Experiment: monoculture; Experiment C, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_539 and HP15_540 overlap by 1 nucleotides HP15_540 and HP15_541 are separated by 43 nucleotides HP15_541 and HP15_542 are separated by 21 nucleotides
HP15_539: HP15_539 - 2-isopropylmalate synthase, at 561,614 to 562,372
_539
HP15_540: HP15_540 - ribosomal-protein-alanine acetyltransferase, at 562,372 to 562,857
_540
HP15_541: HP15_541 - peptide chain release factor 3, at 562,901 to 564,526
_541
HP15_542: HP15_542 - TatD-related deoxyribonuclease, at 564,548 to 565,333
_542
Position (kb) 562
563
564
565 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 562.445 kb on + strand, within HP15_540 at 562.533 kb on + strand, within HP15_540 at 562.533 kb on + strand, within HP15_540 at 562.533 kb on + strand, within HP15_540 at 562.767 kb on - strand, within HP15_540 at 562.787 kb on + strand, within HP15_540 at 562.856 kb on + strand at 562.955 kb on - strand at 563.017 kb on + strand at 563.167 kb on - strand, within HP15_541 at 563.260 kb on - strand, within HP15_541 at 563.292 kb on - strand, within HP15_541 at 563.300 kb on - strand, within HP15_541 at 563.525 kb on + strand, within HP15_541 at 563.635 kb on + strand, within HP15_541 at 563.643 kb on - strand, within HP15_541 at 563.643 kb on - strand, within HP15_541 at 563.643 kb on - strand, within HP15_541 at 563.885 kb on - strand, within HP15_541 at 564.070 kb on - strand, within HP15_541 at 564.311 kb on + strand, within HP15_541 at 564.362 kb on - strand, within HP15_541 at 564.457 kb on - strand at 564.646 kb on - strand, within HP15_542 at 564.674 kb on + strand, within HP15_542 at 564.674 kb on + strand, within HP15_542 at 564.718 kb on + strand, within HP15_542 at 564.739 kb on + strand, within HP15_542 at 564.936 kb on - strand, within HP15_542 at 565.152 kb on - strand, within HP15_542 at 565.251 kb on - strand, within HP15_542
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment C, time point 2 remove 562,445 + HP15_540 0.15 -0.2 562,533 + HP15_540 0.33 -0.2 562,533 + HP15_540 0.33 +0.2 562,533 + HP15_540 0.33 -0.7 562,767 - HP15_540 0.81 -0.1 562,787 + HP15_540 0.85 +0.1 562,856 + +0.6 562,955 - -1.6 563,017 + -0.5 563,167 - HP15_541 0.16 -1.4 563,260 - HP15_541 0.22 -4.2 563,292 - HP15_541 0.24 -1.0 563,300 - HP15_541 0.25 -0.2 563,525 + HP15_541 0.38 -2.1 563,635 + HP15_541 0.45 -0.7 563,643 - HP15_541 0.46 +0.1 563,643 - HP15_541 0.46 -0.5 563,643 - HP15_541 0.46 -2.6 563,885 - HP15_541 0.61 -1.2 564,070 - HP15_541 0.72 -1.3 564,311 + HP15_541 0.87 +1.0 564,362 - HP15_541 0.90 -0.3 564,457 - +0.6 564,646 - HP15_542 0.12 +0.4 564,674 + HP15_542 0.16 -0.6 564,674 + HP15_542 0.16 +0.7 564,718 + HP15_542 0.22 -1.1 564,739 + HP15_542 0.24 -0.7 564,936 - HP15_542 0.49 +0.3 565,152 - HP15_542 0.77 -0.4 565,251 - HP15_542 0.89 -0.8
Or see this region's nucleotide sequence