Experiment: M9 plate, 1:1 coculture with Paraburkholderia phenoluptrix CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00842 and MPMX20_00843 are separated by 56 nucleotides MPMX20_00843 and MPMX20_00844 are separated by 27 nucleotides MPMX20_00844 and MPMX20_00845 overlap by 8 nucleotides MPMX20_00845 and MPMX20_00846 are separated by 82 nucleotides
MPMX20_00842: MPMX20_00842 - Iron-sulfur cluster insertion protein ErpA, at 889,383 to 889,727
_00842
MPMX20_00843: MPMX20_00843 - hypothetical protein, at 889,784 to 890,407
_00843
MPMX20_00844: MPMX20_00844 - Vitamin B12-binding protein, at 890,435 to 891,235
_00844
MPMX20_00845: MPMX20_00845 - 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase, at 891,228 to 891,926
_00845
MPMX20_00846: MPMX20_00846 - Deoxyguanosinetriphosphate triphosphohydrolase, at 892,009 to 893,523
_00846
Position (kb) 890
891
892 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7
8
9
10 at 889.754 kb on + strand at 889.762 kb on - strand at 889.856 kb on - strand, within MPMX20_00843 at 889.860 kb on - strand, within MPMX20_00843 at 889.907 kb on + strand, within MPMX20_00843 at 889.915 kb on - strand, within MPMX20_00843 at 890.004 kb on - strand, within MPMX20_00843 at 890.046 kb on - strand, within MPMX20_00843 at 890.196 kb on - strand, within MPMX20_00843 at 890.297 kb on - strand, within MPMX20_00843 at 890.442 kb on + strand at 890.457 kb on + strand at 890.457 kb on + strand at 890.457 kb on + strand at 890.457 kb on + strand at 890.457 kb on + strand at 890.465 kb on - strand at 890.467 kb on + strand at 890.509 kb on + strand at 890.538 kb on - strand, within MPMX20_00844 at 890.629 kb on + strand, within MPMX20_00844 at 890.634 kb on + strand, within MPMX20_00844 at 890.637 kb on - strand, within MPMX20_00844 at 890.710 kb on + strand, within MPMX20_00844 at 890.729 kb on + strand, within MPMX20_00844 at 890.732 kb on + strand, within MPMX20_00844 at 890.851 kb on + strand, within MPMX20_00844 at 890.859 kb on - strand, within MPMX20_00844 at 890.859 kb on - strand, within MPMX20_00844 at 890.864 kb on - strand, within MPMX20_00844 at 890.864 kb on - strand, within MPMX20_00844 at 890.896 kb on + strand, within MPMX20_00844 at 890.896 kb on + strand, within MPMX20_00844 at 890.904 kb on - strand, within MPMX20_00844 at 890.904 kb on - strand, within MPMX20_00844 at 891.050 kb on - strand, within MPMX20_00844 at 891.060 kb on - strand, within MPMX20_00844 at 891.083 kb on - strand, within MPMX20_00844 at 891.097 kb on + strand, within MPMX20_00844 at 891.105 kb on + strand, within MPMX20_00844 at 891.218 kb on + strand at 891.238 kb on - strand at 891.994 kb on - strand at 892.003 kb on + strand at 892.093 kb on - strand at 892.124 kb on + strand at 892.131 kb on - strand at 892.225 kb on + strand, within MPMX20_00846
Per-strain Table
Position Strand Gene LocusTag Fraction M9 plate, 1:1 coculture with Paraburkholderia phenoluptrix CO2 remove 889,754 + -1.8 889,762 - +0.8 889,856 - MPMX20_00843 0.12 +0.1 889,860 - MPMX20_00843 0.12 -0.7 889,907 + MPMX20_00843 0.20 -2.0 889,915 - MPMX20_00843 0.21 -1.4 890,004 - MPMX20_00843 0.35 +0.2 890,046 - MPMX20_00843 0.42 -0.0 890,196 - MPMX20_00843 0.66 -0.7 890,297 - MPMX20_00843 0.82 +0.8 890,442 + -0.1 890,457 + +1.7 890,457 + -0.7 890,457 + +2.7 890,457 + -0.6 890,457 + +0.8 890,465 - -2.3 890,467 + +0.8 890,509 + +1.8 890,538 - MPMX20_00844 0.13 +0.0 890,629 + MPMX20_00844 0.24 +0.2 890,634 + MPMX20_00844 0.25 -0.1 890,637 - MPMX20_00844 0.25 +0.1 890,710 + MPMX20_00844 0.34 -1.5 890,729 + MPMX20_00844 0.37 +1.5 890,732 + MPMX20_00844 0.37 -0.4 890,851 + MPMX20_00844 0.52 -0.8 890,859 - MPMX20_00844 0.53 +0.3 890,859 - MPMX20_00844 0.53 -1.0 890,864 - MPMX20_00844 0.54 -1.5 890,864 - MPMX20_00844 0.54 -1.7 890,896 + MPMX20_00844 0.58 -1.7 890,896 + MPMX20_00844 0.58 +0.0 890,904 - MPMX20_00844 0.59 -1.8 890,904 - MPMX20_00844 0.59 -1.1 891,050 - MPMX20_00844 0.77 -0.5 891,060 - MPMX20_00844 0.78 +10.7 891,083 - MPMX20_00844 0.81 -0.3 891,097 + MPMX20_00844 0.83 +1.1 891,105 + MPMX20_00844 0.84 -0.8 891,218 + -1.1 891,238 - -2.9 891,994 - -0.5 892,003 + -1.6 892,093 - +0.3 892,124 + -1.4 892,131 - -1.0 892,225 + MPMX20_00846 0.14 -0.4
Or see this region's nucleotide sequence