Experiment: monoculture; Experiment C, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_420 and HP15_421 are separated by 26 nucleotides HP15_421 and HP15_422 overlap by 1 nucleotides HP15_422 and HP15_423 overlap by 11 nucleotides
HP15_420: HP15_420 - type II secretory pathway, pseudopilin PulG, at 416,110 to 416,577
_420
HP15_421: HP15_421 - MSHA pilin protein MshD, at 416,604 to 417,062
_421
HP15_422: HP15_422 - protein containing prepilin-type cleavage/methylation, N-terminal domains, at 417,062 to 417,847
_422
HP15_423: HP15_423 - conserved hypothetical protein, at 417,837 to 418,250
_423
Position (kb) 416
417
418 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 415.635 kb on - strand at 415.731 kb on + strand at 415.887 kb on + strand at 415.889 kb on + strand at 415.927 kb on - strand at 415.960 kb on - strand at 416.075 kb on + strand at 416.227 kb on + strand, within HP15_420 at 416.399 kb on - strand, within HP15_420 at 416.511 kb on + strand, within HP15_420 at 416.591 kb on - strand at 416.708 kb on - strand, within HP15_421 at 416.729 kb on + strand, within HP15_421 at 416.729 kb on + strand, within HP15_421 at 416.912 kb on - strand, within HP15_421 at 416.940 kb on + strand, within HP15_421 at 416.940 kb on + strand, within HP15_421 at 417.078 kb on + strand at 417.168 kb on + strand, within HP15_422 at 417.233 kb on + strand, within HP15_422 at 417.242 kb on + strand, within HP15_422 at 417.242 kb on + strand, within HP15_422 at 417.299 kb on - strand, within HP15_422 at 417.299 kb on - strand, within HP15_422 at 417.327 kb on + strand, within HP15_422 at 417.421 kb on - strand, within HP15_422 at 417.434 kb on - strand, within HP15_422 at 417.489 kb on - strand, within HP15_422 at 417.750 kb on - strand, within HP15_422 at 417.771 kb on + strand at 417.771 kb on + strand at 417.841 kb on + strand at 417.849 kb on - strand at 417.874 kb on + strand at 418.005 kb on + strand, within HP15_423
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment C, time point 2 remove 415,635 - -0.8 415,731 + -0.1 415,887 + -0.6 415,889 + -0.7 415,927 - +0.5 415,960 - -0.1 416,075 + +0.3 416,227 + HP15_420 0.25 -1.0 416,399 - HP15_420 0.62 +0.9 416,511 + HP15_420 0.86 -1.6 416,591 - -1.2 416,708 - HP15_421 0.23 +0.4 416,729 + HP15_421 0.27 +0.7 416,729 + HP15_421 0.27 +0.8 416,912 - HP15_421 0.67 -0.4 416,940 + HP15_421 0.73 +0.4 416,940 + HP15_421 0.73 +1.4 417,078 + -0.4 417,168 + HP15_422 0.13 -0.4 417,233 + HP15_422 0.22 +0.8 417,242 + HP15_422 0.23 -2.9 417,242 + HP15_422 0.23 -0.4 417,299 - HP15_422 0.30 +0.4 417,299 - HP15_422 0.30 -2.4 417,327 + HP15_422 0.34 -0.7 417,421 - HP15_422 0.46 +0.2 417,434 - HP15_422 0.47 +0.0 417,489 - HP15_422 0.54 +0.4 417,750 - HP15_422 0.88 -0.1 417,771 + -0.4 417,771 + +0.6 417,841 + -0.8 417,849 - +0.0 417,874 + -0.8 418,005 + HP15_423 0.41 -0.2
Or see this region's nucleotide sequence