Experiment: McCas_H2_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06125 and MMP_RS06130 are separated by 68 nucleotides MMP_RS06130 and MMP_RS06135 are separated by 190 nucleotides MMP_RS06135 and MMP_RS06140 are separated by 119 nucleotides
MMP_RS06125: MMP_RS06125 - ribose-5-phosphate isomerase RpiA, at 1,171,728 to 1,172,447
_RS06125
MMP_RS06130: MMP_RS06130 - peptidylprolyl isomerase, at 1,172,516 to 1,172,968
_RS06130
MMP_RS06135: MMP_RS06135 - methenyltetrahydromethanopterin cyclohydrolase, at 1,173,159 to 1,174,130
_RS06135
MMP_RS06140: MMP_RS06140 - radical SAM protein, at 1,174,250 to 1,175,365
_RS06140
Position (kb)
1173
1174
1175 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1172.164 kb on + strand at 1172.220 kb on + strand at 1172.249 kb on - strand at 1172.249 kb on - strand at 1172.250 kb on + strand at 1172.264 kb on - strand at 1172.265 kb on + strand at 1172.436 kb on + strand at 1172.495 kb on + strand at 1172.643 kb on + strand, within MMP_RS06130 at 1172.756 kb on - strand, within MMP_RS06130 at 1172.844 kb on - strand, within MMP_RS06130 at 1172.844 kb on - strand, within MMP_RS06130 at 1172.845 kb on + strand, within MMP_RS06130 at 1172.882 kb on - strand, within MMP_RS06130 at 1172.951 kb on + strand at 1172.982 kb on - strand at 1173.017 kb on - strand at 1173.017 kb on - strand at 1173.017 kb on - strand at 1173.021 kb on + strand at 1173.023 kb on + strand at 1173.023 kb on + strand at 1173.023 kb on + strand at 1173.023 kb on + strand at 1173.023 kb on + strand at 1173.023 kb on + strand at 1173.023 kb on + strand at 1173.023 kb on + strand at 1173.024 kb on - strand at 1173.024 kb on - strand at 1173.025 kb on + strand at 1173.026 kb on - strand at 1173.027 kb on + strand at 1173.049 kb on - strand at 1173.050 kb on + strand at 1173.054 kb on - strand at 1173.137 kb on + strand at 1173.165 kb on + strand at 1173.262 kb on - strand at 1173.397 kb on - strand at 1173.920 kb on - strand at 1173.967 kb on - strand at 1174.096 kb on - strand at 1174.128 kb on - strand at 1174.139 kb on + strand at 1174.145 kb on - strand at 1174.149 kb on - strand at 1174.152 kb on + strand at 1174.156 kb on + strand at 1174.157 kb on - strand at 1174.157 kb on - strand at 1174.178 kb on - strand at 1174.178 kb on - strand at 1174.327 kb on + strand at 1174.329 kb on + strand at 1174.358 kb on - strand at 1174.526 kb on - strand, within MMP_RS06140 at 1174.794 kb on - strand, within MMP_RS06140 at 1174.906 kb on - strand, within MMP_RS06140 at 1174.907 kb on + strand, within MMP_RS06140 at 1174.949 kb on + strand, within MMP_RS06140 at 1175.091 kb on - strand, within MMP_RS06140
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_H2_complete Antimycin A 0.01 mM remove 1,172,164 + -1.4 1,172,220 + -1.3 1,172,249 - -2.8 1,172,249 - +0.0 1,172,250 + -0.7 1,172,264 - +3.0 1,172,265 + -0.7 1,172,436 + -0.6 1,172,495 + -0.5 1,172,643 + MMP_RS06130 0.28 -0.6 1,172,756 - MMP_RS06130 0.53 +0.1 1,172,844 - MMP_RS06130 0.72 -1.8 1,172,844 - MMP_RS06130 0.72 -1.5 1,172,845 + MMP_RS06130 0.73 -1.8 1,172,882 - MMP_RS06130 0.81 -0.9 1,172,951 + -1.6 1,172,982 - +0.5 1,173,017 - +1.1 1,173,017 - +0.4 1,173,017 - -0.1 1,173,021 + -0.6 1,173,023 + +1.6 1,173,023 + -1.4 1,173,023 + -1.0 1,173,023 + +0.6 1,173,023 + -1.7 1,173,023 + +0.1 1,173,023 + -0.0 1,173,023 + -2.0 1,173,024 - +0.1 1,173,024 - -0.6 1,173,025 + -0.8 1,173,026 - +1.2 1,173,027 + +0.7 1,173,049 - +0.0 1,173,050 + -0.7 1,173,054 - -1.4 1,173,137 + -0.2 1,173,165 + -1.7 1,173,262 - -0.3 1,173,397 - -3.3 1,173,920 - -0.3 1,173,967 - -1.7 1,174,096 - -3.4 1,174,128 - +0.8 1,174,139 + -0.4 1,174,145 - +0.9 1,174,149 - -0.7 1,174,152 + +0.7 1,174,156 + +0.9 1,174,157 - +0.4 1,174,157 - +0.8 1,174,178 - +0.4 1,174,178 - -2.9 1,174,327 + +1.0 1,174,329 + -0.8 1,174,358 - -0.3 1,174,526 - MMP_RS06140 0.25 -1.1 1,174,794 - MMP_RS06140 0.49 +2.0 1,174,906 - MMP_RS06140 0.59 -0.8 1,174,907 + MMP_RS06140 0.59 +1.2 1,174,949 + MMP_RS06140 0.63 +3.9 1,175,091 - MMP_RS06140 0.75 +0.3
Or see this region's nucleotide sequence