Experiment: monoculture; Experiment C, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_425 and HP15_426 overlap by 4 nucleotides HP15_426 and HP15_427 are separated by 254 nucleotides HP15_427 and HP15_428 are separated by 173 nucleotides
HP15_425: HP15_425 - two-component response regulator NtrC, at 422,734 to 424,161
_425
HP15_426: HP15_426 - signal transduction histidine kinase, nitrogen specific, NtrB, at 424,158 to 425,252
_426
HP15_427: HP15_427 - conserved hypothetical protein, secreted, at 425,507 to 426,031
_427
HP15_428: HP15_428 - glutamine synthetase, type I, at 426,205 to 427,608
_428
Position (kb)
424
425
426 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 423.212 kb on + strand, within HP15_425 at 423.229 kb on - strand, within HP15_425 at 423.230 kb on - strand, within HP15_425 at 423.406 kb on - strand, within HP15_425 at 423.565 kb on + strand, within HP15_425 at 423.565 kb on + strand, within HP15_425 at 423.573 kb on - strand, within HP15_425 at 423.573 kb on - strand, within HP15_425 at 423.809 kb on + strand, within HP15_425 at 423.827 kb on - strand, within HP15_425 at 423.856 kb on + strand, within HP15_425 at 423.903 kb on - strand, within HP15_425 at 424.081 kb on + strand at 424.086 kb on + strand at 424.086 kb on + strand at 424.086 kb on + strand at 424.094 kb on - strand at 424.094 kb on - strand at 424.094 kb on - strand at 424.094 kb on - strand at 424.094 kb on - strand at 424.099 kb on + strand at 424.107 kb on - strand at 424.107 kb on - strand at 424.107 kb on - strand at 424.144 kb on - strand at 424.144 kb on - strand at 424.167 kb on - strand at 424.236 kb on + strand at 424.236 kb on + strand at 424.251 kb on + strand at 424.251 kb on + strand at 424.258 kb on + strand at 424.277 kb on - strand, within HP15_426 at 424.382 kb on - strand, within HP15_426 at 424.407 kb on + strand, within HP15_426 at 424.417 kb on + strand, within HP15_426 at 424.423 kb on - strand, within HP15_426 at 424.456 kb on - strand, within HP15_426 at 424.487 kb on + strand, within HP15_426 at 424.529 kb on + strand, within HP15_426 at 424.540 kb on + strand, within HP15_426 at 424.540 kb on + strand, within HP15_426 at 424.621 kb on - strand, within HP15_426 at 424.666 kb on + strand, within HP15_426 at 424.674 kb on - strand, within HP15_426 at 424.778 kb on + strand, within HP15_426 at 424.976 kb on + strand, within HP15_426 at 425.017 kb on + strand, within HP15_426 at 425.154 kb on + strand at 425.189 kb on - strand at 425.213 kb on - strand at 425.407 kb on + strand at 425.407 kb on + strand at 425.415 kb on - strand at 425.473 kb on + strand at 425.518 kb on - strand at 425.704 kb on + strand, within HP15_427 at 425.704 kb on + strand, within HP15_427 at 425.712 kb on - strand, within HP15_427 at 425.851 kb on + strand, within HP15_427 at 425.851 kb on + strand, within HP15_427 at 425.851 kb on + strand, within HP15_427 at 425.856 kb on + strand, within HP15_427 at 425.864 kb on - strand, within HP15_427 at 425.866 kb on + strand, within HP15_427 at 425.883 kb on + strand, within HP15_427 at 425.920 kb on + strand, within HP15_427 at 425.928 kb on - strand, within HP15_427 at 425.928 kb on - strand, within HP15_427 at 425.966 kb on - strand, within HP15_427 at 426.167 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment C, time point 2 remove 423,212 + HP15_425 0.33 -0.5 423,229 - HP15_425 0.35 -1.1 423,230 - HP15_425 0.35 -0.7 423,406 - HP15_425 0.47 +0.4 423,565 + HP15_425 0.58 +0.7 423,565 + HP15_425 0.58 -0.3 423,573 - HP15_425 0.59 -0.5 423,573 - HP15_425 0.59 -0.6 423,809 + HP15_425 0.75 -1.3 423,827 - HP15_425 0.77 -0.6 423,856 + HP15_425 0.79 -0.9 423,903 - HP15_425 0.82 -1.7 424,081 + -3.1 424,086 + -2.3 424,086 + +0.5 424,086 + -1.6 424,094 - -0.4 424,094 - -0.4 424,094 - +0.7 424,094 - -0.5 424,094 - -0.9 424,099 + -0.3 424,107 - +0.7 424,107 - -0.5 424,107 - -0.3 424,144 - -0.5 424,144 - -1.0 424,167 - -0.6 424,236 + -0.3 424,236 + -0.1 424,251 + -0.7 424,251 + -0.3 424,258 + -1.1 424,277 - HP15_426 0.11 -0.0 424,382 - HP15_426 0.20 -0.3 424,407 + HP15_426 0.23 -1.1 424,417 + HP15_426 0.24 -1.4 424,423 - HP15_426 0.24 -1.8 424,456 - HP15_426 0.27 +0.3 424,487 + HP15_426 0.30 -0.9 424,529 + HP15_426 0.34 -1.8 424,540 + HP15_426 0.35 -0.5 424,540 + HP15_426 0.35 -0.7 424,621 - HP15_426 0.42 +0.1 424,666 + HP15_426 0.46 -0.7 424,674 - HP15_426 0.47 -1.5 424,778 + HP15_426 0.57 -1.9 424,976 + HP15_426 0.75 -0.3 425,017 + HP15_426 0.78 -1.1 425,154 + -1.5 425,189 - -0.3 425,213 - +0.1 425,407 + +0.6 425,407 + -0.6 425,415 - +0.4 425,473 + -0.6 425,518 - -1.0 425,704 + HP15_427 0.38 +0.5 425,704 + HP15_427 0.38 +0.4 425,712 - HP15_427 0.39 +0.3 425,851 + HP15_427 0.66 +0.2 425,851 + HP15_427 0.66 +0.3 425,851 + HP15_427 0.66 -0.2 425,856 + HP15_427 0.66 +0.2 425,864 - HP15_427 0.68 -0.3 425,866 + HP15_427 0.68 +0.8 425,883 + HP15_427 0.72 +1.0 425,920 + HP15_427 0.79 +0.1 425,928 - HP15_427 0.80 -0.1 425,928 - HP15_427 0.80 -1.8 425,966 - HP15_427 0.87 -0.3 426,167 + -1.1
Or see this region's nucleotide sequence