Experiment: monoculture; Experiment B, time point 5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_284 and HP15_285 are separated by 127 nucleotides HP15_285 and HP15_286 are separated by 243 nucleotides HP15_286 and HP15_287 are separated by 22 nucleotides
HP15_284: HP15_284 - homoserine O-acetyltransferase, at 274,136 to 275,284
_284
HP15_285: HP15_285 - conserved hypothetical protein, at 275,412 to 277,376
_285
HP15_286: HP15_286 - protein of unknown function YGGT, at 277,620 to 278,204
_286
HP15_287: HP15_287 - pyrroline-5-carboxylate reductase, at 278,227 to 279,060
_287
Position (kb)
275
276
277
278 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 274.425 kb on - strand, within HP15_284 at 274.573 kb on - strand, within HP15_284 at 274.612 kb on + strand, within HP15_284 at 274.612 kb on + strand, within HP15_284 at 274.620 kb on - strand, within HP15_284 at 274.786 kb on - strand, within HP15_284 at 274.983 kb on - strand, within HP15_284 at 275.026 kb on + strand, within HP15_284 at 275.074 kb on - strand, within HP15_284 at 275.365 kb on - strand at 275.365 kb on - strand at 275.365 kb on - strand at 275.418 kb on - strand at 275.624 kb on + strand, within HP15_285 at 275.632 kb on - strand, within HP15_285 at 275.699 kb on - strand, within HP15_285 at 275.699 kb on - strand, within HP15_285 at 275.851 kb on + strand, within HP15_285 at 275.860 kb on - strand, within HP15_285 at 275.993 kb on + strand, within HP15_285 at 276.088 kb on + strand, within HP15_285 at 276.132 kb on - strand, within HP15_285 at 276.147 kb on + strand, within HP15_285 at 276.180 kb on + strand, within HP15_285 at 276.195 kb on + strand, within HP15_285 at 276.198 kb on - strand, within HP15_285 at 276.198 kb on - strand, within HP15_285 at 276.203 kb on - strand, within HP15_285 at 276.226 kb on - strand, within HP15_285 at 276.246 kb on + strand, within HP15_285 at 276.292 kb on + strand, within HP15_285 at 276.310 kb on - strand, within HP15_285 at 276.335 kb on - strand, within HP15_285 at 276.435 kb on + strand, within HP15_285 at 276.453 kb on - strand, within HP15_285 at 276.530 kb on + strand, within HP15_285 at 276.531 kb on - strand, within HP15_285 at 276.535 kb on + strand, within HP15_285 at 276.621 kb on - strand, within HP15_285 at 276.666 kb on + strand, within HP15_285 at 276.666 kb on + strand, within HP15_285 at 276.674 kb on - strand, within HP15_285 at 276.679 kb on + strand, within HP15_285 at 276.786 kb on + strand, within HP15_285 at 276.857 kb on + strand, within HP15_285 at 276.889 kb on + strand, within HP15_285 at 276.895 kb on + strand, within HP15_285 at 276.964 kb on - strand, within HP15_285 at 277.135 kb on - strand, within HP15_285 at 277.138 kb on + strand, within HP15_285 at 277.138 kb on + strand, within HP15_285 at 277.138 kb on + strand, within HP15_285 at 277.146 kb on - strand, within HP15_285 at 277.146 kb on - strand, within HP15_285 at 277.158 kb on - strand, within HP15_285 at 277.231 kb on - strand at 277.256 kb on + strand at 277.339 kb on + strand at 277.360 kb on + strand at 277.598 kb on - strand at 277.641 kb on + strand at 277.641 kb on + strand at 277.721 kb on + strand, within HP15_286 at 277.721 kb on + strand, within HP15_286 at 277.729 kb on - strand, within HP15_286 at 277.729 kb on - strand, within HP15_286 at 277.729 kb on - strand, within HP15_286 at 277.729 kb on - strand, within HP15_286 at 277.729 kb on - strand, within HP15_286 at 277.749 kb on + strand, within HP15_286 at 277.749 kb on - strand, within HP15_286 at 277.884 kb on - strand, within HP15_286 at 277.890 kb on + strand, within HP15_286 at 277.895 kb on + strand, within HP15_286 at 277.896 kb on - strand, within HP15_286 at 277.905 kb on + strand, within HP15_286 at 277.971 kb on + strand, within HP15_286 at 277.972 kb on - strand, within HP15_286 at 277.977 kb on + strand, within HP15_286 at 278.035 kb on - strand, within HP15_286 at 278.153 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 5 remove 274,425 - HP15_284 0.25 -3.6 274,573 - HP15_284 0.38 -1.4 274,612 + HP15_284 0.41 -1.5 274,612 + HP15_284 0.41 -4.5 274,620 - HP15_284 0.42 -2.0 274,786 - HP15_284 0.57 -4.0 274,983 - HP15_284 0.74 -1.3 275,026 + HP15_284 0.77 -3.8 275,074 - HP15_284 0.82 -2.2 275,365 - -0.5 275,365 - -1.2 275,365 - -2.5 275,418 - -1.3 275,624 + HP15_285 0.11 +0.3 275,632 - HP15_285 0.11 -0.7 275,699 - HP15_285 0.15 +0.0 275,699 - HP15_285 0.15 -0.9 275,851 + HP15_285 0.22 -0.3 275,860 - HP15_285 0.23 -0.7 275,993 + HP15_285 0.30 -0.5 276,088 + HP15_285 0.34 -0.3 276,132 - HP15_285 0.37 -1.1 276,147 + HP15_285 0.37 +0.1 276,180 + HP15_285 0.39 +2.2 276,195 + HP15_285 0.40 -0.0 276,198 - HP15_285 0.40 +2.0 276,198 - HP15_285 0.40 +2.1 276,203 - HP15_285 0.40 -0.5 276,226 - HP15_285 0.41 -1.6 276,246 + HP15_285 0.42 +2.5 276,292 + HP15_285 0.45 +1.6 276,310 - HP15_285 0.46 +0.4 276,335 - HP15_285 0.47 -0.2 276,435 + HP15_285 0.52 +1.6 276,453 - HP15_285 0.53 -0.4 276,530 + HP15_285 0.57 +0.8 276,531 - HP15_285 0.57 -1.0 276,535 + HP15_285 0.57 +2.6 276,621 - HP15_285 0.62 -1.3 276,666 + HP15_285 0.64 +1.8 276,666 + HP15_285 0.64 +2.4 276,674 - HP15_285 0.64 +2.7 276,679 + HP15_285 0.64 +0.6 276,786 + HP15_285 0.70 -0.3 276,857 + HP15_285 0.74 +3.2 276,889 + HP15_285 0.75 +1.9 276,895 + HP15_285 0.75 +1.8 276,964 - HP15_285 0.79 -1.3 277,135 - HP15_285 0.88 +0.6 277,138 + HP15_285 0.88 +0.0 277,138 + HP15_285 0.88 -0.0 277,138 + HP15_285 0.88 -0.7 277,146 - HP15_285 0.88 +2.1 277,146 - HP15_285 0.88 -2.5 277,158 - HP15_285 0.89 -0.3 277,231 - +1.8 277,256 + +2.3 277,339 + -0.8 277,360 + +0.5 277,598 - -0.2 277,641 + -0.9 277,641 + -2.6 277,721 + HP15_286 0.17 -1.3 277,721 + HP15_286 0.17 +0.7 277,729 - HP15_286 0.19 -1.4 277,729 - HP15_286 0.19 -0.6 277,729 - HP15_286 0.19 +2.2 277,729 - HP15_286 0.19 +2.2 277,729 - HP15_286 0.19 +2.0 277,749 + HP15_286 0.22 -0.4 277,749 - HP15_286 0.22 +1.1 277,884 - HP15_286 0.45 +2.2 277,890 + HP15_286 0.46 -3.0 277,895 + HP15_286 0.47 +0.1 277,896 - HP15_286 0.47 +2.1 277,905 + HP15_286 0.49 +0.7 277,971 + HP15_286 0.60 +0.7 277,972 - HP15_286 0.60 -1.4 277,977 + HP15_286 0.61 -1.5 278,035 - HP15_286 0.71 +3.1 278,153 + +0.0
Or see this region's nucleotide sequence