Experiment: monoculture; Experiment C, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_2440 and HP15_2441 overlap by 23 nucleotides HP15_2441 and HP15_2442 are separated by 150 nucleotides HP15_2442 and HP15_2443 are separated by 100 nucleotides HP15_2443 and HP15_2444 are separated by 87 nucleotides HP15_2444 and HP15_2445 are separated by 22 nucleotides
HP15_2440: HP15_2440 - OstA family protein, at 2,578,484 to 2,579,050
_2440
HP15_2441: HP15_2441 - protein containing ABC transporter-like domain, at 2,579,028 to 2,579,753
_2441
HP15_2442: HP15_2442 - RNA polymerase, sigma 54 subunit, RpoN, at 2,579,904 to 2,581,430
_2442
HP15_2443: HP15_2443 - sigma 54 modulation protein/ribosomal protein S30EA, at 2,581,531 to 2,581,839
_2443
HP15_2444: HP15_2444 - PTS IIA-like nitrogen-regulatory protein PtsN, at 2,581,927 to 2,582,397
_2444
HP15_2445: HP15_2445 - protein yhbJ, at 2,582,420 to 2,583,310
_2445
Position (kb)
2579
2580
2581
2582 Strain fitness (log2 ratio)
-2
-1
0
1 at 2580.063 kb on - strand, within HP15_2442 at 2580.401 kb on - strand, within HP15_2442 at 2580.510 kb on - strand, within HP15_2442 at 2580.635 kb on - strand, within HP15_2442 at 2580.665 kb on + strand, within HP15_2442 at 2580.920 kb on - strand, within HP15_2442 at 2581.016 kb on + strand, within HP15_2442 at 2581.016 kb on + strand, within HP15_2442 at 2581.386 kb on + strand at 2581.452 kb on - strand at 2581.556 kb on + strand at 2581.710 kb on + strand, within HP15_2443
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment C, time point 2 remove 2,580,063 - HP15_2442 0.10 -1.9 2,580,401 - HP15_2442 0.33 -0.8 2,580,510 - HP15_2442 0.40 -1.5 2,580,635 - HP15_2442 0.48 -0.6 2,580,665 + HP15_2442 0.50 -1.8 2,580,920 - HP15_2442 0.67 -2.2 2,581,016 + HP15_2442 0.73 -1.8 2,581,016 + HP15_2442 0.73 +1.6 2,581,386 + -1.4 2,581,452 - -2.5 2,581,556 + -0.8 2,581,710 + HP15_2443 0.58 -0.6
Or see this region's nucleotide sequence