Experiment: McCas_H2_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08470 and MMP_RS08475 overlap by 4 nucleotides MMP_RS08475 and MMP_RS08480 overlap by 10 nucleotides MMP_RS08480 and MMP_RS08485 are separated by 82 nucleotides
MMP_RS08470: MMP_RS08470 - ATP-binding cassette domain-containing protein, at 1,589,977 to 1,591,038
_RS08470
MMP_RS08475: MMP_RS08475 - ABC transporter permease, at 1,591,035 to 1,591,817
_RS08475
MMP_RS08480: MMP_RS08480 - molybdate ABC transporter substrate-binding protein, at 1,591,808 to 1,592,611
_RS08480
MMP_RS08485: MMP_RS08485 - tungstate ABC transporter substrate-binding protein WtpA, at 1,592,694 to 1,593,692
_RS08485
Position (kb)
1591
1592
1593 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1590.812 kb on + strand, within MMP_RS08470 at 1590.841 kb on - strand, within MMP_RS08470 at 1590.842 kb on + strand, within MMP_RS08470 at 1590.842 kb on + strand, within MMP_RS08470 at 1590.862 kb on - strand, within MMP_RS08470 at 1590.947 kb on + strand at 1590.950 kb on - strand at 1590.951 kb on + strand at 1590.970 kb on - strand at 1591.054 kb on - strand at 1591.093 kb on + strand at 1591.150 kb on - strand, within MMP_RS08475 at 1591.177 kb on - strand, within MMP_RS08475 at 1591.178 kb on + strand, within MMP_RS08475 at 1591.183 kb on - strand, within MMP_RS08475 at 1591.286 kb on - strand, within MMP_RS08475 at 1591.374 kb on - strand, within MMP_RS08475 at 1591.374 kb on - strand, within MMP_RS08475 at 1591.374 kb on - strand, within MMP_RS08475 at 1591.374 kb on - strand, within MMP_RS08475 at 1591.374 kb on - strand, within MMP_RS08475 at 1591.375 kb on + strand, within MMP_RS08475 at 1591.515 kb on - strand, within MMP_RS08475 at 1591.728 kb on - strand, within MMP_RS08475 at 1591.729 kb on + strand, within MMP_RS08475 at 1591.729 kb on + strand, within MMP_RS08475 at 1591.733 kb on - strand, within MMP_RS08475 at 1591.755 kb on - strand at 1591.865 kb on - strand at 1591.971 kb on + strand, within MMP_RS08480 at 1592.000 kb on + strand, within MMP_RS08480 at 1592.050 kb on + strand, within MMP_RS08480 at 1592.050 kb on + strand, within MMP_RS08480 at 1592.130 kb on + strand, within MMP_RS08480 at 1592.131 kb on - strand, within MMP_RS08480 at 1592.271 kb on + strand, within MMP_RS08480 at 1592.271 kb on + strand, within MMP_RS08480 at 1592.291 kb on - strand, within MMP_RS08480 at 1592.299 kb on + strand, within MMP_RS08480 at 1592.349 kb on + strand, within MMP_RS08480 at 1592.403 kb on - strand, within MMP_RS08480 at 1592.451 kb on + strand, within MMP_RS08480 at 1592.451 kb on + strand, within MMP_RS08480 at 1592.461 kb on - strand, within MMP_RS08480 at 1592.592 kb on + strand at 1592.636 kb on + strand at 1592.800 kb on + strand, within MMP_RS08485 at 1592.908 kb on + strand, within MMP_RS08485 at 1592.984 kb on + strand, within MMP_RS08485 at 1593.016 kb on - strand, within MMP_RS08485 at 1593.021 kb on + strand, within MMP_RS08485 at 1593.028 kb on - strand, within MMP_RS08485 at 1593.035 kb on + strand, within MMP_RS08485 at 1593.087 kb on - strand, within MMP_RS08485 at 1593.123 kb on - strand, within MMP_RS08485 at 1593.133 kb on - strand, within MMP_RS08485 at 1593.133 kb on - strand, within MMP_RS08485 at 1593.171 kb on + strand, within MMP_RS08485 at 1593.194 kb on + strand, within MMP_RS08485 at 1593.287 kb on + strand, within MMP_RS08485 at 1593.436 kb on + strand, within MMP_RS08485 at 1593.436 kb on + strand, within MMP_RS08485 at 1593.548 kb on + strand, within MMP_RS08485 at 1593.560 kb on + strand, within MMP_RS08485 at 1593.610 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_H2_complete Antimycin A 0.01 mM remove 1,590,812 + MMP_RS08470 0.79 +1.7 1,590,841 - MMP_RS08470 0.81 +2.4 1,590,842 + MMP_RS08470 0.81 -0.2 1,590,842 + MMP_RS08470 0.81 +0.1 1,590,862 - MMP_RS08470 0.83 -0.5 1,590,947 + +0.3 1,590,950 - -0.8 1,590,951 + -0.3 1,590,970 - +2.1 1,591,054 - +0.9 1,591,093 + +0.7 1,591,150 - MMP_RS08475 0.15 -1.6 1,591,177 - MMP_RS08475 0.18 -0.1 1,591,178 + MMP_RS08475 0.18 -0.2 1,591,183 - MMP_RS08475 0.19 +0.5 1,591,286 - MMP_RS08475 0.32 +1.0 1,591,374 - MMP_RS08475 0.43 +0.2 1,591,374 - MMP_RS08475 0.43 +1.6 1,591,374 - MMP_RS08475 0.43 +1.8 1,591,374 - MMP_RS08475 0.43 +0.5 1,591,374 - MMP_RS08475 0.43 -1.9 1,591,375 + MMP_RS08475 0.43 -0.1 1,591,515 - MMP_RS08475 0.61 +1.1 1,591,728 - MMP_RS08475 0.89 +4.5 1,591,729 + MMP_RS08475 0.89 -1.1 1,591,729 + MMP_RS08475 0.89 -2.2 1,591,733 - MMP_RS08475 0.89 +0.5 1,591,755 - +2.9 1,591,865 - +0.4 1,591,971 + MMP_RS08480 0.20 +0.5 1,592,000 + MMP_RS08480 0.24 +1.3 1,592,050 + MMP_RS08480 0.30 +1.0 1,592,050 + MMP_RS08480 0.30 +0.3 1,592,130 + MMP_RS08480 0.40 +1.9 1,592,131 - MMP_RS08480 0.40 +1.7 1,592,271 + MMP_RS08480 0.58 +1.4 1,592,271 + MMP_RS08480 0.58 -1.4 1,592,291 - MMP_RS08480 0.60 +3.8 1,592,299 + MMP_RS08480 0.61 +1.6 1,592,349 + MMP_RS08480 0.67 +1.0 1,592,403 - MMP_RS08480 0.74 +1.8 1,592,451 + MMP_RS08480 0.80 -0.3 1,592,451 + MMP_RS08480 0.80 +2.7 1,592,461 - MMP_RS08480 0.81 +2.5 1,592,592 + +2.6 1,592,636 + +1.3 1,592,800 + MMP_RS08485 0.11 +3.2 1,592,908 + MMP_RS08485 0.21 +0.8 1,592,984 + MMP_RS08485 0.29 +2.7 1,593,016 - MMP_RS08485 0.32 +1.0 1,593,021 + MMP_RS08485 0.33 +0.1 1,593,028 - MMP_RS08485 0.33 +0.8 1,593,035 + MMP_RS08485 0.34 -0.1 1,593,087 - MMP_RS08485 0.39 -0.5 1,593,123 - MMP_RS08485 0.43 +3.3 1,593,133 - MMP_RS08485 0.44 +0.8 1,593,133 - MMP_RS08485 0.44 +1.8 1,593,171 + MMP_RS08485 0.48 +1.2 1,593,194 + MMP_RS08485 0.50 -0.2 1,593,287 + MMP_RS08485 0.59 +0.3 1,593,436 + MMP_RS08485 0.74 +1.1 1,593,436 + MMP_RS08485 0.74 -0.9 1,593,548 + MMP_RS08485 0.85 +1.2 1,593,560 + MMP_RS08485 0.87 +0.6 1,593,610 + -1.0
Or see this region's nucleotide sequence