Experiment: M9 plate, 1:1 coculture with Paraburkholderia phenoluptrix CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00385 and MPMX20_00386 are separated by 20 nucleotides MPMX20_00386 and MPMX20_00387 are separated by 11 nucleotides MPMX20_00387 and MPMX20_00388 are separated by 53 nucleotides MPMX20_00388 and MPMX20_00389 are separated by 87 nucleotides MPMX20_00389 and MPMX20_00390 are separated by 40 nucleotides
MPMX20_00385: MPMX20_00385 - hypothetical protein, at 405,677 to 406,540
_00385
MPMX20_00386: MPMX20_00386 - hypothetical protein, at 406,561 to 406,692
_00386
MPMX20_00387: MPMX20_00387 - hypothetical protein, at 406,704 to 406,814
_00387
MPMX20_00388: MPMX20_00388 - hypothetical protein, at 406,868 to 406,966
_00388
MPMX20_00389: MPMX20_00389 - hypothetical protein, at 407,054 to 407,440
_00389
MPMX20_00390: MPMX20_00390 - hypothetical protein, at 407,481 to 407,897
_00390
Position (kb) 406
407 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15 at 405.712 kb on - strand at 405.718 kb on + strand at 405.735 kb on + strand at 405.736 kb on - strand at 405.736 kb on - strand at 405.743 kb on - strand at 405.769 kb on + strand, within MPMX20_00385 at 405.861 kb on + strand, within MPMX20_00385 at 405.899 kb on - strand, within MPMX20_00385 at 405.918 kb on + strand, within MPMX20_00385 at 405.923 kb on + strand, within MPMX20_00385 at 405.940 kb on - strand, within MPMX20_00385 at 405.987 kb on + strand, within MPMX20_00385 at 406.019 kb on + strand, within MPMX20_00385 at 406.055 kb on + strand, within MPMX20_00385 at 406.125 kb on + strand, within MPMX20_00385 at 406.155 kb on + strand, within MPMX20_00385 at 406.155 kb on + strand, within MPMX20_00385 at 406.181 kb on - strand, within MPMX20_00385 at 406.203 kb on - strand, within MPMX20_00385 at 406.222 kb on - strand, within MPMX20_00385 at 406.224 kb on + strand, within MPMX20_00385 at 406.236 kb on + strand, within MPMX20_00385 at 406.327 kb on + strand, within MPMX20_00385 at 406.365 kb on + strand, within MPMX20_00385 at 406.373 kb on - strand, within MPMX20_00385 at 406.375 kb on + strand, within MPMX20_00385 at 406.375 kb on + strand, within MPMX20_00385 at 406.378 kb on - strand, within MPMX20_00385 at 406.487 kb on + strand at 406.487 kb on + strand at 406.487 kb on + strand at 406.495 kb on - strand at 406.519 kb on + strand at 406.534 kb on + strand at 406.534 kb on + strand at 406.534 kb on + strand at 406.556 kb on + strand at 406.564 kb on - strand at 406.573 kb on - strand at 406.601 kb on - strand, within MPMX20_00386 at 406.668 kb on + strand, within MPMX20_00386 at 406.668 kb on + strand, within MPMX20_00386 at 406.676 kb on - strand, within MPMX20_00386 at 406.676 kb on - strand, within MPMX20_00386 at 406.715 kb on + strand, within MPMX20_00387 at 406.772 kb on - strand, within MPMX20_00387 at 406.772 kb on - strand, within MPMX20_00387 at 406.772 kb on - strand, within MPMX20_00387 at 406.774 kb on + strand, within MPMX20_00387 at 406.774 kb on + strand, within MPMX20_00387 at 406.777 kb on - strand, within MPMX20_00387 at 406.782 kb on - strand, within MPMX20_00387 at 406.827 kb on + strand at 406.872 kb on + strand at 406.893 kb on + strand, within MPMX20_00388 at 406.966 kb on + strand at 406.976 kb on + strand at 406.981 kb on + strand at 406.981 kb on + strand at 406.981 kb on + strand at 406.981 kb on + strand at 406.981 kb on + strand at 406.984 kb on - strand at 406.989 kb on - strand at 407.052 kb on - strand at 407.067 kb on + strand at 407.074 kb on + strand at 407.082 kb on - strand at 407.140 kb on + strand, within MPMX20_00389 at 407.142 kb on - strand, within MPMX20_00389 at 407.226 kb on - strand, within MPMX20_00389 at 407.247 kb on + strand, within MPMX20_00389 at 407.305 kb on + strand, within MPMX20_00389 at 407.305 kb on + strand, within MPMX20_00389 at 407.313 kb on - strand, within MPMX20_00389 at 407.313 kb on - strand, within MPMX20_00389 at 407.313 kb on - strand, within MPMX20_00389 at 407.402 kb on - strand at 407.418 kb on - strand at 407.433 kb on - strand at 407.472 kb on - strand at 407.579 kb on - strand, within MPMX20_00390 at 407.584 kb on - strand, within MPMX20_00390 at 407.595 kb on - strand, within MPMX20_00390 at 407.627 kb on - strand, within MPMX20_00390 at 407.746 kb on + strand, within MPMX20_00390 at 407.746 kb on + strand, within MPMX20_00390 at 407.754 kb on - strand, within MPMX20_00390 at 407.754 kb on - strand, within MPMX20_00390 at 407.754 kb on - strand, within MPMX20_00390 at 407.754 kb on - strand, within MPMX20_00390
Per-strain Table
Position Strand Gene LocusTag Fraction M9 plate, 1:1 coculture with Paraburkholderia phenoluptrix CO2 remove 405,712 - +2.1 405,718 + -1.3 405,735 + +3.8 405,736 - -2.7 405,736 - +1.1 405,743 - -0.1 405,769 + MPMX20_00385 0.11 -0.5 405,861 + MPMX20_00385 0.21 -0.2 405,899 - MPMX20_00385 0.26 -0.0 405,918 + MPMX20_00385 0.28 +1.3 405,923 + MPMX20_00385 0.28 -0.6 405,940 - MPMX20_00385 0.30 +0.8 405,987 + MPMX20_00385 0.36 -0.6 406,019 + MPMX20_00385 0.40 +0.4 406,055 + MPMX20_00385 0.44 -0.9 406,125 + MPMX20_00385 0.52 +0.8 406,155 + MPMX20_00385 0.55 -1.6 406,155 + MPMX20_00385 0.55 +0.8 406,181 - MPMX20_00385 0.58 -2.8 406,203 - MPMX20_00385 0.61 -1.5 406,222 - MPMX20_00385 0.63 -2.0 406,224 + MPMX20_00385 0.63 +0.1 406,236 + MPMX20_00385 0.65 +0.6 406,327 + MPMX20_00385 0.75 -1.6 406,365 + MPMX20_00385 0.80 -1.8 406,373 - MPMX20_00385 0.81 -0.8 406,375 + MPMX20_00385 0.81 -0.8 406,375 + MPMX20_00385 0.81 +0.3 406,378 - MPMX20_00385 0.81 +1.5 406,487 + -0.5 406,487 + -1.1 406,487 + +0.3 406,495 - +0.4 406,519 + +1.1 406,534 + -1.0 406,534 + -1.5 406,534 + +0.3 406,556 + -0.5 406,564 - -1.3 406,573 - +0.7 406,601 - MPMX20_00386 0.30 +0.5 406,668 + MPMX20_00386 0.81 -2.1 406,668 + MPMX20_00386 0.81 -0.1 406,676 - MPMX20_00386 0.87 -0.7 406,676 - MPMX20_00386 0.87 +1.5 406,715 + MPMX20_00387 0.10 +1.5 406,772 - MPMX20_00387 0.61 +1.6 406,772 - MPMX20_00387 0.61 +0.8 406,772 - MPMX20_00387 0.61 +2.1 406,774 + MPMX20_00387 0.63 +3.4 406,774 + MPMX20_00387 0.63 +0.3 406,777 - MPMX20_00387 0.66 +0.0 406,782 - MPMX20_00387 0.70 -3.3 406,827 + +5.8 406,872 + -0.6 406,893 + MPMX20_00388 0.25 -0.1 406,966 + +0.5 406,976 + +2.2 406,981 + +15.6 406,981 + -0.8 406,981 + -1.6 406,981 + +1.1 406,981 + -0.7 406,984 - -0.6 406,989 - -0.8 407,052 - -2.9 407,067 + -0.2 407,074 + -1.8 407,082 - -1.1 407,140 + MPMX20_00389 0.22 +0.1 407,142 - MPMX20_00389 0.23 +0.2 407,226 - MPMX20_00389 0.44 -2.2 407,247 + MPMX20_00389 0.50 +0.5 407,305 + MPMX20_00389 0.65 +1.2 407,305 + MPMX20_00389 0.65 -0.1 407,313 - MPMX20_00389 0.67 -0.4 407,313 - MPMX20_00389 0.67 -2.5 407,313 - MPMX20_00389 0.67 -2.0 407,402 - +0.1 407,418 - +0.8 407,433 - +1.1 407,472 - -1.8 407,579 - MPMX20_00390 0.24 +1.5 407,584 - MPMX20_00390 0.25 -1.1 407,595 - MPMX20_00390 0.27 +0.5 407,627 - MPMX20_00390 0.35 -1.2 407,746 + MPMX20_00390 0.64 -2.0 407,746 + MPMX20_00390 0.64 -1.9 407,754 - MPMX20_00390 0.65 +1.5 407,754 - MPMX20_00390 0.65 +0.6 407,754 - MPMX20_00390 0.65 -1.2 407,754 - MPMX20_00390 0.65 -0.8
Or see this region's nucleotide sequence