Experiment: monoculture; Experiment B, time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1013 and HP15_1014 are separated by 55 nucleotides HP15_1014 and HP15_1015 are separated by 27 nucleotides HP15_1015 and HP15_1016 are separated by 395 nucleotides HP15_1016 and HP15_1017 overlap by 4 nucleotides
HP15_1013: HP15_1013 - nitrate transport system permease protein, at 1,026,334 to 1,027,161
_1013
HP15_1014: HP15_1014 - nitrate ABC transporter, ATPase subunits C and D, at 1,027,217 to 1,028,125
_1014
HP15_1015: HP15_1015 - cyanate lyase, at 1,028,153 to 1,028,596
_1015
HP15_1016: HP15_1016 - acetolactate synthase II, small subunit-like protein, at 1,028,992 to 1,029,270
_1016
HP15_1017: HP15_1017 - acetolactate synthase, large subunit, biosynthetic type, at 1,029,267 to 1,030,967
_1017
Position (kb)
1028
1029 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1027.156 kb on + strand at 1027.190 kb on + strand at 1027.199 kb on + strand at 1027.402 kb on - strand, within HP15_1014 at 1027.507 kb on + strand, within HP15_1014 at 1027.507 kb on + strand, within HP15_1014 at 1027.713 kb on - strand, within HP15_1014 at 1028.057 kb on - strand at 1028.134 kb on - strand at 1028.134 kb on - strand at 1028.173 kb on - strand at 1028.198 kb on + strand, within HP15_1015 at 1028.214 kb on + strand, within HP15_1015 at 1028.215 kb on - strand, within HP15_1015 at 1028.303 kb on + strand, within HP15_1015 at 1028.328 kb on + strand, within HP15_1015 at 1028.375 kb on + strand, within HP15_1015 at 1028.547 kb on + strand, within HP15_1015 at 1028.547 kb on + strand, within HP15_1015 at 1028.572 kb on + strand at 1028.572 kb on + strand at 1028.634 kb on - strand at 1028.761 kb on - strand at 1028.862 kb on - strand at 1028.983 kb on + strand at 1028.986 kb on - strand at 1029.115 kb on + strand, within HP15_1016 at 1029.179 kb on + strand, within HP15_1016 at 1029.231 kb on + strand, within HP15_1016 at 1029.259 kb on - strand at 1029.365 kb on + strand at 1029.435 kb on + strand at 1029.435 kb on + strand at 1029.538 kb on + strand, within HP15_1017
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 3 remove 1,027,156 + -0.7 1,027,190 + +0.4 1,027,199 + +0.6 1,027,402 - HP15_1014 0.20 +2.2 1,027,507 + HP15_1014 0.32 -2.6 1,027,507 + HP15_1014 0.32 +1.0 1,027,713 - HP15_1014 0.55 +0.3 1,028,057 - -0.3 1,028,134 - -0.7 1,028,134 - +0.4 1,028,173 - -0.1 1,028,198 + HP15_1015 0.10 +1.5 1,028,214 + HP15_1015 0.14 +0.5 1,028,215 - HP15_1015 0.14 -0.8 1,028,303 + HP15_1015 0.34 +0.1 1,028,328 + HP15_1015 0.39 -1.3 1,028,375 + HP15_1015 0.50 -1.5 1,028,547 + HP15_1015 0.89 -0.4 1,028,547 + HP15_1015 0.89 +1.7 1,028,572 + +0.3 1,028,572 + -1.7 1,028,634 - -0.8 1,028,761 - -0.0 1,028,862 - -0.8 1,028,983 + +0.7 1,028,986 - -1.4 1,029,115 + HP15_1016 0.44 +0.7 1,029,179 + HP15_1016 0.67 +0.7 1,029,231 + HP15_1016 0.86 -0.7 1,029,259 - -0.7 1,029,365 + -0.4 1,029,435 + -0.1 1,029,435 + -0.5 1,029,538 + HP15_1017 0.16 -1.4
Or see this region's nucleotide sequence