Experiment: monoculture; Experiment B, time point 7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_2081 and HP15_2082 are separated by 429 nucleotides HP15_2082 and HP15_2083 are separated by 66 nucleotides HP15_2083 and HP15_2084 are separated by 57 nucleotides HP15_2084 and HP15_2085 are separated by 20 nucleotides
HP15_2081: HP15_2081 - ATP-dependent Clp protease adaptor protein ClpS, at 2,181,513 to 2,181,749
_2081
HP15_2082: HP15_2082 - cold-shock DNA-binding domain protein, at 2,182,179 to 2,182,490
_2082
HP15_2083: HP15_2083 - hypothetical protein, at 2,182,557 to 2,182,724
_2083
HP15_2084: HP15_2084 - RNA pseudouridylate synthase, at 2,182,782 to 2,183,309
_2084
HP15_2085: HP15_2085 - MutT/nudix family protein, at 2,183,330 to 2,183,770
_2085
Position (kb)
2182
2183 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2181.559 kb on + strand, within HP15_2081 at 2181.562 kb on + strand, within HP15_2081 at 2181.614 kb on - strand, within HP15_2081 at 2181.614 kb on - strand, within HP15_2081 at 2181.643 kb on - strand, within HP15_2081 at 2181.643 kb on - strand, within HP15_2081 at 2181.748 kb on + strand at 2181.801 kb on - strand at 2182.316 kb on + strand, within HP15_2082 at 2182.316 kb on + strand, within HP15_2082 at 2182.316 kb on + strand, within HP15_2082 at 2182.324 kb on - strand, within HP15_2082 at 2182.370 kb on - strand, within HP15_2082 at 2182.479 kb on + strand at 2182.487 kb on - strand at 2182.497 kb on - strand at 2182.589 kb on - strand, within HP15_2083 at 2182.631 kb on + strand, within HP15_2083 at 2182.631 kb on + strand, within HP15_2083 at 2182.639 kb on - strand, within HP15_2083 at 2182.641 kb on + strand, within HP15_2083 at 2182.649 kb on - strand, within HP15_2083 at 2182.650 kb on - strand, within HP15_2083 at 2182.663 kb on + strand, within HP15_2083 at 2182.681 kb on - strand, within HP15_2083 at 2182.762 kb on + strand at 2182.850 kb on - strand, within HP15_2084 at 2182.861 kb on + strand, within HP15_2084 at 2182.869 kb on - strand, within HP15_2084 at 2182.886 kb on - strand, within HP15_2084 at 2182.898 kb on + strand, within HP15_2084 at 2182.973 kb on + strand, within HP15_2084 at 2182.981 kb on - strand, within HP15_2084 at 2183.098 kb on - strand, within HP15_2084 at 2183.318 kb on + strand at 2183.326 kb on - strand at 2183.355 kb on - strand at 2183.432 kb on + strand, within HP15_2085 at 2183.466 kb on + strand, within HP15_2085 at 2183.474 kb on - strand, within HP15_2085 at 2183.503 kb on - strand, within HP15_2085 at 2183.541 kb on + strand, within HP15_2085 at 2183.568 kb on - strand, within HP15_2085 at 2183.586 kb on + strand, within HP15_2085 at 2183.636 kb on - strand, within HP15_2085 at 2183.647 kb on - strand, within HP15_2085 at 2183.673 kb on + strand, within HP15_2085 at 2183.681 kb on - strand, within HP15_2085
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 7 remove 2,181,559 + HP15_2081 0.19 +1.0 2,181,562 + HP15_2081 0.21 -3.1 2,181,614 - HP15_2081 0.43 -1.5 2,181,614 - HP15_2081 0.43 +1.4 2,181,643 - HP15_2081 0.55 -1.6 2,181,643 - HP15_2081 0.55 -1.6 2,181,748 + -1.7 2,181,801 - +0.5 2,182,316 + HP15_2082 0.44 -0.6 2,182,316 + HP15_2082 0.44 -0.4 2,182,316 + HP15_2082 0.44 -3.2 2,182,324 - HP15_2082 0.46 -1.3 2,182,370 - HP15_2082 0.61 -0.8 2,182,479 + +0.0 2,182,487 - -0.2 2,182,497 - -0.4 2,182,589 - HP15_2083 0.19 -0.0 2,182,631 + HP15_2083 0.44 -0.6 2,182,631 + HP15_2083 0.44 +1.6 2,182,639 - HP15_2083 0.49 +3.1 2,182,641 + HP15_2083 0.50 -0.3 2,182,649 - HP15_2083 0.55 +1.1 2,182,650 - HP15_2083 0.55 -0.1 2,182,663 + HP15_2083 0.63 -0.5 2,182,681 - HP15_2083 0.74 +1.8 2,182,762 + -1.4 2,182,850 - HP15_2084 0.13 -0.6 2,182,861 + HP15_2084 0.15 +2.0 2,182,869 - HP15_2084 0.16 -1.4 2,182,886 - HP15_2084 0.20 -1.7 2,182,898 + HP15_2084 0.22 +2.1 2,182,973 + HP15_2084 0.36 +1.6 2,182,981 - HP15_2084 0.38 -0.2 2,183,098 - HP15_2084 0.60 -1.7 2,183,318 + +1.7 2,183,326 - -0.5 2,183,355 - -1.0 2,183,432 + HP15_2085 0.23 -1.5 2,183,466 + HP15_2085 0.31 -0.2 2,183,474 - HP15_2085 0.33 -0.8 2,183,503 - HP15_2085 0.39 +1.4 2,183,541 + HP15_2085 0.48 -0.6 2,183,568 - HP15_2085 0.54 -0.4 2,183,586 + HP15_2085 0.58 +2.0 2,183,636 - HP15_2085 0.69 -0.5 2,183,647 - HP15_2085 0.72 +0.4 2,183,673 + HP15_2085 0.78 +0.4 2,183,681 - HP15_2085 0.80 +0.5
Or see this region's nucleotide sequence