Experiment: monoculture; Experiment B, time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_54 and HP15_55 are separated by 10 nucleotides HP15_55 and HP15_56 are separated by 24 nucleotides HP15_56 and HP15_57 are separated by 32 nucleotides HP15_57 and HP15_58 are separated by 45 nucleotides
HP15_54: HP15_54 - acyl-CoA dehydrogenase domain protein, at 59,443 to 60,501
_54
HP15_55: HP15_55 - enoyl-CoA hydratase/isomerase, at 60,512 to 61,312
_55
HP15_56: HP15_56 - acyl-CoA dehydrogenase domain protein, at 61,337 to 62,461
_56
HP15_57: HP15_57 - conserved hypothetical protein, at 62,494 to 63,393
_57
HP15_58: HP15_58 - GTP cyclohydrolase 1, at 63,439 to 64,068
_58
Position (kb)
61
62
63 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 60.337 kb on + strand, within HP15_54 at 60.338 kb on - strand, within HP15_54 at 60.338 kb on - strand, within HP15_54 at 60.369 kb on + strand, within HP15_54 at 60.397 kb on - strand at 60.439 kb on - strand at 60.570 kb on + strand at 60.577 kb on + strand at 60.578 kb on - strand at 60.578 kb on - strand at 60.635 kb on + strand, within HP15_55 at 60.637 kb on + strand, within HP15_55 at 60.637 kb on + strand, within HP15_55 at 60.637 kb on + strand, within HP15_55 at 60.643 kb on - strand, within HP15_55 at 60.645 kb on - strand, within HP15_55 at 60.645 kb on - strand, within HP15_55 at 60.701 kb on + strand, within HP15_55 at 60.709 kb on + strand, within HP15_55 at 60.709 kb on + strand, within HP15_55 at 60.741 kb on - strand, within HP15_55 at 61.046 kb on + strand, within HP15_55 at 61.046 kb on + strand, within HP15_55 at 61.103 kb on + strand, within HP15_55 at 61.103 kb on + strand, within HP15_55 at 61.103 kb on + strand, within HP15_55 at 61.110 kb on + strand, within HP15_55 at 61.111 kb on - strand, within HP15_55 at 61.118 kb on - strand, within HP15_55 at 61.118 kb on - strand, within HP15_55 at 61.128 kb on - strand, within HP15_55 at 61.168 kb on + strand, within HP15_55 at 61.186 kb on - strand, within HP15_55 at 61.191 kb on - strand, within HP15_55 at 61.212 kb on - strand, within HP15_55 at 61.221 kb on - strand, within HP15_55 at 61.221 kb on - strand, within HP15_55 at 61.489 kb on + strand, within HP15_56 at 61.499 kb on - strand, within HP15_56 at 61.502 kb on - strand, within HP15_56 at 61.550 kb on - strand, within HP15_56 at 61.561 kb on + strand, within HP15_56 at 61.733 kb on - strand, within HP15_56 at 61.751 kb on + strand, within HP15_56 at 61.759 kb on - strand, within HP15_56 at 61.852 kb on - strand, within HP15_56 at 61.943 kb on + strand, within HP15_56 at 61.943 kb on + strand, within HP15_56 at 61.951 kb on - strand, within HP15_56 at 61.956 kb on - strand, within HP15_56 at 61.956 kb on - strand, within HP15_56 at 61.988 kb on + strand, within HP15_56 at 61.988 kb on + strand, within HP15_56 at 61.998 kb on + strand, within HP15_56 at 62.026 kb on - strand, within HP15_56 at 62.218 kb on - strand, within HP15_56 at 62.243 kb on + strand, within HP15_56 at 62.250 kb on + strand, within HP15_56 at 62.266 kb on + strand, within HP15_56 at 62.287 kb on + strand, within HP15_56 at 62.303 kb on - strand, within HP15_56 at 62.346 kb on - strand, within HP15_56 at 62.415 kb on - strand at 62.481 kb on + strand at 62.487 kb on - strand at 62.516 kb on - strand at 62.536 kb on - strand at 62.577 kb on - strand at 62.590 kb on - strand, within HP15_57 at 62.649 kb on - strand, within HP15_57 at 62.654 kb on - strand, within HP15_57 at 62.656 kb on + strand, within HP15_57 at 62.666 kb on + strand, within HP15_57 at 62.682 kb on + strand, within HP15_57 at 62.786 kb on + strand, within HP15_57 at 62.799 kb on - strand, within HP15_57 at 62.837 kb on + strand, within HP15_57 at 62.837 kb on + strand, within HP15_57 at 62.873 kb on - strand, within HP15_57 at 62.888 kb on - strand, within HP15_57 at 62.920 kb on - strand, within HP15_57 at 63.140 kb on + strand, within HP15_57 at 63.201 kb on + strand, within HP15_57 at 63.313 kb on - strand at 63.343 kb on - strand at 63.461 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 3 remove 60,337 + HP15_54 0.84 -1.5 60,338 - HP15_54 0.85 +1.0 60,338 - HP15_54 0.85 +0.3 60,369 + HP15_54 0.87 +0.8 60,397 - +1.2 60,439 - -1.0 60,570 + +0.6 60,577 + +0.2 60,578 - -0.4 60,578 - -2.3 60,635 + HP15_55 0.15 +0.5 60,637 + HP15_55 0.16 -0.1 60,637 + HP15_55 0.16 -0.2 60,637 + HP15_55 0.16 +0.2 60,643 - HP15_55 0.16 -0.5 60,645 - HP15_55 0.17 -0.1 60,645 - HP15_55 0.17 +0.3 60,701 + HP15_55 0.24 +0.5 60,709 + HP15_55 0.25 +0.9 60,709 + HP15_55 0.25 +0.1 60,741 - HP15_55 0.29 -1.1 61,046 + HP15_55 0.67 +0.4 61,046 + HP15_55 0.67 +0.8 61,103 + HP15_55 0.74 +0.2 61,103 + HP15_55 0.74 +1.7 61,103 + HP15_55 0.74 -0.6 61,110 + HP15_55 0.75 +0.6 61,111 - HP15_55 0.75 -2.0 61,118 - HP15_55 0.76 -0.5 61,118 - HP15_55 0.76 +2.0 61,128 - HP15_55 0.77 +0.9 61,168 + HP15_55 0.82 +2.1 61,186 - HP15_55 0.84 -0.1 61,191 - HP15_55 0.85 -0.1 61,212 - HP15_55 0.87 +0.6 61,221 - HP15_55 0.89 +0.1 61,221 - HP15_55 0.89 +0.7 61,489 + HP15_56 0.14 -0.3 61,499 - HP15_56 0.14 -1.0 61,502 - HP15_56 0.15 +0.7 61,550 - HP15_56 0.19 -0.9 61,561 + HP15_56 0.20 -0.7 61,733 - HP15_56 0.35 -1.0 61,751 + HP15_56 0.37 +1.3 61,759 - HP15_56 0.38 -1.1 61,852 - HP15_56 0.46 -1.4 61,943 + HP15_56 0.54 +0.2 61,943 + HP15_56 0.54 -1.2 61,951 - HP15_56 0.55 +0.3 61,956 - HP15_56 0.55 -0.1 61,956 - HP15_56 0.55 -0.9 61,988 + HP15_56 0.58 -0.0 61,988 + HP15_56 0.58 +1.0 61,998 + HP15_56 0.59 +2.2 62,026 - HP15_56 0.61 -0.4 62,218 - HP15_56 0.78 -0.1 62,243 + HP15_56 0.81 +1.1 62,250 + HP15_56 0.81 +1.2 62,266 + HP15_56 0.83 +1.7 62,287 + HP15_56 0.84 +0.5 62,303 - HP15_56 0.86 -0.9 62,346 - HP15_56 0.90 +1.4 62,415 - -1.0 62,481 + -0.1 62,487 - -0.8 62,516 - -2.1 62,536 - +0.8 62,577 - -0.3 62,590 - HP15_57 0.11 +0.6 62,649 - HP15_57 0.17 +2.7 62,654 - HP15_57 0.18 +0.1 62,656 + HP15_57 0.18 -1.2 62,666 + HP15_57 0.19 +1.0 62,682 + HP15_57 0.21 -0.2 62,786 + HP15_57 0.32 +0.2 62,799 - HP15_57 0.34 +0.5 62,837 + HP15_57 0.38 -0.2 62,837 + HP15_57 0.38 +1.5 62,873 - HP15_57 0.42 +0.5 62,888 - HP15_57 0.44 +1.3 62,920 - HP15_57 0.47 +2.2 63,140 + HP15_57 0.72 +1.4 63,201 + HP15_57 0.79 +1.0 63,313 - +0.6 63,343 - +0.8 63,461 - +0.3
Or see this region's nucleotide sequence