Experiment: McCas_H2_complete no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00815 and MMP_RS00820 are separated by 18 nucleotides MMP_RS00820 and MMP_RS00825 are separated by 27 nucleotides MMP_RS00825 and MMP_RS00830 are separated by 15 nucleotides
MMP_RS00815: MMP_RS00815 - thiamine pyrophosphate-binding protein, at 151,531 to 153,036
_RS00815
MMP_RS00820: MMP_RS00820 - DUF2117 domain-containing protein, at 153,055 to 154,170
_RS00820
MMP_RS00825: MMP_RS00825 - fumarylacetoacetate hydrolase family protein, at 154,198 to 154,806
_RS00825
MMP_RS00830: MMP_RS00830 - purine phosphoribosyltransferase family protein, at 154,822 to 155,379
_RS00830
Position (kb)
153
154
155 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 152.128 kb on - strand at 152.140 kb on + strand at 152.154 kb on - strand at 152.165 kb on + strand at 152.179 kb on - strand at 152.179 kb on - strand at 152.185 kb on + strand at 152.185 kb on + strand at 152.185 kb on + strand at 152.420 kb on - strand at 152.426 kb on + strand at 152.438 kb on + strand at 152.613 kb on + strand at 152.639 kb on - strand at 152.643 kb on - strand at 152.903 kb on + strand at 153.213 kb on - strand at 153.327 kb on - strand at 153.345 kb on - strand at 153.355 kb on - strand at 153.562 kb on + strand at 153.562 kb on + strand at 153.574 kb on + strand at 153.592 kb on - strand at 154.681 kb on + strand, within MMP_RS00825 at 154.684 kb on - strand, within MMP_RS00825 at 154.684 kb on - strand, within MMP_RS00825 at 154.711 kb on - strand, within MMP_RS00825 at 154.740 kb on - strand, within MMP_RS00825 at 154.798 kb on - strand at 155.145 kb on + strand, within MMP_RS00830
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_H2_complete no stress control remove 152,128 - -1.2 152,140 + -0.9 152,154 - -0.4 152,165 + -1.7 152,179 - -2.0 152,179 - -2.1 152,185 + -2.2 152,185 + -2.9 152,185 + -1.2 152,420 - -1.5 152,426 + -0.7 152,438 + -0.7 152,613 + -1.1 152,639 - -2.0 152,643 - -0.7 152,903 + -3.1 153,213 - -1.8 153,327 - -1.7 153,345 - -1.1 153,355 - -2.3 153,562 + -2.6 153,562 + -2.3 153,574 + -1.7 153,592 - -1.4 154,681 + MMP_RS00825 0.79 +0.2 154,684 - MMP_RS00825 0.80 +1.2 154,684 - MMP_RS00825 0.80 -0.2 154,711 - MMP_RS00825 0.84 +2.1 154,740 - MMP_RS00825 0.89 -0.5 154,798 - -0.7 155,145 + MMP_RS00830 0.58 -0.4
Or see this region's nucleotide sequence