Experiment: 0.1X LB plate, 1:1 coculture with Pseudomonas silesiensis TBS_010
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_04314 and MPMX20_04315 are separated by 230 nucleotides MPMX20_04315 and MPMX20_04316 are separated by 177 nucleotides MPMX20_04316 and MPMX20_04317 are separated by 50 nucleotides
MPMX20_04314: MPMX20_04314 - 1,4-alpha-glucan branching enzyme GlgB, at 4,507,159 to 4,509,345
_04314
MPMX20_04315: MPMX20_04315 - Aspartate-semialdehyde dehydrogenase, at 4,509,576 to 4,510,679
_04315
MPMX20_04316: MPMX20_04316 - hypothetical protein, at 4,510,857 to 4,511,450
_04316
MPMX20_04317: MPMX20_04317 - Low-affinity gluconate transporter, at 4,511,501 to 4,512,841
_04317
Position (kb)
4509
4510
4511 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4508.657 kb on - strand, within MPMX20_04314 at 4508.718 kb on - strand, within MPMX20_04314 at 4508.807 kb on + strand, within MPMX20_04314 at 4508.815 kb on - strand, within MPMX20_04314 at 4508.815 kb on - strand, within MPMX20_04314 at 4508.817 kb on + strand, within MPMX20_04314 at 4508.992 kb on - strand, within MPMX20_04314 at 4509.007 kb on - strand, within MPMX20_04314 at 4509.007 kb on - strand, within MPMX20_04314 at 4509.007 kb on - strand, within MPMX20_04314 at 4509.046 kb on + strand, within MPMX20_04314 at 4509.119 kb on - strand, within MPMX20_04314 at 4509.210 kb on + strand at 4509.218 kb on - strand at 4509.223 kb on + strand at 4509.268 kb on + strand at 4509.270 kb on - strand at 4509.324 kb on - strand at 4509.335 kb on + strand at 4509.335 kb on + strand at 4509.392 kb on - strand at 4509.393 kb on + strand at 4509.393 kb on + strand at 4509.393 kb on + strand at 4509.393 kb on + strand at 4509.393 kb on + strand at 4509.401 kb on - strand at 4509.401 kb on - strand at 4509.401 kb on - strand at 4509.401 kb on - strand at 4509.401 kb on - strand at 4509.401 kb on - strand at 4509.420 kb on + strand at 4509.426 kb on - strand at 4509.426 kb on - strand at 4509.428 kb on - strand at 4509.430 kb on + strand at 4509.430 kb on + strand at 4509.430 kb on + strand at 4509.430 kb on + strand at 4509.430 kb on + strand at 4509.433 kb on - strand at 4509.482 kb on + strand at 4509.486 kb on - strand at 4509.555 kb on + strand at 4510.705 kb on + strand at 4510.747 kb on - strand at 4510.838 kb on - strand at 4510.838 kb on - strand at 4510.873 kb on - strand at 4510.913 kb on - strand at 4510.940 kb on + strand, within MPMX20_04316 at 4510.953 kb on + strand, within MPMX20_04316 at 4510.961 kb on - strand, within MPMX20_04316 at 4510.988 kb on + strand, within MPMX20_04316 at 4511.055 kb on - strand, within MPMX20_04316 at 4511.062 kb on + strand, within MPMX20_04316 at 4511.086 kb on + strand, within MPMX20_04316 at 4511.232 kb on - strand, within MPMX20_04316 at 4511.323 kb on - strand, within MPMX20_04316 at 4511.329 kb on - strand, within MPMX20_04316 at 4511.406 kb on + strand at 4511.421 kb on + strand at 4511.438 kb on + strand at 4511.438 kb on + strand at 4511.504 kb on + strand at 4511.525 kb on + strand at 4511.623 kb on + strand at 4511.656 kb on + strand, within MPMX20_04317 at 4511.664 kb on - strand, within MPMX20_04317
Per-strain Table
Position Strand Gene LocusTag Fraction 0.1X LB plate, 1:1 coculture with Pseudomonas silesiensis TBS_010 remove 4,508,657 - MPMX20_04314 0.68 -0.9 4,508,718 - MPMX20_04314 0.71 -1.3 4,508,807 + MPMX20_04314 0.75 -1.4 4,508,815 - MPMX20_04314 0.76 -4.0 4,508,815 - MPMX20_04314 0.76 -2.8 4,508,817 + MPMX20_04314 0.76 -0.0 4,508,992 - MPMX20_04314 0.84 -1.8 4,509,007 - MPMX20_04314 0.84 -0.5 4,509,007 - MPMX20_04314 0.84 -1.1 4,509,007 - MPMX20_04314 0.84 -0.9 4,509,046 + MPMX20_04314 0.86 -1.6 4,509,119 - MPMX20_04314 0.90 -1.0 4,509,210 + -0.8 4,509,218 - -0.6 4,509,223 + -2.0 4,509,268 + -0.4 4,509,270 - +0.2 4,509,324 - -0.8 4,509,335 + +1.1 4,509,335 + -0.9 4,509,392 - -1.4 4,509,393 + -0.5 4,509,393 + -0.2 4,509,393 + +0.0 4,509,393 + +1.0 4,509,393 + -0.4 4,509,401 - -0.0 4,509,401 - -0.2 4,509,401 - -0.2 4,509,401 - -0.1 4,509,401 - +0.7 4,509,401 - -0.0 4,509,420 + -0.1 4,509,426 - -1.4 4,509,426 - +0.2 4,509,428 - +0.0 4,509,430 + -1.3 4,509,430 + +0.2 4,509,430 + -0.2 4,509,430 + -0.6 4,509,430 + +1.0 4,509,433 - -1.3 4,509,482 + +0.0 4,509,486 - -0.2 4,509,555 + -0.9 4,510,705 + +1.0 4,510,747 - -1.7 4,510,838 - -0.4 4,510,838 - +0.4 4,510,873 - -0.8 4,510,913 - +0.8 4,510,940 + MPMX20_04316 0.14 +0.9 4,510,953 + MPMX20_04316 0.16 +1.0 4,510,961 - MPMX20_04316 0.18 -0.4 4,510,988 + MPMX20_04316 0.22 -1.5 4,511,055 - MPMX20_04316 0.33 +1.4 4,511,062 + MPMX20_04316 0.35 -0.3 4,511,086 + MPMX20_04316 0.39 -0.8 4,511,232 - MPMX20_04316 0.63 +0.8 4,511,323 - MPMX20_04316 0.78 +0.3 4,511,329 - MPMX20_04316 0.79 -1.3 4,511,406 + +1.5 4,511,421 + +2.5 4,511,438 + +0.0 4,511,438 + -0.0 4,511,504 + +0.4 4,511,525 + -0.0 4,511,623 + +2.5 4,511,656 + MPMX20_04317 0.12 +0.5 4,511,664 - MPMX20_04317 0.12 -0.8
Or see this region's nucleotide sequence