX20_00160 0.18 +0.4 165,006 - MPMX20_00160 0.18 -1.3 165,037 + MPMX20_00160 0.22 -1.8 165,045 - MPMX20_00160 0.23 -0.2 165,045 - MPMX20_00160 0.23 -0.9 165,045 - MPMX20_00160 0.23 +0.2 165,047 + MPMX20_00160 0.23 -0.6 165,105 + MPMX20_00160 0.30 +0.2 165,113 - MPMX20_00160 0.31 +0.3 165,113 - MPMX20_00160 0.31 +0.8 165,113 - MPMX20_00160 0.31 +1.7 165,209 + MPMX20_00160 0.43 -0.9 165,232 + MPMX20_00160 0.45 +1.2 165,281 + MPMX20_00160 0.51 +0.4 165,281 + MPMX20_00160 0.51 -1.1 165,281 + MPMX20_00160 0.51 -0.0 165,281 + MPMX20_00160 0.51 +0.3 165,281 + MPMX20_00160 0.51 -0.4 165,305 - MPMX20_00160 0.54 +0.2 165,310 - MPMX20_00160 0.55 -1.2 165,312 + MPMX20_00160 0.55 +2.3 165,352 - MPMX20_00160 0.60 -1.1 165,439 + MPMX20_00160 0.70 -0.5 165,442 - MPMX20_00160 0.71 -0.6 165,442 - MPMX20_00160 0.71 +1.9 165,449 - MPMX20_00160 0.72 -1.0 165,511 - MPMX20_00160 0.79 -0.6 165,554 + MPMX20_00160 0.84 +8.4 165,589 + MPMX20_00160 0.89 +0.8 165,610 + -1.0 165,611 + +1.3 165,611 + +1.3 165,643 + +0.6 165,701 + -1.0 165,753 - -1.4 165,765 + +0.5 165,778 + -1.3 165,778 + +0.5 165,786 + -1.5 165,794 - -0.1 165,794 - -0.3 165,794 - +1.3 165,794 - +0.3 165,827 + -0.1 165,827 + -0.7 165,830 - -1.8 165,835 - -0.9 165,835 - +0.3 165,863 + MPMX20_00161 0.11 +0.1 165,952 + MPMX20_00161 0.19 -3.1 165,982 + MPMX20_00161 0.21 -0.5 165,988 - MPMX20_00161 0.22 -0.7 165,998 + MPMX20_00161 0.23 -0.3 166,006 - MPMX20_00161 0.23 -1.7 166,087 + MPMX20_00161 0.30 -0.0 166,095 - MPMX20_00161 0.31 +2.3 166,095 - MPMX20_00161 0.31 -1.4 166,106 + MPMX20_00161 0.32 -1.2 166,114 - MPMX20_00161 0.33 +0.7 166,190 + MPMX20_00161 0.39 -0.5 166,190 + MPMX20_00161 0.39 -0.4 166,190 + MPMX20_00161 0.39 -1.4 166,198 - MPMX20_00161 0.40 -1.6 166,198 - MPMX20_00161 0.40 -0.3 166,198 - MPMX20_00161 0.40 -0.9 166,198 - MPMX20_00161 0.40 -1.2 166,237 + MPMX20_00161 0.43 -0.9 166,242 + MPMX20_00161 0.43 -0.4 166,291 + MPMX20_00161 0.48 -1.7 166,299 - MPMX20_00161 0.48 +0.2 166,299 - MPMX20_00161 0.48 -1.1 166,299 - MPMX20_00161 0.48 -0.3 166,299 - MPMX20_00161 0.48 -0.2 166,299 - MPMX20_00161 0.48 +0.8 166,381 + MPMX20_00161 0.55 -1.4 166,424 + MPMX20_00161 0.59 +4.7 166,492 - MPMX20_00161 0.65 +1.1 166,497 + MPMX20_00161 0.65 -0.4 166,510 - MPMX20_00161 0.66 -0.6 166,548 + MPMX20_00161 0.69 +0.2 166,583 + MPMX20_00161 0.72 +1.0 166,583 + MPMX20_00161 0.72 -1.3 166,583 + MPMX20_00161 0.72 -0.3 166,583 + MPMX20_00161 0.72 +1.9 166,591 - MPMX20_00161 0.73 -1.5 166,591 - MPMX20_00161 0.73 -0.0 166,591 - MPMX20_00161 0.73 -2.6 166,591 - MPMX20_00161 0.73 -0.5 166,612 + MPMX20_00161 0.75 +0.7 166,618 - MPMX20_00161 0.75 -1.8 166,633 + MPMX20_00161 0.77 -0.4
Or see this region's nucleotide sequence