Experiment: monoculture; Experiment B, time point 5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_324 and HP15_325 are separated by 59 nucleotides HP15_325 and HP15_326 overlap by 4 nucleotides HP15_326 and HP15_327 are separated by 28 nucleotides HP15_327 and HP15_328 are separated by 469 nucleotides
HP15_324: HP15_324 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 320,847 to 321,641
_324
HP15_325: HP15_325 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase, at 321,701 to 322,198
_325
HP15_326: HP15_326 - polyA polymerase, at 322,195 to 323,526
_326
HP15_327: HP15_327 - hypothetical protein, at 323,555 to 323,764
_327
HP15_328: HP15_328 - two component, sigma54 specific, transcriptional regulator, Fis family, at 324,234 to 325,637
_328
Position (kb) 322
323
324 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 321.670 kb on - strand at 322.261 kb on - strand at 322.273 kb on - strand at 322.293 kb on - strand at 322.382 kb on + strand, within HP15_326 at 322.562 kb on - strand, within HP15_326 at 322.573 kb on - strand, within HP15_326 at 322.577 kb on - strand, within HP15_326 at 322.604 kb on + strand, within HP15_326 at 322.746 kb on - strand, within HP15_326 at 322.748 kb on - strand, within HP15_326 at 322.753 kb on - strand, within HP15_326 at 322.789 kb on + strand, within HP15_326 at 322.789 kb on + strand, within HP15_326 at 322.816 kb on - strand, within HP15_326 at 322.955 kb on + strand, within HP15_326 at 323.110 kb on + strand, within HP15_326 at 323.118 kb on - strand, within HP15_326 at 323.200 kb on - strand, within HP15_326 at 323.503 kb on - strand at 323.659 kb on - strand, within HP15_327 at 323.789 kb on - strand at 324.122 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 5 remove 321,670 - +0.3 322,261 - +0.7 322,273 - +0.1 322,293 - +3.0 322,382 + HP15_326 0.14 -3.0 322,562 - HP15_326 0.28 -1.4 322,573 - HP15_326 0.28 -1.7 322,577 - HP15_326 0.29 +0.1 322,604 + HP15_326 0.31 -2.2 322,746 - HP15_326 0.41 +0.1 322,748 - HP15_326 0.42 +0.3 322,753 - HP15_326 0.42 +1.6 322,789 + HP15_326 0.45 -3.0 322,789 + HP15_326 0.45 -2.7 322,816 - HP15_326 0.47 -1.3 322,955 + HP15_326 0.57 -1.5 323,110 + HP15_326 0.69 -1.5 323,118 - HP15_326 0.69 -2.2 323,200 - HP15_326 0.75 -1.9 323,503 - -0.4 323,659 - HP15_327 0.50 -0.6 323,789 - +3.5 324,122 + +2.6
Or see this region's nucleotide sequence