Experiment: monoculture; Experiment C, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_125 and HP15_126 are separated by 68 nucleotides HP15_126 and HP15_127 are separated by 27 nucleotides HP15_127 and HP15_128 overlap by 14 nucleotides HP15_128 and HP15_129 are separated by 57 nucleotides
HP15_125: HP15_125 - conserved hypothetical protein, at 134,272 to 134,613
_125
HP15_126: HP15_126 - single-strand DNA-binding protein, at 134,682 to 135,080
_126
HP15_127: HP15_127 - hypothetical protein, at 135,108 to 135,353
_127
HP15_128: HP15_128 - conserved hypothetical protein, at 135,340 to 135,660
_128
HP15_129: HP15_129 - RecT protein, at 135,718 to 136,674
_129
Position (kb) 135
136 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 134.409 kb on - strand, within HP15_125 at 134.421 kb on - strand, within HP15_125 at 134.476 kb on - strand, within HP15_125 at 134.673 kb on - strand at 134.733 kb on + strand, within HP15_126 at 134.791 kb on - strand, within HP15_126 at 134.791 kb on - strand, within HP15_126 at 134.820 kb on - strand, within HP15_126 at 134.881 kb on + strand, within HP15_126 at 134.896 kb on - strand, within HP15_126 at 134.939 kb on - strand, within HP15_126 at 134.986 kb on + strand, within HP15_126 at 135.096 kb on + strand at 135.216 kb on - strand, within HP15_127 at 135.278 kb on + strand, within HP15_127 at 135.399 kb on + strand, within HP15_128 at 135.402 kb on - strand, within HP15_128 at 135.435 kb on + strand, within HP15_128 at 135.435 kb on + strand, within HP15_128 at 135.504 kb on + strand, within HP15_128 at 135.505 kb on - strand, within HP15_128 at 135.526 kb on + strand, within HP15_128 at 135.559 kb on - strand, within HP15_128 at 135.569 kb on + strand, within HP15_128 at 135.577 kb on - strand, within HP15_128 at 135.633 kb on - strand at 135.641 kb on - strand at 135.662 kb on + strand at 135.720 kb on - strand at 135.739 kb on + strand at 135.757 kb on + strand at 135.757 kb on + strand at 135.851 kb on + strand, within HP15_129 at 135.909 kb on - strand, within HP15_129 at 135.993 kb on + strand, within HP15_129 at 136.067 kb on - strand, within HP15_129 at 136.129 kb on + strand, within HP15_129 at 136.148 kb on + strand, within HP15_129 at 136.404 kb on + strand, within HP15_129 at 136.409 kb on + strand, within HP15_129 at 136.501 kb on + strand, within HP15_129 at 136.501 kb on - strand, within HP15_129 at 136.506 kb on - strand, within HP15_129 at 136.509 kb on - strand, within HP15_129 at 136.509 kb on - strand, within HP15_129 at 136.511 kb on - strand, within HP15_129 at 136.511 kb on - strand, within HP15_129 at 136.511 kb on - strand, within HP15_129 at 136.600 kb on + strand at 136.605 kb on + strand at 136.613 kb on - strand at 136.615 kb on - strand at 136.626 kb on + strand at 136.646 kb on - strand at 136.651 kb on - strand at 136.656 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment C, time point 2 remove 134,409 - HP15_125 0.40 +0.6 134,421 - HP15_125 0.44 -0.1 134,476 - HP15_125 0.60 -0.5 134,673 - -1.8 134,733 + HP15_126 0.13 +0.3 134,791 - HP15_126 0.27 +0.0 134,791 - HP15_126 0.27 +0.6 134,820 - HP15_126 0.35 -1.4 134,881 + HP15_126 0.50 +0.1 134,896 - HP15_126 0.54 +0.7 134,939 - HP15_126 0.64 +1.0 134,986 + HP15_126 0.76 -0.1 135,096 + -0.0 135,216 - HP15_127 0.44 -1.7 135,278 + HP15_127 0.69 +0.0 135,399 + HP15_128 0.18 +1.1 135,402 - HP15_128 0.19 +0.4 135,435 + HP15_128 0.30 +0.2 135,435 + HP15_128 0.30 +1.5 135,504 + HP15_128 0.51 -1.5 135,505 - HP15_128 0.51 +1.0 135,526 + HP15_128 0.58 +0.8 135,559 - HP15_128 0.68 -2.2 135,569 + HP15_128 0.71 +0.9 135,577 - HP15_128 0.74 -0.4 135,633 - +0.1 135,641 - +0.1 135,662 + +0.1 135,720 - -0.7 135,739 + +0.1 135,757 + +0.2 135,757 + -0.5 135,851 + HP15_129 0.14 -0.9 135,909 - HP15_129 0.20 -0.1 135,993 + HP15_129 0.29 +0.0 136,067 - HP15_129 0.36 +0.2 136,129 + HP15_129 0.43 +1.7 136,148 + HP15_129 0.45 -0.1 136,404 + HP15_129 0.72 +0.2 136,409 + HP15_129 0.72 +1.1 136,501 + HP15_129 0.82 +0.4 136,501 - HP15_129 0.82 +0.1 136,506 - HP15_129 0.82 +1.7 136,509 - HP15_129 0.83 +0.3 136,509 - HP15_129 0.83 -3.1 136,511 - HP15_129 0.83 +0.8 136,511 - HP15_129 0.83 +0.2 136,511 - HP15_129 0.83 +0.4 136,600 + +0.8 136,605 + +1.0 136,613 - -0.2 136,615 - +1.1 136,626 + -0.8 136,646 - +0.7 136,651 - -0.1 136,656 - -0.0
Or see this region's nucleotide sequence