Experiment: M9 plate, 1:1 coculture with Paraburkholderia phenoluptrix CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00094 and MPMX20_00095 are separated by 120 nucleotides MPMX20_00095 and MPMX20_00096 overlap by 23 nucleotides MPMX20_00096 and MPMX20_00097 are separated by 109 nucleotides
MPMX20_00094: MPMX20_00094 - Nucleoid occlusion factor SlmA, at 97,138 to 97,734
_00094
MPMX20_00095: MPMX20_00095 - Deoxyuridine 5'-triphosphate nucleotidohydrolase, at 97,855 to 98,313
_00095
MPMX20_00096: MPMX20_00096 - Coenzyme A biosynthesis bifunctional protein CoaBC, at 98,291 to 99,505
_00096
MPMX20_00097: MPMX20_00097 - hypothetical protein, at 99,615 to 100,340
_00097
Position (kb) 98
99
100 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 97.319 kb on + strand, within MPMX20_00094 at 97.325 kb on - strand, within MPMX20_00094 at 97.334 kb on + strand, within MPMX20_00094 at 97.356 kb on + strand, within MPMX20_00094 at 97.356 kb on + strand, within MPMX20_00094 at 97.356 kb on + strand, within MPMX20_00094 at 97.364 kb on - strand, within MPMX20_00094 at 97.364 kb on - strand, within MPMX20_00094 at 97.364 kb on - strand, within MPMX20_00094 at 97.390 kb on + strand, within MPMX20_00094 at 97.403 kb on - strand, within MPMX20_00094 at 97.410 kb on + strand, within MPMX20_00094 at 97.418 kb on - strand, within MPMX20_00094 at 97.432 kb on + strand, within MPMX20_00094 at 97.447 kb on - strand, within MPMX20_00094 at 97.626 kb on + strand, within MPMX20_00094 at 97.629 kb on + strand, within MPMX20_00094 at 97.634 kb on - strand, within MPMX20_00094 at 97.713 kb on - strand at 97.802 kb on - strand at 97.848 kb on - strand at 99.562 kb on - strand at 99.603 kb on - strand at 99.631 kb on + strand at 99.673 kb on + strand at 99.682 kb on + strand at 99.690 kb on - strand, within MPMX20_00097 at 99.690 kb on - strand, within MPMX20_00097 at 99.742 kb on - strand, within MPMX20_00097 at 99.769 kb on - strand, within MPMX20_00097 at 99.778 kb on - strand, within MPMX20_00097 at 99.780 kb on - strand, within MPMX20_00097 at 99.813 kb on - strand, within MPMX20_00097 at 99.836 kb on + strand, within MPMX20_00097 at 99.927 kb on - strand, within MPMX20_00097 at 99.967 kb on + strand, within MPMX20_00097 at 100.101 kb on + strand, within MPMX20_00097 at 100.111 kb on + strand, within MPMX20_00097 at 100.112 kb on + strand, within MPMX20_00097 at 100.112 kb on + strand, within MPMX20_00097 at 100.114 kb on - strand, within MPMX20_00097 at 100.201 kb on + strand, within MPMX20_00097 at 100.201 kb on + strand, within MPMX20_00097 at 100.206 kb on + strand, within MPMX20_00097 at 100.206 kb on + strand, within MPMX20_00097 at 100.388 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 plate, 1:1 coculture with Paraburkholderia phenoluptrix CO2 remove 97,319 + MPMX20_00094 0.30 -1.8 97,325 - MPMX20_00094 0.31 -0.5 97,334 + MPMX20_00094 0.33 -0.1 97,356 + MPMX20_00094 0.37 -1.7 97,356 + MPMX20_00094 0.37 +0.2 97,356 + MPMX20_00094 0.37 +2.1 97,364 - MPMX20_00094 0.38 -2.4 97,364 - MPMX20_00094 0.38 +1.1 97,364 - MPMX20_00094 0.38 +1.1 97,390 + MPMX20_00094 0.42 +1.1 97,403 - MPMX20_00094 0.44 -1.2 97,410 + MPMX20_00094 0.46 -1.0 97,418 - MPMX20_00094 0.47 +2.1 97,432 + MPMX20_00094 0.49 +0.0 97,447 - MPMX20_00094 0.52 +1.0 97,626 + MPMX20_00094 0.82 +0.7 97,629 + MPMX20_00094 0.82 -0.1 97,634 - MPMX20_00094 0.83 +0.4 97,713 - +0.4 97,802 - -0.0 97,848 - -0.0 99,562 - +0.5 99,603 - -0.0 99,631 + -1.8 99,673 + -0.1 99,682 + -0.4 99,690 - MPMX20_00097 0.10 -2.3 99,690 - MPMX20_00097 0.10 +1.1 99,742 - MPMX20_00097 0.17 -0.8 99,769 - MPMX20_00097 0.21 +0.7 99,778 - MPMX20_00097 0.22 -0.6 99,780 - MPMX20_00097 0.23 +4.9 99,813 - MPMX20_00097 0.27 +3.1 99,836 + MPMX20_00097 0.30 +3.1 99,927 - MPMX20_00097 0.43 +3.6 99,967 + MPMX20_00097 0.48 +0.7 100,101 + MPMX20_00097 0.67 +1.1 100,111 + MPMX20_00097 0.68 -2.2 100,112 + MPMX20_00097 0.68 +1.7 100,112 + MPMX20_00097 0.68 +1.1 100,114 - MPMX20_00097 0.69 +0.1 100,201 + MPMX20_00097 0.81 -1.8 100,201 + MPMX20_00097 0.81 +2.1 100,206 + MPMX20_00097 0.81 -0.6 100,206 + MPMX20_00097 0.81 -1.8 100,388 - -0.9
Or see this region's nucleotide sequence