Experiment: McCas_H2_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05120 and MMP_RS05125 are separated by 328 nucleotides MMP_RS05125 and MMP_RS05130 are separated by 379 nucleotides
MMP_RS05120: MMP_RS05120 - DNA topoisomerase VI subunit B, at 975,268 to 977,262
_RS05120
MMP_RS05125: MMP_RS05125 - D-aminoacyl-tRNA deacylase, at 977,591 to 978,355
_RS05125
MMP_RS05130: MMP_RS05130 - DUF2226 domain-containing protein, at 978,735 to 980,321
_RS05130
Position (kb)
977
978
979 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 976.703 kb on - strand at 977.280 kb on - strand at 977.283 kb on + strand at 977.287 kb on - strand at 977.371 kb on - strand at 977.371 kb on - strand at 977.372 kb on + strand at 977.372 kb on + strand at 977.372 kb on + strand at 977.384 kb on + strand at 977.385 kb on - strand at 977.385 kb on - strand at 977.385 kb on - strand at 977.386 kb on + strand at 977.386 kb on + strand at 977.386 kb on + strand at 977.387 kb on - strand at 977.387 kb on - strand at 977.387 kb on - strand at 977.388 kb on + strand at 977.388 kb on + strand at 977.388 kb on + strand at 977.389 kb on - strand at 977.425 kb on + strand at 977.427 kb on - strand at 977.436 kb on - strand at 977.450 kb on + strand at 977.503 kb on - strand at 977.527 kb on - strand at 977.528 kb on + strand at 977.529 kb on - strand at 977.530 kb on + strand at 977.534 kb on + strand at 977.604 kb on - strand at 977.673 kb on - strand, within MMP_RS05125 at 977.673 kb on - strand, within MMP_RS05125 at 977.708 kb on - strand, within MMP_RS05125 at 977.735 kb on - strand, within MMP_RS05125 at 977.736 kb on + strand, within MMP_RS05125 at 977.736 kb on + strand, within MMP_RS05125 at 977.736 kb on + strand, within MMP_RS05125 at 977.736 kb on + strand, within MMP_RS05125 at 977.737 kb on - strand, within MMP_RS05125 at 977.739 kb on - strand, within MMP_RS05125 at 977.740 kb on + strand, within MMP_RS05125 at 977.740 kb on + strand, within MMP_RS05125 at 977.787 kb on - strand, within MMP_RS05125 at 977.788 kb on + strand, within MMP_RS05125 at 977.791 kb on - strand, within MMP_RS05125 at 977.791 kb on - strand, within MMP_RS05125 at 977.791 kb on - strand, within MMP_RS05125 at 977.792 kb on + strand, within MMP_RS05125 at 977.952 kb on - strand, within MMP_RS05125 at 978.072 kb on - strand, within MMP_RS05125 at 978.137 kb on - strand, within MMP_RS05125 at 978.193 kb on + strand, within MMP_RS05125 at 978.214 kb on + strand, within MMP_RS05125 at 978.224 kb on + strand, within MMP_RS05125 at 978.299 kb on + strand at 978.389 kb on - strand at 978.390 kb on + strand at 978.393 kb on - strand at 978.394 kb on + strand at 978.394 kb on + strand at 978.396 kb on - strand at 978.408 kb on - strand at 978.409 kb on + strand at 978.423 kb on - strand at 978.423 kb on - strand at 978.423 kb on - strand at 978.424 kb on + strand at 978.424 kb on + strand at 978.435 kb on - strand at 978.439 kb on - strand at 978.439 kb on - strand at 978.446 kb on - strand at 978.447 kb on + strand at 978.630 kb on - strand at 978.630 kb on - strand at 978.631 kb on + strand at 978.633 kb on + strand at 978.702 kb on - strand at 978.703 kb on + strand at 978.721 kb on - strand at 978.745 kb on - strand at 978.745 kb on - strand at 978.745 kb on - strand at 978.745 kb on - strand at 978.746 kb on + strand at 978.746 kb on + strand at 978.777 kb on - strand at 978.874 kb on - strand at 978.882 kb on + strand at 978.909 kb on - strand, within MMP_RS05130 at 978.944 kb on + strand, within MMP_RS05130 at 979.233 kb on - strand, within MMP_RS05130 at 979.234 kb on + strand, within MMP_RS05130 at 979.286 kb on - strand, within MMP_RS05130 at 979.305 kb on + strand, within MMP_RS05130 at 979.330 kb on - strand, within MMP_RS05130 at 979.331 kb on + strand, within MMP_RS05130
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_H2_complete Antimycin A 0.01 mM remove 976,703 - -2.1 977,280 - -3.1 977,283 + -0.7 977,287 - -2.0 977,371 - -0.8 977,371 - -0.4 977,372 + +0.5 977,372 + +0.7 977,372 + +0.1 977,384 + +0.0 977,385 - -1.6 977,385 - -0.7 977,385 - +0.1 977,386 + +0.2 977,386 + -1.8 977,386 + +0.4 977,387 - +2.2 977,387 - +0.2 977,387 - -1.4 977,388 + -1.3 977,388 + -1.5 977,388 + +0.6 977,389 - +1.2 977,425 + +1.1 977,427 - -0.3 977,436 - +0.2 977,450 + -1.6 977,503 - +0.2 977,527 - -0.3 977,528 + +0.7 977,529 - -1.6 977,530 + -0.3 977,534 + +0.3 977,604 - +1.6 977,673 - MMP_RS05125 0.11 -0.8 977,673 - MMP_RS05125 0.11 -1.0 977,708 - MMP_RS05125 0.15 -0.8 977,735 - MMP_RS05125 0.19 -1.3 977,736 + MMP_RS05125 0.19 +0.5 977,736 + MMP_RS05125 0.19 +0.1 977,736 + MMP_RS05125 0.19 -0.2 977,736 + MMP_RS05125 0.19 -0.9 977,737 - MMP_RS05125 0.19 +0.9 977,739 - MMP_RS05125 0.19 -0.1 977,740 + MMP_RS05125 0.19 -0.6 977,740 + MMP_RS05125 0.19 -3.2 977,787 - MMP_RS05125 0.26 -0.0 977,788 + MMP_RS05125 0.26 -2.0 977,791 - MMP_RS05125 0.26 +0.1 977,791 - MMP_RS05125 0.26 -0.2 977,791 - MMP_RS05125 0.26 +0.3 977,792 + MMP_RS05125 0.26 -0.4 977,952 - MMP_RS05125 0.47 +1.2 978,072 - MMP_RS05125 0.63 -1.2 978,137 - MMP_RS05125 0.71 -0.8 978,193 + MMP_RS05125 0.79 -1.5 978,214 + MMP_RS05125 0.81 -1.3 978,224 + MMP_RS05125 0.83 +1.3 978,299 + -0.4 978,389 - +0.5 978,390 + +0.7 978,393 - -0.1 978,394 + -1.4 978,394 + -0.4 978,396 - +0.5 978,408 - -1.0 978,409 + -0.7 978,423 - -1.8 978,423 - -0.3 978,423 - -0.3 978,424 + +1.1 978,424 + +0.6 978,435 - -0.1 978,439 - -0.7 978,439 - +0.5 978,446 - -0.7 978,447 + +0.7 978,630 - -0.5 978,630 - -0.2 978,631 + -0.4 978,633 + -1.3 978,702 - -0.7 978,703 + -0.4 978,721 - -1.4 978,745 - -0.7 978,745 - -0.5 978,745 - -1.1 978,745 - -1.4 978,746 + -1.6 978,746 + -0.5 978,777 - -1.2 978,874 - -1.7 978,882 + -1.4 978,909 - MMP_RS05130 0.11 -1.5 978,944 + MMP_RS05130 0.13 -0.4 979,233 - MMP_RS05130 0.31 -1.1 979,234 + MMP_RS05130 0.31 -0.8 979,286 - MMP_RS05130 0.35 -0.4 979,305 + MMP_RS05130 0.36 -1.1 979,330 - MMP_RS05130 0.37 -1.2 979,331 + MMP_RS05130 0.38 -1.7
Or see this region's nucleotide sequence