Experiment: monoculture; Experiment B, time point 5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1366 and HP15_1367 are separated by 134 nucleotides HP15_1367 and HP15_1368 are separated by 59 nucleotides HP15_1368 and HP15_1369 overlap by 1 nucleotides HP15_1369 and HP15_1370 are separated by 26 nucleotides
HP15_1366: HP15_1366 - ribosomal S1 synthesis/modification protein, at 1,438,618 to 1,440,312
_1366
HP15_1367: HP15_1367 - integration host factor, beta subunit, at 1,440,447 to 1,440,743
_1367
HP15_1368: HP15_1368 - membrane protein containing DUF1049, at 1,440,803 to 1,441,105
_1368
HP15_1369: HP15_1369 - conserved hypothetical protein, at 1,441,105 to 1,442,274
_1369
HP15_1370: HP15_1370 - orotidine 5'-phosphate decarboxylase, at 1,442,301 to 1,443,017
_1370
Position (kb)
1441
1442
1443 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1440.776 kb on + strand at 1441.256 kb on + strand, within HP15_1369 at 1441.519 kb on + strand, within HP15_1369 at 1441.535 kb on + strand, within HP15_1369 at 1441.554 kb on + strand, within HP15_1369 at 1441.647 kb on + strand, within HP15_1369 at 1441.796 kb on + strand, within HP15_1369 at 1441.883 kb on - strand, within HP15_1369 at 1442.064 kb on + strand, within HP15_1369 at 1442.064 kb on + strand, within HP15_1369 at 1442.097 kb on + strand, within HP15_1369 at 1442.269 kb on + strand at 1442.269 kb on + strand at 1443.137 kb on + strand at 1443.139 kb on + strand at 1443.177 kb on - strand at 1443.182 kb on - strand at 1443.229 kb on + strand at 1443.237 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 5 remove 1,440,776 + +1.1 1,441,256 + HP15_1369 0.13 -1.7 1,441,519 + HP15_1369 0.35 +0.8 1,441,535 + HP15_1369 0.37 -1.3 1,441,554 + HP15_1369 0.38 -0.5 1,441,647 + HP15_1369 0.46 -1.6 1,441,796 + HP15_1369 0.59 -2.4 1,441,883 - HP15_1369 0.66 -5.4 1,442,064 + HP15_1369 0.82 +1.2 1,442,064 + HP15_1369 0.82 -0.9 1,442,097 + HP15_1369 0.85 -1.1 1,442,269 + -1.6 1,442,269 + -1.0 1,443,137 + -1.0 1,443,139 + -1.6 1,443,177 - +0.4 1,443,182 - +0.2 1,443,229 + -1.2 1,443,237 - -0.9
Or see this region's nucleotide sequence