Experiment: monoculture; Experiment B, time point 5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_219 and HP15_220 are separated by 150 nucleotides HP15_220 and HP15_222 overlap by 18 nucleotides HP15_222 and HP15_221 are separated by 42 nucleotides HP15_221 and HP15_223 are separated by 95 nucleotides HP15_223 and HP15_224 are separated by 124 nucleotides
HP15_219: HP15_219 - flagellar basal body-associated protein FliL, at 207,622 to 208,071
_219
HP15_220: HP15_220 - disulfide bond formation protein B, at 208,222 to 208,629
_220
HP15_222: HP15_222 - hypothetical protein, at 208,612 to 208,728
_222
HP15_221: HP15_221 - anti-RNA polymerase sigma 70 factor, at 208,771 to 209,250
_221
HP15_223: HP15_223 - peptidylprolyl isomerase, FKBP-type, at 209,346 to 210,062
_223
HP15_224: HP15_224 - conserved hypothetical protein, secreted, at 210,187 to 210,573
_224
Position (kb) 208
209
210 Strain fitness (log2 ratio) -1
0
1
2 at 208.110 kb on + strand at 208.282 kb on - strand, within HP15_220 at 208.308 kb on + strand, within HP15_220 at 208.444 kb on - strand, within HP15_220 at 208.486 kb on - strand, within HP15_220 at 208.505 kb on - strand, within HP15_220 at 208.564 kb on + strand, within HP15_220 at 208.572 kb on - strand, within HP15_220 at 208.811 kb on + strand at 208.835 kb on + strand, within HP15_221 at 208.868 kb on + strand, within HP15_221 at 209.032 kb on - strand, within HP15_221 at 209.368 kb on + strand at 209.397 kb on + strand at 209.402 kb on + strand at 209.405 kb on - strand at 209.543 kb on - strand, within HP15_223 at 209.789 kb on + strand, within HP15_223 at 210.005 kb on + strand at 210.090 kb on + strand at 210.098 kb on - strand at 210.107 kb on + strand at 210.150 kb on - strand at 210.238 kb on + strand, within HP15_224
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 5 remove 208,110 + -0.6 208,282 - HP15_220 0.15 -1.3 208,308 + HP15_220 0.21 -1.4 208,444 - HP15_220 0.54 +0.1 208,486 - HP15_220 0.65 -1.0 208,505 - HP15_220 0.69 -0.0 208,564 + HP15_220 0.84 -1.5 208,572 - HP15_220 0.86 +0.4 208,811 + -0.7 208,835 + HP15_221 0.13 +1.8 208,868 + HP15_221 0.20 +1.9 209,032 - HP15_221 0.54 +2.4 209,368 + -1.5 209,397 + +0.1 209,402 + -0.5 209,405 - +2.2 209,543 - HP15_223 0.27 +1.6 209,789 + HP15_223 0.62 +0.2 210,005 + -0.3 210,090 + +0.3 210,098 - -0.2 210,107 + -1.6 210,150 - -0.4 210,238 + HP15_224 0.13 -0.5
Or see this region's nucleotide sequence