Experiment: M9 plate, 1:1 coculture with Paraburkholderia phenoluptrix CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_01804 and MPMX20_01805 overlap by 4 nucleotides MPMX20_01805 and MPMX20_01806 overlap by 4 nucleotides MPMX20_01806 and MPMX20_01807 overlap by 17 nucleotides
MPMX20_01804: MPMX20_01804 - Spermidine/putrescine-binding periplasmic protein, at 1,898,810 to 1,899,844
_01804
MPMX20_01805: MPMX20_01805 - Inner membrane ABC transporter permease protein YdcV, at 1,899,841 to 1,900,632
_01805
MPMX20_01806: MPMX20_01806 - Spermidine/putrescine transport system permease protein PotB, at 1,900,629 to 1,901,486
_01806
MPMX20_01807: MPMX20_01807 - Spermidine/putrescine import ATP-binding protein PotA, at 1,901,470 to 1,902,555
_01807
Position (kb)
1899
1900
1901 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1898.894 kb on - strand at 1898.913 kb on - strand at 1898.965 kb on + strand, within MPMX20_01804 at 1898.966 kb on + strand, within MPMX20_01804 at 1899.027 kb on + strand, within MPMX20_01804 at 1899.027 kb on + strand, within MPMX20_01804 at 1899.030 kb on - strand, within MPMX20_01804 at 1899.167 kb on + strand, within MPMX20_01804 at 1899.209 kb on - strand, within MPMX20_01804 at 1899.233 kb on - strand, within MPMX20_01804 at 1899.235 kb on - strand, within MPMX20_01804 at 1899.249 kb on - strand, within MPMX20_01804 at 1899.274 kb on - strand, within MPMX20_01804 at 1899.333 kb on + strand, within MPMX20_01804 at 1899.335 kb on + strand, within MPMX20_01804 at 1899.335 kb on + strand, within MPMX20_01804 at 1899.341 kb on - strand, within MPMX20_01804 at 1899.341 kb on - strand, within MPMX20_01804 at 1899.343 kb on - strand, within MPMX20_01804 at 1899.368 kb on - strand, within MPMX20_01804 at 1899.397 kb on + strand, within MPMX20_01804 at 1899.397 kb on + strand, within MPMX20_01804 at 1899.407 kb on + strand, within MPMX20_01804 at 1899.440 kb on + strand, within MPMX20_01804 at 1899.440 kb on + strand, within MPMX20_01804 at 1899.448 kb on - strand, within MPMX20_01804 at 1899.470 kb on - strand, within MPMX20_01804 at 1899.470 kb on - strand, within MPMX20_01804 at 1899.470 kb on - strand, within MPMX20_01804 at 1899.475 kb on - strand, within MPMX20_01804 at 1899.489 kb on + strand, within MPMX20_01804 at 1899.533 kb on + strand, within MPMX20_01804 at 1899.645 kb on + strand, within MPMX20_01804 at 1899.722 kb on - strand, within MPMX20_01804 at 1899.733 kb on + strand, within MPMX20_01804 at 1899.733 kb on + strand, within MPMX20_01804 at 1899.733 kb on + strand, within MPMX20_01804 at 1899.741 kb on - strand at 1899.920 kb on + strand at 1899.924 kb on + strand, within MPMX20_01805 at 1899.926 kb on + strand, within MPMX20_01805 at 1900.011 kb on - strand, within MPMX20_01805 at 1900.015 kb on + strand, within MPMX20_01805 at 1900.017 kb on + strand, within MPMX20_01805 at 1900.035 kb on - strand, within MPMX20_01805 at 1900.037 kb on - strand, within MPMX20_01805 at 1900.037 kb on - strand, within MPMX20_01805 at 1900.058 kb on - strand, within MPMX20_01805 at 1900.058 kb on - strand, within MPMX20_01805 at 1900.058 kb on - strand, within MPMX20_01805 at 1900.167 kb on + strand, within MPMX20_01805 at 1900.175 kb on - strand, within MPMX20_01805 at 1900.175 kb on - strand, within MPMX20_01805 at 1900.203 kb on + strand, within MPMX20_01805 at 1900.447 kb on + strand, within MPMX20_01805 at 1900.447 kb on + strand, within MPMX20_01805 at 1900.455 kb on - strand, within MPMX20_01805 at 1900.455 kb on - strand, within MPMX20_01805 at 1900.455 kb on - strand, within MPMX20_01805 at 1900.457 kb on + strand, within MPMX20_01805 at 1900.457 kb on + strand, within MPMX20_01805 at 1900.460 kb on - strand, within MPMX20_01805 at 1900.465 kb on - strand, within MPMX20_01805 at 1900.465 kb on - strand, within MPMX20_01805 at 1900.492 kb on + strand, within MPMX20_01805 at 1900.693 kb on - strand at 1900.697 kb on + strand at 1900.717 kb on - strand, within MPMX20_01806 at 1900.798 kb on + strand, within MPMX20_01806 at 1900.816 kb on - strand, within MPMX20_01806 at 1900.841 kb on + strand, within MPMX20_01806 at 1900.898 kb on + strand, within MPMX20_01806 at 1900.999 kb on - strand, within MPMX20_01806 at 1901.006 kb on - strand, within MPMX20_01806 at 1901.008 kb on + strand, within MPMX20_01806 at 1901.144 kb on + strand, within MPMX20_01806 at 1901.246 kb on - strand, within MPMX20_01806 at 1901.252 kb on - strand, within MPMX20_01806 at 1901.270 kb on + strand, within MPMX20_01806 at 1901.305 kb on - strand, within MPMX20_01806 at 1901.354 kb on - strand, within MPMX20_01806 at 1901.434 kb on - strand at 1901.488 kb on + strand at 1901.493 kb on + strand at 1901.501 kb on - strand at 1901.535 kb on + strand at 1901.548 kb on + strand at 1901.629 kb on + strand, within MPMX20_01807 at 1901.631 kb on - strand, within MPMX20_01807
Per-strain Table
Position Strand Gene LocusTag Fraction M9 plate, 1:1 coculture with Paraburkholderia phenoluptrix CO2 remove 1,898,894 - -0.6 1,898,913 - -0.9 1,898,965 + MPMX20_01804 0.15 +3.2 1,898,966 + MPMX20_01804 0.15 -2.0 1,899,027 + MPMX20_01804 0.21 +0.3 1,899,027 + MPMX20_01804 0.21 +1.8 1,899,030 - MPMX20_01804 0.21 +1.2 1,899,167 + MPMX20_01804 0.34 -1.0 1,899,209 - MPMX20_01804 0.39 -0.3 1,899,233 - MPMX20_01804 0.41 -1.3 1,899,235 - MPMX20_01804 0.41 +0.0 1,899,249 - MPMX20_01804 0.42 -3.7 1,899,274 - MPMX20_01804 0.45 +3.0 1,899,333 + MPMX20_01804 0.51 -1.9 1,899,335 + MPMX20_01804 0.51 +0.4 1,899,335 + MPMX20_01804 0.51 -1.7 1,899,341 - MPMX20_01804 0.51 +0.9 1,899,341 - MPMX20_01804 0.51 -0.7 1,899,343 - MPMX20_01804 0.51 -0.6 1,899,368 - MPMX20_01804 0.54 -1.2 1,899,397 + MPMX20_01804 0.57 -1.4 1,899,397 + MPMX20_01804 0.57 -0.4 1,899,407 + MPMX20_01804 0.58 -0.4 1,899,440 + MPMX20_01804 0.61 -0.2 1,899,440 + MPMX20_01804 0.61 -1.3 1,899,448 - MPMX20_01804 0.62 -2.9 1,899,470 - MPMX20_01804 0.64 +0.4 1,899,470 - MPMX20_01804 0.64 -0.9 1,899,470 - MPMX20_01804 0.64 +0.4 1,899,475 - MPMX20_01804 0.64 -1.9 1,899,489 + MPMX20_01804 0.66 -1.0 1,899,533 + MPMX20_01804 0.70 -0.9 1,899,645 + MPMX20_01804 0.81 -1.5 1,899,722 - MPMX20_01804 0.88 -0.2 1,899,733 + MPMX20_01804 0.89 -0.3 1,899,733 + MPMX20_01804 0.89 +1.2 1,899,733 + MPMX20_01804 0.89 -0.9 1,899,741 - -1.9 1,899,920 + -0.2 1,899,924 + MPMX20_01805 0.10 -1.4 1,899,926 + MPMX20_01805 0.11 -1.1 1,900,011 - MPMX20_01805 0.21 -1.1 1,900,015 + MPMX20_01805 0.22 -0.0 1,900,017 + MPMX20_01805 0.22 -0.7 1,900,035 - MPMX20_01805 0.24 -1.1 1,900,037 - MPMX20_01805 0.25 -0.3 1,900,037 - MPMX20_01805 0.25 +0.5 1,900,058 - MPMX20_01805 0.27 -2.4 1,900,058 - MPMX20_01805 0.27 -1.7 1,900,058 - MPMX20_01805 0.27 +3.2 1,900,167 + MPMX20_01805 0.41 +1.7 1,900,175 - MPMX20_01805 0.42 +0.7 1,900,175 - MPMX20_01805 0.42 -1.1 1,900,203 + MPMX20_01805 0.46 -1.8 1,900,447 + MPMX20_01805 0.77 -0.9 1,900,447 + MPMX20_01805 0.77 -2.4 1,900,455 - MPMX20_01805 0.78 -0.2 1,900,455 - MPMX20_01805 0.78 -0.1 1,900,455 - MPMX20_01805 0.78 -0.9 1,900,457 + MPMX20_01805 0.78 +3.2 1,900,457 + MPMX20_01805 0.78 -1.8 1,900,460 - MPMX20_01805 0.78 -0.3 1,900,465 - MPMX20_01805 0.79 -1.4 1,900,465 - MPMX20_01805 0.79 -1.4 1,900,492 + MPMX20_01805 0.82 -0.6 1,900,693 - -1.5 1,900,697 + -0.3 1,900,717 - MPMX20_01806 0.10 -2.3 1,900,798 + MPMX20_01806 0.20 -0.5 1,900,816 - MPMX20_01806 0.22 +0.8 1,900,841 + MPMX20_01806 0.25 -1.7 1,900,898 + MPMX20_01806 0.31 -2.5 1,900,999 - MPMX20_01806 0.43 -1.2 1,901,006 - MPMX20_01806 0.44 -1.4 1,901,008 + MPMX20_01806 0.44 +1.1 1,901,144 + MPMX20_01806 0.60 -1.4 1,901,246 - MPMX20_01806 0.72 +0.1 1,901,252 - MPMX20_01806 0.73 -1.9 1,901,270 + MPMX20_01806 0.75 -0.1 1,901,305 - MPMX20_01806 0.79 +0.7 1,901,354 - MPMX20_01806 0.84 -2.9 1,901,434 - +1.5 1,901,488 + +0.7 1,901,493 + +1.3 1,901,501 - +3.2 1,901,535 + -0.5 1,901,548 + +1.7 1,901,629 + MPMX20_01807 0.15 -1.3 1,901,631 - MPMX20_01807 0.15 +2.5
Or see this region's nucleotide sequence