Experiment: 0.1X LB plate, 1:1 coculture with Pseudomonas silesiensis TBS_010
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_03408 and MPMX20_03409 are separated by 76 nucleotides MPMX20_03409 and MPMX20_03410 are separated by 13 nucleotides MPMX20_03410 and MPMX20_03411 are separated by 23 nucleotides MPMX20_03411 and MPMX20_03412 are separated by 119 nucleotides MPMX20_03412 and MPMX20_03413 are separated by 165 nucleotides
MPMX20_03408: MPMX20_03408 - Co-chaperone protein HscB, at 3,594,838 to 3,595,353
_03408
MPMX20_03409: MPMX20_03409 - Iron-binding protein IscA, at 3,595,430 to 3,595,753
_03409
MPMX20_03410: MPMX20_03410 - Iron-sulfur cluster assembly scaffold protein IscU, at 3,595,767 to 3,596,153
_03410
MPMX20_03411: MPMX20_03411 - Cysteine desulfurase IscS, at 3,596,177 to 3,597,391
_03411
MPMX20_03412: MPMX20_03412 - HTH-type transcriptional regulator IscR, at 3,597,511 to 3,598,002
_03412
MPMX20_03413: MPMX20_03413 - tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ, at 3,598,168 to 3,599,010
_03413
Position (kb)
3596
3597
3598 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3595.186 kb on - strand, within MPMX20_03408 at 3595.219 kb on + strand, within MPMX20_03408 at 3595.328 kb on + strand at 3595.336 kb on - strand at 3595.450 kb on - strand at 3595.464 kb on + strand, within MPMX20_03409 at 3595.467 kb on - strand, within MPMX20_03409 at 3595.528 kb on - strand, within MPMX20_03409 at 3595.557 kb on - strand, within MPMX20_03409 at 3595.585 kb on - strand, within MPMX20_03409 at 3595.633 kb on - strand, within MPMX20_03409 at 3595.687 kb on - strand, within MPMX20_03409 at 3595.749 kb on - strand at 3595.749 kb on - strand at 3595.983 kb on - strand, within MPMX20_03410 at 3596.199 kb on - strand at 3596.199 kb on - strand at 3596.199 kb on - strand at 3596.199 kb on - strand at 3596.201 kb on + strand at 3596.201 kb on + strand at 3596.201 kb on + strand at 3596.201 kb on + strand at 3596.201 kb on + strand at 3596.226 kb on - strand at 3596.245 kb on + strand at 3597.766 kb on - strand, within MPMX20_03412 at 3597.766 kb on - strand, within MPMX20_03412 at 3597.785 kb on - strand, within MPMX20_03412 at 3597.785 kb on - strand, within MPMX20_03412 at 3597.866 kb on - strand, within MPMX20_03412 at 3597.881 kb on - strand, within MPMX20_03412 at 3598.069 kb on - strand at 3598.141 kb on - strand at 3598.267 kb on + strand, within MPMX20_03413 at 3598.315 kb on - strand, within MPMX20_03413 at 3598.334 kb on + strand, within MPMX20_03413 at 3598.334 kb on + strand, within MPMX20_03413 at 3598.342 kb on - strand, within MPMX20_03413 at 3598.364 kb on - strand, within MPMX20_03413 at 3598.381 kb on - strand, within MPMX20_03413
Per-strain Table
Position Strand Gene LocusTag Fraction 0.1X LB plate, 1:1 coculture with Pseudomonas silesiensis TBS_010 remove 3,595,186 - MPMX20_03408 0.67 -1.6 3,595,219 + MPMX20_03408 0.74 -0.3 3,595,328 + +2.0 3,595,336 - -0.5 3,595,450 - -1.4 3,595,464 + MPMX20_03409 0.10 +2.4 3,595,467 - MPMX20_03409 0.11 -0.1 3,595,528 - MPMX20_03409 0.30 -0.2 3,595,557 - MPMX20_03409 0.39 +0.0 3,595,585 - MPMX20_03409 0.48 +2.0 3,595,633 - MPMX20_03409 0.63 -1.4 3,595,687 - MPMX20_03409 0.79 +2.4 3,595,749 - +2.0 3,595,749 - +2.0 3,595,983 - MPMX20_03410 0.56 -1.0 3,596,199 - -1.7 3,596,199 - +1.0 3,596,199 - -0.3 3,596,199 - -0.6 3,596,201 + +0.0 3,596,201 + +0.4 3,596,201 + -1.6 3,596,201 + +0.4 3,596,201 + -1.9 3,596,226 - -2.6 3,596,245 + -0.3 3,597,766 - MPMX20_03412 0.52 -1.0 3,597,766 - MPMX20_03412 0.52 -0.1 3,597,785 - MPMX20_03412 0.56 -0.7 3,597,785 - MPMX20_03412 0.56 +1.1 3,597,866 - MPMX20_03412 0.72 -0.3 3,597,881 - MPMX20_03412 0.75 -0.1 3,598,069 - -0.7 3,598,141 - -0.7 3,598,267 + MPMX20_03413 0.12 +1.0 3,598,315 - MPMX20_03413 0.17 -0.3 3,598,334 + MPMX20_03413 0.20 +0.7 3,598,334 + MPMX20_03413 0.20 -0.9 3,598,342 - MPMX20_03413 0.21 -0.1 3,598,364 - MPMX20_03413 0.23 -0.8 3,598,381 - MPMX20_03413 0.25 -1.8
Or see this region's nucleotide sequence