Experiment: M9 plate, 1:1 coculture with Paraburkholderia phenoluptrix CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_01294 and MPMX20_01295 are separated by 28 nucleotides MPMX20_01295 and MPMX20_01296 are separated by 3 nucleotides MPMX20_01296 and MPMX20_01297 are separated by 270 nucleotides MPMX20_01297 and MPMX20_01298 are separated by 1 nucleotides
MPMX20_01294: MPMX20_01294 - Peptidoglycan D,D-transpeptidase MrdA, at 1,354,930 to 1,356,831
_01294
MPMX20_01295: MPMX20_01295 - Ribosomal RNA large subunit methyltransferase H, at 1,356,860 to 1,357,327
_01295
MPMX20_01296: MPMX20_01296 - Ribosomal silencing factor RsfS, at 1,357,331 to 1,357,648
_01296
MPMX20_01297: MPMX20_01297 - Nicotinate-nucleotide adenylyltransferase, at 1,357,919 to 1,358,575
_01297
MPMX20_01298: MPMX20_01298 - DNA polymerase III subunit delta, at 1,358,577 to 1,359,608
_01298
Position (kb)
1357
1358 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6 at 1356.874 kb on - strand at 1356.881 kb on - strand at 1357.009 kb on - strand, within MPMX20_01295 at 1357.028 kb on - strand, within MPMX20_01295 at 1357.117 kb on - strand, within MPMX20_01295 at 1357.151 kb on - strand, within MPMX20_01295 at 1357.342 kb on - strand at 1357.483 kb on - strand, within MPMX20_01296 at 1357.564 kb on - strand, within MPMX20_01296 at 1357.593 kb on - strand, within MPMX20_01296 at 1357.593 kb on - strand, within MPMX20_01296 at 1357.594 kb on - strand, within MPMX20_01296 at 1357.594 kb on - strand, within MPMX20_01296 at 1357.643 kb on - strand at 1357.643 kb on - strand at 1357.643 kb on - strand at 1357.669 kb on - strand at 1357.700 kb on + strand at 1357.710 kb on - strand at 1357.713 kb on - strand at 1357.713 kb on - strand at 1357.767 kb on + strand at 1357.775 kb on - strand at 1357.851 kb on + strand at 1357.853 kb on + strand at 1357.853 kb on + strand at 1357.859 kb on - strand at 1357.861 kb on - strand at 1357.861 kb on - strand at 1357.861 kb on - strand at 1357.865 kb on + strand at 1357.865 kb on + strand at 1357.865 kb on + strand at 1357.865 kb on + strand at 1357.865 kb on + strand at 1357.865 kb on + strand at 1357.867 kb on - strand at 1357.867 kb on - strand at 1357.873 kb on - strand at 1357.873 kb on - strand at 1357.873 kb on - strand at 1357.873 kb on - strand at 1357.873 kb on - strand at 1357.962 kb on - strand at 1358.614 kb on - strand at 1358.624 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 plate, 1:1 coculture with Paraburkholderia phenoluptrix CO2 remove 1,356,874 - -1.0 1,356,881 - +6.0 1,357,009 - MPMX20_01295 0.32 -1.1 1,357,028 - MPMX20_01295 0.36 +0.9 1,357,117 - MPMX20_01295 0.55 +0.7 1,357,151 - MPMX20_01295 0.62 +0.4 1,357,342 - +1.0 1,357,483 - MPMX20_01296 0.48 -0.2 1,357,564 - MPMX20_01296 0.73 -2.0 1,357,593 - MPMX20_01296 0.82 -1.1 1,357,593 - MPMX20_01296 0.82 -1.0 1,357,594 - MPMX20_01296 0.83 -1.3 1,357,594 - MPMX20_01296 0.83 -2.1 1,357,643 - +0.0 1,357,643 - -0.7 1,357,643 - +0.7 1,357,669 - +0.2 1,357,700 + +2.2 1,357,710 - -1.5 1,357,713 - -1.5 1,357,713 - -2.0 1,357,767 + -1.8 1,357,775 - -1.0 1,357,851 + +1.9 1,357,853 + +1.3 1,357,853 + -0.9 1,357,859 - -1.4 1,357,861 - +1.0 1,357,861 - -1.1 1,357,861 - +0.4 1,357,865 + +0.4 1,357,865 + -0.0 1,357,865 + -1.0 1,357,865 + +0.9 1,357,865 + -2.0 1,357,865 + +1.2 1,357,867 - -0.5 1,357,867 - -2.2 1,357,873 - +0.6 1,357,873 - -1.5 1,357,873 - -0.0 1,357,873 - +0.5 1,357,873 - -2.5 1,357,962 - +0.9 1,358,614 - -2.1 1,358,624 - +0.0
Or see this region's nucleotide sequence