Strain Fitness in Methanococcus maripaludis S2 around MMP_RS08225

Experiment: McCas_H2_complete Benzethonium chloride 0.00625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS08215 and MMP_RS08220 are separated by 15 nucleotidesMMP_RS08220 and MMP_RS08225 are separated by 30 nucleotidesMMP_RS08225 and MMP_RS08230 are separated by 27 nucleotidesMMP_RS08230 and MMP_RS08235 are separated by 106 nucleotides MMP_RS08215: MMP_RS08215 - DUF483 domain-containing protein, at 1,546,728 to 1,547,282 _RS08215 MMP_RS08220: MMP_RS08220 - TrkA family potassium uptake protein, at 1,547,298 to 1,547,963 _RS08220 MMP_RS08225: MMP_RS08225 - RimK family alpha-L-glutamate ligase, at 1,547,994 to 1,548,800 _RS08225 MMP_RS08230: MMP_RS08230 - 50S ribosomal protein L11 methyltransferase, at 1,548,828 to 1,549,610 _RS08230 MMP_RS08235: MMP_RS08235 - hypothetical protein, at 1,549,717 to 1,549,944 _RS08235 Position (kb) 1547 1548 1549Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5 6 7 8 9 10 11 12at 1547.033 kb on + strand, within MMP_RS08215at 1547.033 kb on + strand, within MMP_RS08215at 1547.064 kb on + strand, within MMP_RS08215at 1547.374 kb on - strandat 1547.500 kb on - strandat 1547.501 kb on + strandat 1547.996 kb on + strandat 1548.000 kb on - strandat 1548.009 kb on + strandat 1548.017 kb on + strandat 1548.062 kb on - strandat 1548.088 kb on + strand, within MMP_RS08225at 1548.310 kb on - strand, within MMP_RS08225at 1548.311 kb on + strand, within MMP_RS08225at 1548.311 kb on + strand, within MMP_RS08225at 1548.418 kb on - strand, within MMP_RS08225at 1548.418 kb on - strand, within MMP_RS08225at 1548.418 kb on - strand, within MMP_RS08225at 1548.418 kb on - strand, within MMP_RS08225at 1548.419 kb on + strand, within MMP_RS08225at 1548.419 kb on + strand, within MMP_RS08225at 1548.419 kb on + strand, within MMP_RS08225at 1548.419 kb on + strand, within MMP_RS08225at 1548.420 kb on - strand, within MMP_RS08225at 1548.420 kb on - strand, within MMP_RS08225at 1548.430 kb on + strand, within MMP_RS08225at 1548.532 kb on + strand, within MMP_RS08225at 1549.088 kb on - strand, within MMP_RS08230at 1549.144 kb on - strand, within MMP_RS08230at 1549.145 kb on + strand, within MMP_RS08230at 1549.287 kb on - strand, within MMP_RS08230at 1549.303 kb on + strand, within MMP_RS08230at 1549.333 kb on - strand, within MMP_RS08230at 1549.351 kb on + strand, within MMP_RS08230at 1549.369 kb on - strand, within MMP_RS08230at 1549.370 kb on + strand, within MMP_RS08230at 1549.398 kb on + strand, within MMP_RS08230at 1549.538 kb on - strandat 1549.563 kb on - strandat 1549.576 kb on - strandat 1549.576 kb on - strandat 1549.577 kb on + strandat 1549.577 kb on + strandat 1549.656 kb on + strandat 1549.657 kb on - strandat 1549.657 kb on - strandat 1549.658 kb on + strandat 1549.658 kb on + strandat 1549.658 kb on + strandat 1549.658 kb on + strandat 1549.658 kb on + strandat 1549.714 kb on - strandat 1549.750 kb on + strand, within MMP_RS08235

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Per-strain Table

Position Strand Gene LocusTag Fraction McCas_H2_complete Benzethonium chloride 0.00625 mM
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1,547,033 + MMP_RS08215 0.55 -0.7
1,547,033 + MMP_RS08215 0.55 -0.8
1,547,064 + MMP_RS08215 0.61 -1.1
1,547,374 - +0.7
1,547,500 - -0.2
1,547,501 + +0.9
1,547,996 + +1.1
1,548,000 - +0.6
1,548,009 + -0.1
1,548,017 + -0.4
1,548,062 - -2.0
1,548,088 + MMP_RS08225 0.12 -1.7
1,548,310 - MMP_RS08225 0.39 +0.1
1,548,311 + MMP_RS08225 0.39 -2.1
1,548,311 + MMP_RS08225 0.39 -0.1
1,548,418 - MMP_RS08225 0.53 +0.2
1,548,418 - MMP_RS08225 0.53 -0.0
1,548,418 - MMP_RS08225 0.53 -1.1
1,548,418 - MMP_RS08225 0.53 -0.1
1,548,419 + MMP_RS08225 0.53 -0.2
1,548,419 + MMP_RS08225 0.53 -0.5
1,548,419 + MMP_RS08225 0.53 -1.3
1,548,419 + MMP_RS08225 0.53 +0.3
1,548,420 - MMP_RS08225 0.53 +0.1
1,548,420 - MMP_RS08225 0.53 +0.7
1,548,430 + MMP_RS08225 0.54 -1.5
1,548,532 + MMP_RS08225 0.67 -0.8
1,549,088 - MMP_RS08230 0.33 -1.6
1,549,144 - MMP_RS08230 0.40 -1.1
1,549,145 + MMP_RS08230 0.40 +0.5
1,549,287 - MMP_RS08230 0.59 -1.9
1,549,303 + MMP_RS08230 0.61 -0.3
1,549,333 - MMP_RS08230 0.64 -1.3
1,549,351 + MMP_RS08230 0.67 +0.4
1,549,369 - MMP_RS08230 0.69 +0.4
1,549,370 + MMP_RS08230 0.69 -0.3
1,549,398 + MMP_RS08230 0.73 -0.3
1,549,538 - -0.4
1,549,563 - +0.4
1,549,576 - -0.4
1,549,576 - +0.2
1,549,577 + -0.8
1,549,577 + -0.2
1,549,656 + -1.9
1,549,657 - -1.8
1,549,657 - +0.8
1,549,658 + +0.4
1,549,658 + -0.1
1,549,658 + +0.3
1,549,658 + -1.0
1,549,658 + +0.2
1,549,714 - -0.5
1,549,750 + MMP_RS08235 0.14 +12.5

Or see this region's nucleotide sequence