Experiment: M9 plate, 1:1 coculture with Paraburkholderia phenoluptrix CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_01541 and MPMX20_01542 are separated by 70 nucleotides MPMX20_01542 and MPMX20_01543 are separated by 267 nucleotides MPMX20_01543 and MPMX20_01544 are separated by 28 nucleotides
MPMX20_01541: MPMX20_01541 - Macrolide export ATP-binding/permease protein MacB, at 1,612,499 to 1,614,439
_01541
MPMX20_01542: MPMX20_01542 - Cold shock-like protein CspD, at 1,614,510 to 1,614,731
_01542
MPMX20_01543: MPMX20_01543 - ATP-dependent Clp protease adapter protein ClpS, at 1,614,999 to 1,615,319
_01543
MPMX20_01544: MPMX20_01544 - ATP-dependent Clp protease ATP-binding subunit ClpA, at 1,615,348 to 1,617,627
_01544
Position (kb)
1614
1615
1616 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1614.042 kb on - strand, within MPMX20_01541 at 1614.053 kb on + strand, within MPMX20_01541 at 1614.059 kb on - strand, within MPMX20_01541 at 1614.059 kb on - strand, within MPMX20_01541 at 1614.061 kb on - strand, within MPMX20_01541 at 1614.110 kb on - strand, within MPMX20_01541 at 1614.221 kb on + strand, within MPMX20_01541 at 1614.229 kb on - strand, within MPMX20_01541 at 1614.239 kb on - strand, within MPMX20_01541 at 1614.252 kb on + strand at 1614.252 kb on + strand at 1614.435 kb on - strand at 1614.495 kb on + strand at 1614.503 kb on - strand at 1614.503 kb on - strand at 1614.503 kb on - strand at 1614.545 kb on + strand, within MPMX20_01542 at 1614.609 kb on - strand, within MPMX20_01542 at 1614.642 kb on - strand, within MPMX20_01542 at 1614.681 kb on - strand, within MPMX20_01542 at 1614.699 kb on - strand, within MPMX20_01542 at 1614.718 kb on - strand at 1614.718 kb on - strand at 1614.743 kb on - strand at 1614.743 kb on - strand at 1614.786 kb on + strand at 1614.794 kb on - strand at 1614.796 kb on + strand at 1614.796 kb on + strand at 1614.796 kb on + strand at 1614.796 kb on + strand at 1614.804 kb on - strand at 1614.804 kb on - strand at 1614.804 kb on - strand at 1614.804 kb on - strand at 1614.901 kb on + strand at 1614.978 kb on + strand at 1615.015 kb on - strand at 1615.079 kb on + strand, within MPMX20_01543 at 1615.102 kb on - strand, within MPMX20_01543 at 1615.252 kb on - strand, within MPMX20_01543 at 1615.392 kb on - strand at 1615.414 kb on + strand at 1615.417 kb on - strand at 1615.424 kb on - strand at 1615.424 kb on - strand at 1615.455 kb on - strand at 1615.519 kb on + strand at 1615.562 kb on + strand at 1615.562 kb on + strand at 1615.629 kb on + strand, within MPMX20_01544 at 1615.645 kb on - strand, within MPMX20_01544 at 1615.672 kb on - strand, within MPMX20_01544 at 1615.763 kb on + strand, within MPMX20_01544 at 1615.839 kb on - strand, within MPMX20_01544 at 1615.909 kb on - strand, within MPMX20_01544 at 1615.930 kb on + strand, within MPMX20_01544 at 1616.052 kb on - strand, within MPMX20_01544 at 1616.168 kb on + strand, within MPMX20_01544 at 1616.176 kb on - strand, within MPMX20_01544 at 1616.235 kb on + strand, within MPMX20_01544 at 1616.310 kb on + strand, within MPMX20_01544
Per-strain Table
Position Strand Gene LocusTag Fraction M9 plate, 1:1 coculture with Paraburkholderia phenoluptrix CO2 remove 1,614,042 - MPMX20_01541 0.79 -0.2 1,614,053 + MPMX20_01541 0.80 +1.2 1,614,059 - MPMX20_01541 0.80 -0.1 1,614,059 - MPMX20_01541 0.80 -0.2 1,614,061 - MPMX20_01541 0.80 -1.5 1,614,110 - MPMX20_01541 0.83 +0.6 1,614,221 + MPMX20_01541 0.89 -0.4 1,614,229 - MPMX20_01541 0.89 -0.5 1,614,239 - MPMX20_01541 0.90 -1.1 1,614,252 + -0.6 1,614,252 + -0.5 1,614,435 - -0.9 1,614,495 + -2.7 1,614,503 - +0.2 1,614,503 - -1.1 1,614,503 - +3.2 1,614,545 + MPMX20_01542 0.16 -0.2 1,614,609 - MPMX20_01542 0.45 -0.4 1,614,642 - MPMX20_01542 0.59 -0.1 1,614,681 - MPMX20_01542 0.77 +0.6 1,614,699 - MPMX20_01542 0.85 -1.2 1,614,718 - -2.5 1,614,718 - +0.2 1,614,743 - +0.0 1,614,743 - +0.2 1,614,786 + -0.9 1,614,794 - -1.1 1,614,796 + -1.5 1,614,796 + +0.8 1,614,796 + -0.9 1,614,796 + -2.1 1,614,804 - +1.6 1,614,804 - +0.4 1,614,804 - +3.2 1,614,804 - -1.2 1,614,901 + -0.1 1,614,978 + -1.4 1,615,015 - +1.5 1,615,079 + MPMX20_01543 0.25 -2.9 1,615,102 - MPMX20_01543 0.32 +0.0 1,615,252 - MPMX20_01543 0.79 -0.8 1,615,392 - -1.2 1,615,414 + +0.9 1,615,417 - -1.3 1,615,424 - -1.0 1,615,424 - +0.4 1,615,455 - +0.4 1,615,519 + -0.7 1,615,562 + +2.2 1,615,562 + +0.9 1,615,629 + MPMX20_01544 0.12 -0.8 1,615,645 - MPMX20_01544 0.13 -0.1 1,615,672 - MPMX20_01544 0.14 -0.7 1,615,763 + MPMX20_01544 0.18 +0.3 1,615,839 - MPMX20_01544 0.22 -2.1 1,615,909 - MPMX20_01544 0.25 +0.1 1,615,930 + MPMX20_01544 0.26 +0.5 1,616,052 - MPMX20_01544 0.31 -0.4 1,616,168 + MPMX20_01544 0.36 -1.3 1,616,176 - MPMX20_01544 0.36 +0.8 1,616,235 + MPMX20_01544 0.39 -1.3 1,616,310 + MPMX20_01544 0.42 -0.1
Or see this region's nucleotide sequence