Experiment: monoculture; Experiment C, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_210 and HP15_211 are separated by 11 nucleotides HP15_211 and HP15_212 overlap by 25 nucleotides HP15_212 and HP15_213 are separated by 146 nucleotides HP15_213 and HP15_214 are separated by 64 nucleotides
HP15_210: HP15_210 - phospholipase A(1), at 197,198 to 198,325
_210
HP15_211: HP15_211 - conserved hypothetical protein, membrane, at 198,337 to 198,675
_211
HP15_212: HP15_212 - hypothetical protein, at 198,651 to 198,788
_212
HP15_213: HP15_213 - cytochrome c5, at 198,935 to 199,291
_213
HP15_214: HP15_214 - DNA helicase, ATP-dependent, RepA type, at 199,356 to 201,374
_214
Position (kb) 198
199
200 Strain fitness (log2 ratio) -1
0
1 at 198.011 kb on - strand, within HP15_210 at 198.029 kb on + strand, within HP15_210 at 198.107 kb on - strand, within HP15_210 at 198.123 kb on + strand, within HP15_210 at 198.137 kb on - strand, within HP15_210 at 198.203 kb on + strand, within HP15_210 at 198.222 kb on - strand at 198.359 kb on - strand at 198.403 kb on + strand, within HP15_211 at 198.609 kb on - strand, within HP15_211 at 198.748 kb on - strand, within HP15_212 at 198.813 kb on + strand at 198.821 kb on - strand at 198.885 kb on - strand at 198.909 kb on + strand at 199.022 kb on + strand, within HP15_213 at 199.041 kb on + strand, within HP15_213 at 199.148 kb on + strand, within HP15_213 at 199.258 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment C, time point 2 remove 198,011 - HP15_210 0.72 +0.7 198,029 + HP15_210 0.74 +0.1 198,107 - HP15_210 0.81 -0.2 198,123 + HP15_210 0.82 +0.9 198,137 - HP15_210 0.83 +1.1 198,203 + HP15_210 0.89 +0.1 198,222 - -0.0 198,359 - +0.0 198,403 + HP15_211 0.19 -0.1 198,609 - HP15_211 0.80 -0.1 198,748 - HP15_212 0.70 +0.5 198,813 + -0.7 198,821 - -0.2 198,885 - -1.5 198,909 + +0.1 199,022 + HP15_213 0.24 -0.4 199,041 + HP15_213 0.30 +1.7 199,148 + HP15_213 0.60 -1.6 199,258 - -0.6
Or see this region's nucleotide sequence