Experiment: monoculture; Experiment B, time point 5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1000 and HP15_1001 are separated by 14 nucleotides HP15_1001 and HP15_1002 overlap by 1 nucleotides HP15_1002 and HP15_1003 are separated by 2 nucleotides HP15_1003 and HP15_1004 are separated by 14 nucleotides
HP15_1000: HP15_1000 - uncharacterized peroxidase-related protein, at 1,009,927 to 1,010,502
_1000
HP15_1001: HP15_1001 - pyruvate carboxyltransferase, at 1,010,517 to 1,011,422
_1001
HP15_1002: HP15_1002 - methylcrotonyl-CoA carboxylase, alpha subunit, at 1,011,422 to 1,013,416
_1002
HP15_1003: HP15_1003 - enoyl-CoA hydratase/isomerase, at 1,013,419 to 1,014,225
_1003
HP15_1004: HP15_1004 - 3-methylcrotonyl CoA carboxylase, beta subunit, at 1,014,240 to 1,015,847
_1004
Position (kb)
1011
1012
1013
1014 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1014.142 kb on + strand, within HP15_1003 at 1014.150 kb on - strand at 1014.166 kb on + strand at 1014.166 kb on + strand at 1014.299 kb on + strand at 1014.303 kb on - strand at 1014.307 kb on - strand at 1014.307 kb on - strand at 1014.321 kb on + strand at 1014.324 kb on - strand at 1014.329 kb on - strand at 1014.329 kb on - strand at 1014.334 kb on - strand at 1014.370 kb on + strand at 1014.374 kb on - strand at 1014.381 kb on + strand at 1014.381 kb on + strand at 1014.389 kb on + strand at 1014.389 kb on + strand at 1014.389 kb on - strand at 1014.389 kb on - strand at 1014.389 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 5 remove 1,014,142 + HP15_1003 0.90 +0.4 1,014,150 - +0.2 1,014,166 + -0.3 1,014,166 + -1.0 1,014,299 + -1.1 1,014,303 - +1.2 1,014,307 - +0.2 1,014,307 - +1.8 1,014,321 + +0.5 1,014,324 - +1.2 1,014,329 - +1.3 1,014,329 - -1.0 1,014,334 - +0.0 1,014,370 + -1.0 1,014,374 - +0.4 1,014,381 + +0.8 1,014,381 + -0.0 1,014,389 + -1.5 1,014,389 + -0.8 1,014,389 - -2.4 1,014,389 - -2.3 1,014,389 - +0.0
Or see this region's nucleotide sequence