Experiment: 0.1X LB plate, 1:1 coculture with Paraburkholderia pittii CO4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_03685 and MPMX20_03686 are separated by 12 nucleotides MPMX20_03686 and MPMX20_03687 overlap by 16 nucleotides MPMX20_03687 and MPMX20_03688 overlap by 4 nucleotides MPMX20_03688 and MPMX20_03689 overlap by 10 nucleotides MPMX20_03689 and MPMX20_03690 are separated by 184 nucleotides
MPMX20_03685: MPMX20_03685 - RecBCD enzyme subunit RecC, at 3,883,401 to 3,886,775
_03685
MPMX20_03686: MPMX20_03686 - hypothetical protein, at 3,886,788 to 3,887,111
_03686
MPMX20_03687: MPMX20_03687 - hypothetical protein, at 3,887,096 to 3,887,497
_03687
MPMX20_03688: MPMX20_03688 - hypothetical protein, at 3,887,494 to 3,888,054
_03688
MPMX20_03689: MPMX20_03689 - hypothetical protein, at 3,888,045 to 3,888,509
_03689
MPMX20_03690: MPMX20_03690 - Thymidylate synthase, at 3,888,694 to 3,889,488
_03690
Position (kb)
3887
3888
3889 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 3886.547 kb on - strand at 3886.814 kb on - strand at 3886.878 kb on - strand, within MPMX20_03686 at 3886.897 kb on - strand, within MPMX20_03686 at 3886.897 kb on - strand, within MPMX20_03686 at 3886.965 kb on - strand, within MPMX20_03686 at 3887.116 kb on - strand at 3887.118 kb on + strand at 3887.299 kb on - strand, within MPMX20_03687 at 3887.312 kb on + strand, within MPMX20_03687 at 3887.344 kb on + strand, within MPMX20_03687 at 3887.389 kb on - strand, within MPMX20_03687 at 3887.553 kb on + strand, within MPMX20_03688 at 3887.561 kb on - strand, within MPMX20_03688 at 3887.614 kb on - strand, within MPMX20_03688 at 3887.616 kb on + strand, within MPMX20_03688 at 3887.641 kb on + strand, within MPMX20_03688 at 3887.649 kb on - strand, within MPMX20_03688 at 3887.665 kb on + strand, within MPMX20_03688 at 3887.665 kb on + strand, within MPMX20_03688 at 3887.666 kb on + strand, within MPMX20_03688 at 3887.666 kb on + strand, within MPMX20_03688 at 3887.709 kb on - strand, within MPMX20_03688 at 3887.735 kb on - strand, within MPMX20_03688 at 3887.766 kb on - strand, within MPMX20_03688 at 3887.826 kb on + strand, within MPMX20_03688 at 3887.826 kb on + strand, within MPMX20_03688 at 3887.849 kb on + strand, within MPMX20_03688 at 3887.874 kb on - strand, within MPMX20_03688 at 3887.923 kb on - strand, within MPMX20_03688 at 3887.993 kb on + strand, within MPMX20_03688 at 3887.993 kb on - strand, within MPMX20_03688 at 3888.003 kb on - strand at 3888.035 kb on - strand at 3888.042 kb on - strand at 3888.092 kb on - strand, within MPMX20_03689 at 3888.130 kb on - strand, within MPMX20_03689 at 3888.141 kb on + strand, within MPMX20_03689 at 3888.246 kb on + strand, within MPMX20_03689 at 3888.246 kb on + strand, within MPMX20_03689 at 3888.266 kb on - strand, within MPMX20_03689 at 3888.266 kb on - strand, within MPMX20_03689 at 3888.285 kb on + strand, within MPMX20_03689 at 3888.293 kb on - strand, within MPMX20_03689 at 3888.293 kb on - strand, within MPMX20_03689 at 3888.293 kb on - strand, within MPMX20_03689 at 3888.320 kb on + strand, within MPMX20_03689 at 3888.325 kb on + strand, within MPMX20_03689 at 3888.333 kb on - strand, within MPMX20_03689 at 3888.367 kb on + strand, within MPMX20_03689 at 3888.385 kb on - strand, within MPMX20_03689 at 3888.431 kb on + strand, within MPMX20_03689 at 3888.485 kb on + strand at 3888.599 kb on - strand at 3888.691 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 0.1X LB plate, 1:1 coculture with Paraburkholderia pittii CO4 remove 3,886,547 - +2.6 3,886,814 - -0.1 3,886,878 - MPMX20_03686 0.28 -0.4 3,886,897 - MPMX20_03686 0.34 -0.9 3,886,897 - MPMX20_03686 0.34 +2.7 3,886,965 - MPMX20_03686 0.55 +0.2 3,887,116 - -0.1 3,887,118 + -0.4 3,887,299 - MPMX20_03687 0.50 -0.3 3,887,312 + MPMX20_03687 0.54 +0.6 3,887,344 + MPMX20_03687 0.62 +1.0 3,887,389 - MPMX20_03687 0.73 -1.5 3,887,553 + MPMX20_03688 0.11 +0.6 3,887,561 - MPMX20_03688 0.12 +2.6 3,887,614 - MPMX20_03688 0.21 -0.1 3,887,616 + MPMX20_03688 0.22 +2.9 3,887,641 + MPMX20_03688 0.26 -1.4 3,887,649 - MPMX20_03688 0.28 +0.5 3,887,665 + MPMX20_03688 0.30 -2.8 3,887,665 + MPMX20_03688 0.30 +0.8 3,887,666 + MPMX20_03688 0.31 -1.8 3,887,666 + MPMX20_03688 0.31 -2.3 3,887,709 - MPMX20_03688 0.38 -0.7 3,887,735 - MPMX20_03688 0.43 -0.9 3,887,766 - MPMX20_03688 0.48 -0.5 3,887,826 + MPMX20_03688 0.59 -1.3 3,887,826 + MPMX20_03688 0.59 -1.1 3,887,849 + MPMX20_03688 0.63 -0.4 3,887,874 - MPMX20_03688 0.68 -0.8 3,887,923 - MPMX20_03688 0.76 +0.4 3,887,993 + MPMX20_03688 0.89 -2.1 3,887,993 - MPMX20_03688 0.89 +3.0 3,888,003 - -1.9 3,888,035 - +0.2 3,888,042 - -0.9 3,888,092 - MPMX20_03689 0.10 +0.2 3,888,130 - MPMX20_03689 0.18 +0.5 3,888,141 + MPMX20_03689 0.21 -1.2 3,888,246 + MPMX20_03689 0.43 -2.8 3,888,246 + MPMX20_03689 0.43 +0.0 3,888,266 - MPMX20_03689 0.48 -0.7 3,888,266 - MPMX20_03689 0.48 -0.0 3,888,285 + MPMX20_03689 0.52 -0.4 3,888,293 - MPMX20_03689 0.53 -0.1 3,888,293 - MPMX20_03689 0.53 +0.9 3,888,293 - MPMX20_03689 0.53 +5.0 3,888,320 + MPMX20_03689 0.59 -0.4 3,888,325 + MPMX20_03689 0.60 -0.4 3,888,333 - MPMX20_03689 0.62 +1.8 3,888,367 + MPMX20_03689 0.69 +0.0 3,888,385 - MPMX20_03689 0.73 -0.1 3,888,431 + MPMX20_03689 0.83 -1.4 3,888,485 + +1.6 3,888,599 - +0.1 3,888,691 + -1.9
Or see this region's nucleotide sequence