Experiment: monoculture; Experiment C, time point 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_322 and HP15_323 are separated by 22 nucleotides HP15_323 and HP15_324 overlap by 4 nucleotides HP15_324 and HP15_325 are separated by 59 nucleotides HP15_325 and HP15_326 overlap by 4 nucleotides
HP15_322: HP15_322 - glucose-6-phosphate isomerase, at 318,318 to 319,979
_322
HP15_323: HP15_323 - pantothenate synthetase, at 320,002 to 320,850
_323
HP15_324: HP15_324 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 320,847 to 321,641
_324
HP15_325: HP15_325 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase, at 321,701 to 322,198
_325
HP15_326: HP15_326 - polyA polymerase, at 322,195 to 323,526
_326
Position (kb) 320
321
322 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 319.985 kb on - strand at 321.670 kb on - strand at 322.261 kb on - strand at 322.273 kb on - strand at 322.293 kb on - strand at 322.382 kb on + strand, within HP15_326 at 322.562 kb on - strand, within HP15_326 at 322.573 kb on - strand, within HP15_326 at 322.577 kb on - strand, within HP15_326 at 322.604 kb on + strand, within HP15_326
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment C, time point 4 remove 319,985 - -1.3 321,670 - +2.3 322,261 - -0.7 322,273 - -0.7 322,293 - +1.9 322,382 + HP15_326 0.14 -4.0 322,562 - HP15_326 0.28 -2.3 322,573 - HP15_326 0.28 -1.1 322,577 - HP15_326 0.29 -2.2 322,604 + HP15_326 0.31 -3.2
Or see this region's nucleotide sequence