Experiment: monoculture; Experiment B, time point 5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_720 and HP15_721 are separated by 14 nucleotides HP15_721 and HP15_722 are separated by 224 nucleotides
HP15_720: HP15_720 - succinylglutamate desuccinylase/aspartoacylase family protein, at 728,614 to 729,678
_720
HP15_721: HP15_721 - protein belonging to uncharacterized protein family UPF0047, at 729,693 to 730,118
_721
HP15_722: HP15_722 - TRAP transporter for fumarate, succinate, L-malate, and 2-oxoglutarate, solute receptor component (from data) , at 730,343 to 731,320
_722
Position (kb)
729
730
731 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 728.694 kb on + strand at 728.710 kb on + strand at 728.718 kb on - strand at 728.793 kb on + strand, within HP15_720 at 728.826 kb on + strand, within HP15_720 at 728.906 kb on + strand, within HP15_720 at 728.914 kb on - strand, within HP15_720 at 728.914 kb on - strand, within HP15_720 at 728.932 kb on - strand, within HP15_720 at 728.937 kb on - strand, within HP15_720 at 729.120 kb on - strand, within HP15_720 at 729.223 kb on + strand, within HP15_720 at 729.269 kb on - strand, within HP15_720 at 729.309 kb on + strand, within HP15_720 at 729.315 kb on + strand, within HP15_720 at 729.427 kb on + strand, within HP15_720 at 729.428 kb on + strand, within HP15_720 at 729.435 kb on - strand, within HP15_720 at 729.481 kb on + strand, within HP15_720 at 729.494 kb on - strand, within HP15_720 at 729.654 kb on + strand at 729.654 kb on + strand at 729.654 kb on + strand at 729.654 kb on + strand at 729.664 kb on - strand at 729.698 kb on - strand at 729.731 kb on + strand at 729.739 kb on + strand, within HP15_721 at 729.783 kb on - strand, within HP15_721 at 729.914 kb on - strand, within HP15_721 at 730.305 kb on - strand at 730.417 kb on - strand at 730.417 kb on - strand at 730.417 kb on - strand at 730.556 kb on + strand, within HP15_722 at 730.564 kb on - strand, within HP15_722 at 730.564 kb on - strand, within HP15_722 at 730.683 kb on - strand, within HP15_722 at 730.739 kb on - strand, within HP15_722 at 730.828 kb on + strand, within HP15_722 at 730.869 kb on + strand, within HP15_722 at 730.877 kb on - strand, within HP15_722 at 730.906 kb on - strand, within HP15_722 at 730.941 kb on - strand, within HP15_722 at 730.962 kb on - strand, within HP15_722 at 731.022 kb on + strand, within HP15_722 at 731.113 kb on + strand, within HP15_722
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 5 remove 728,694 + -0.4 728,710 + -0.6 728,718 - -0.6 728,793 + HP15_720 0.17 +1.4 728,826 + HP15_720 0.20 -0.1 728,906 + HP15_720 0.27 -1.0 728,914 - HP15_720 0.28 -1.0 728,914 - HP15_720 0.28 -0.1 728,932 - HP15_720 0.30 +2.4 728,937 - HP15_720 0.30 +1.8 729,120 - HP15_720 0.48 +2.1 729,223 + HP15_720 0.57 -0.4 729,269 - HP15_720 0.62 +0.0 729,309 + HP15_720 0.65 +0.4 729,315 + HP15_720 0.66 -0.7 729,427 + HP15_720 0.76 -0.3 729,428 + HP15_720 0.76 +1.3 729,435 - HP15_720 0.77 +3.3 729,481 + HP15_720 0.81 -0.5 729,494 - HP15_720 0.83 -0.5 729,654 + +0.8 729,654 + +1.7 729,654 + -1.1 729,654 + -0.4 729,664 - -0.3 729,698 - +1.6 729,731 + +1.0 729,739 + HP15_721 0.11 +0.5 729,783 - HP15_721 0.21 +0.1 729,914 - HP15_721 0.52 -1.2 730,305 - -0.0 730,417 - -0.3 730,417 - -0.3 730,417 - +0.2 730,556 + HP15_722 0.22 +1.9 730,564 - HP15_722 0.23 -1.0 730,564 - HP15_722 0.23 -4.1 730,683 - HP15_722 0.35 +2.0 730,739 - HP15_722 0.40 -0.7 730,828 + HP15_722 0.50 -1.7 730,869 + HP15_722 0.54 +0.6 730,877 - HP15_722 0.55 -0.2 730,906 - HP15_722 0.58 +1.0 730,941 - HP15_722 0.61 -0.1 730,962 - HP15_722 0.63 -1.1 731,022 + HP15_722 0.69 -0.0 731,113 + HP15_722 0.79 +1.9
Or see this region's nucleotide sequence