Experiment: monoculture; Experiment A, time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_2986 and HP15_2987 are separated by 6 nucleotides HP15_2987 and HP15_2988 are separated by 13 nucleotides HP15_2988 and HP15_2989 are separated by 39 nucleotides HP15_2989 and HP15_2990 are separated by 0 nucleotides
HP15_2986: HP15_2986 - FeS assembly protein SufD, at 3,129,877 to 3,131,163
_2986
HP15_2987: HP15_2987 - cysteine desulfurase, SufS subfamily, at 3,131,170 to 3,132,423
_2987
HP15_2988: HP15_2988 - FeS cluster insertion, at 3,132,437 to 3,132,790
_2988
HP15_2989: HP15_2989 - metal-sulfur cluster biosynthetic enzyme, at 3,132,830 to 3,133,372
_2989
HP15_2990: HP15_2990 - SufE protein probably involved in Fe-S center assembly, at 3,133,373 to 3,133,843
_2990
Position (kb)
3131
3132
3133 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3131.113 kb on + strand at 3131.113 kb on + strand at 3131.113 kb on + strand at 3131.113 kb on + strand at 3131.113 kb on + strand at 3132.396 kb on + strand at 3132.876 kb on + strand at 3132.918 kb on + strand, within HP15_2989 at 3132.978 kb on - strand, within HP15_2989 at 3132.983 kb on - strand, within HP15_2989 at 3133.088 kb on + strand, within HP15_2989 at 3133.301 kb on - strand, within HP15_2989 at 3133.313 kb on - strand, within HP15_2989
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment A, time point 3 remove 3,131,113 + -1.2 3,131,113 + +0.4 3,131,113 + -1.6 3,131,113 + -1.0 3,131,113 + -0.6 3,132,396 + +0.6 3,132,876 + -2.1 3,132,918 + HP15_2989 0.16 -2.0 3,132,978 - HP15_2989 0.27 -1.0 3,132,983 - HP15_2989 0.28 -2.3 3,133,088 + HP15_2989 0.48 -4.0 3,133,301 - HP15_2989 0.87 -4.5 3,133,313 - HP15_2989 0.89 -3.5
Or see this region's nucleotide sequence