Strain Fitness in Marinobacter adhaerens HP15 around HP15_322

Experiment: monoculture; Experiment B, time point 7

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntHP15_320 and HP15_321 overlap by 4 nucleotidesHP15_321 and HP15_322 overlap by 18 nucleotidesHP15_322 and HP15_323 are separated by 22 nucleotidesHP15_323 and HP15_324 overlap by 4 nucleotides HP15_320: HP15_320 - dihydroneopterin aldolase, at 317,410 to 317,811 _320 HP15_321: HP15_321 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase, at 317,808 to 318,335 _321 HP15_322: HP15_322 - glucose-6-phosphate isomerase, at 318,318 to 319,979 _322 HP15_323: HP15_323 - pantothenate synthetase, at 320,002 to 320,850 _323 HP15_324: HP15_324 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 320,847 to 321,641 _324 Position (kb) 318 319 320Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 317.390 kb on + strandat 317.803 kb on + strandat 317.878 kb on - strand, within HP15_321at 317.928 kb on + strand, within HP15_321at 318.116 kb on - strand, within HP15_321at 318.278 kb on + strand, within HP15_321at 318.291 kb on + strandat 319.985 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 7
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317,390 + +1.4
317,803 + -2.9
317,878 - HP15_321 0.13 -5.6
317,928 + HP15_321 0.23 -4.2
318,116 - HP15_321 0.58 -3.1
318,278 + HP15_321 0.89 -2.1
318,291 + -0.6
319,985 - -2.0

Or see this region's nucleotide sequence