Experiment: monoculture; Experiment B, time point 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_12 and HP15_13 are separated by 143 nucleotides HP15_13 and HP15_14 are separated by 38 nucleotides HP15_14 and HP15_15 are separated by 319 nucleotides HP15_15 and HP15_16 are separated by 7 nucleotides
HP15_12: HP15_12 - enoyl-CoA hydratase/isomerase, at 14,943 to 15,755
_12
HP15_13: HP15_13 - short-chain dehydrogenase/reductase SDR, at 15,899 to 16,627
_13
HP15_14: HP15_14 - transcriptional regulator, TetR family, at 16,666 to 17,247
_14
HP15_15: HP15_15 - short chain dehydrogenase/reductase, at 17,567 to 18,214
_15
HP15_16: HP15_16 - glyoxalase/bleomycin resistance protein/dioxygenase-like protein, at 18,222 to 18,392
_16
Position (kb)
16
17
18 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 15.839 kb on + strand at 15.851 kb on + strand at 16.008 kb on + strand, within HP15_13 at 16.015 kb on + strand, within HP15_13 at 16.069 kb on - strand, within HP15_13 at 16.079 kb on - strand, within HP15_13 at 16.086 kb on + strand, within HP15_13 at 16.100 kb on - strand, within HP15_13 at 16.162 kb on + strand, within HP15_13 at 16.162 kb on + strand, within HP15_13 at 16.162 kb on + strand, within HP15_13 at 16.162 kb on + strand, within HP15_13 at 16.170 kb on - strand, within HP15_13 at 16.182 kb on + strand, within HP15_13 at 16.183 kb on - strand, within HP15_13 at 16.183 kb on - strand, within HP15_13 at 16.207 kb on + strand, within HP15_13 at 16.242 kb on + strand, within HP15_13 at 16.247 kb on + strand, within HP15_13 at 16.250 kb on - strand, within HP15_13 at 16.250 kb on - strand, within HP15_13 at 16.280 kb on + strand, within HP15_13 at 16.291 kb on + strand, within HP15_13 at 16.291 kb on + strand, within HP15_13 at 16.291 kb on + strand, within HP15_13 at 16.299 kb on - strand, within HP15_13 at 16.404 kb on + strand, within HP15_13 at 16.412 kb on - strand, within HP15_13 at 16.413 kb on + strand, within HP15_13 at 16.413 kb on + strand, within HP15_13 at 16.421 kb on - strand, within HP15_13 at 16.421 kb on - strand, within HP15_13 at 16.421 kb on - strand, within HP15_13 at 16.460 kb on - strand, within HP15_13 at 16.504 kb on - strand, within HP15_13 at 16.563 kb on + strand at 16.567 kb on + strand at 16.571 kb on - strand at 16.571 kb on - strand at 16.606 kb on + strand at 16.614 kb on - strand at 16.614 kb on - strand at 16.619 kb on + strand at 16.620 kb on + strand at 16.627 kb on - strand at 16.629 kb on - strand at 16.714 kb on - strand at 16.729 kb on - strand, within HP15_14 at 16.729 kb on - strand, within HP15_14 at 16.731 kb on + strand, within HP15_14 at 16.731 kb on + strand, within HP15_14 at 16.734 kb on - strand, within HP15_14 at 16.795 kb on + strand, within HP15_14 at 16.795 kb on + strand, within HP15_14 at 17.048 kb on - strand, within HP15_14 at 17.070 kb on + strand, within HP15_14 at 17.089 kb on - strand, within HP15_14 at 17.092 kb on + strand, within HP15_14 at 17.100 kb on - strand, within HP15_14 at 17.102 kb on + strand, within HP15_14 at 17.227 kb on + strand at 17.227 kb on + strand at 17.391 kb on - strand at 17.455 kb on + strand at 17.460 kb on + strand at 17.486 kb on + strand at 17.486 kb on + strand at 17.494 kb on - strand at 17.494 kb on - strand at 17.505 kb on + strand at 17.519 kb on + strand at 17.627 kb on + strand at 17.627 kb on + strand at 17.629 kb on + strand at 17.635 kb on - strand, within HP15_15 at 17.635 kb on - strand, within HP15_15 at 17.637 kb on - strand, within HP15_15 at 17.664 kb on + strand, within HP15_15 at 17.667 kb on - strand, within HP15_15 at 17.670 kb on + strand, within HP15_15 at 17.673 kb on - strand, within HP15_15 at 17.673 kb on - strand, within HP15_15 at 17.676 kb on - strand, within HP15_15 at 17.706 kb on + strand, within HP15_15 at 17.706 kb on + strand, within HP15_15 at 17.707 kb on + strand, within HP15_15 at 17.745 kb on + strand, within HP15_15 at 17.756 kb on - strand, within HP15_15 at 17.770 kb on + strand, within HP15_15 at 17.791 kb on + strand, within HP15_15 at 17.803 kb on + strand, within HP15_15 at 17.811 kb on - strand, within HP15_15 at 17.929 kb on + strand, within HP15_15 at 18.008 kb on + strand, within HP15_15 at 18.008 kb on + strand, within HP15_15 at 18.018 kb on - strand, within HP15_15 at 18.028 kb on - strand, within HP15_15 at 18.107 kb on + strand, within HP15_15 at 18.115 kb on - strand, within HP15_15 at 18.155 kb on + strand at 18.211 kb on - strand at 18.217 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 4 remove 15,839 + +1.0 15,851 + +0.2 16,008 + HP15_13 0.15 -0.9 16,015 + HP15_13 0.16 +0.6 16,069 - HP15_13 0.23 -0.5 16,079 - HP15_13 0.25 +0.8 16,086 + HP15_13 0.26 -0.3 16,100 - HP15_13 0.28 +1.0 16,162 + HP15_13 0.36 -1.8 16,162 + HP15_13 0.36 +0.9 16,162 + HP15_13 0.36 -2.2 16,162 + HP15_13 0.36 +0.9 16,170 - HP15_13 0.37 -0.8 16,182 + HP15_13 0.39 +1.2 16,183 - HP15_13 0.39 -0.0 16,183 - HP15_13 0.39 +0.4 16,207 + HP15_13 0.42 -0.5 16,242 + HP15_13 0.47 -0.9 16,247 + HP15_13 0.48 -0.1 16,250 - HP15_13 0.48 -0.3 16,250 - HP15_13 0.48 +0.8 16,280 + HP15_13 0.52 -2.4 16,291 + HP15_13 0.54 -1.3 16,291 + HP15_13 0.54 -0.6 16,291 + HP15_13 0.54 -1.2 16,299 - HP15_13 0.55 +1.4 16,404 + HP15_13 0.69 -1.4 16,412 - HP15_13 0.70 +1.7 16,413 + HP15_13 0.71 -2.9 16,413 + HP15_13 0.71 +2.0 16,421 - HP15_13 0.72 +1.0 16,421 - HP15_13 0.72 +0.0 16,421 - HP15_13 0.72 +1.0 16,460 - HP15_13 0.77 +1.2 16,504 - HP15_13 0.83 +1.0 16,563 + +1.0 16,567 + +0.1 16,571 - -0.4 16,571 - -1.6 16,606 + +0.7 16,614 - +1.6 16,614 - +0.4 16,619 + -0.6 16,620 + +0.0 16,627 - -0.1 16,629 - +0.7 16,714 - +0.5 16,729 - HP15_14 0.11 -1.6 16,729 - HP15_14 0.11 -1.2 16,731 + HP15_14 0.11 -0.9 16,731 + HP15_14 0.11 +0.8 16,734 - HP15_14 0.12 -0.7 16,795 + HP15_14 0.22 -0.3 16,795 + HP15_14 0.22 +1.1 17,048 - HP15_14 0.66 -0.5 17,070 + HP15_14 0.69 +0.0 17,089 - HP15_14 0.73 -0.7 17,092 + HP15_14 0.73 +1.8 17,100 - HP15_14 0.75 -0.2 17,102 + HP15_14 0.75 -0.4 17,227 + -1.1 17,227 + +0.1 17,391 - -0.1 17,455 + -1.0 17,460 + -1.1 17,486 + -0.4 17,486 + +1.3 17,494 - +1.2 17,494 - +0.4 17,505 + -0.9 17,519 + +1.3 17,627 + +0.2 17,627 + +2.1 17,629 + +1.0 17,635 - HP15_15 0.10 -0.2 17,635 - HP15_15 0.10 -0.9 17,637 - HP15_15 0.11 +1.4 17,664 + HP15_15 0.15 -1.2 17,667 - HP15_15 0.15 -0.5 17,670 + HP15_15 0.16 +0.4 17,673 - HP15_15 0.16 -0.9 17,673 - HP15_15 0.16 +1.1 17,676 - HP15_15 0.17 +1.3 17,706 + HP15_15 0.21 -1.4 17,706 + HP15_15 0.21 +0.1 17,707 + HP15_15 0.22 -1.3 17,745 + HP15_15 0.27 -1.0 17,756 - HP15_15 0.29 +0.8 17,770 + HP15_15 0.31 -0.5 17,791 + HP15_15 0.35 -0.3 17,803 + HP15_15 0.36 +0.8 17,811 - HP15_15 0.38 +1.0 17,929 + HP15_15 0.56 +1.0 18,008 + HP15_15 0.68 +0.0 18,008 + HP15_15 0.68 -0.3 18,018 - HP15_15 0.70 +1.8 18,028 - HP15_15 0.71 -1.8 18,107 + HP15_15 0.83 +1.2 18,115 - HP15_15 0.85 -0.7 18,155 + -1.6 18,211 - -0.8 18,217 + -0.2
Or see this region's nucleotide sequence