Experiment: McCas_H2_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05005 and MMP_RS05010 are separated by 256 nucleotides MMP_RS05010 and MMP_RS05015 are separated by 18 nucleotides MMP_RS05015 and MMP_RS05020 are separated by 33 nucleotides MMP_RS05020 and MMP_RS05025 are separated by 28 nucleotides
MMP_RS05005: MMP_RS05005 - uroporphyrinogen-III C-methyltransferase, at 953,174 to 953,893
_RS05005
MMP_RS05010: MMP_RS05010 - KEOPS complex subunit Cgi121, at 954,150 to 954,581
_RS05010
MMP_RS05015: MMP_RS05015 - histidinol dehydrogenase, at 954,600 to 955,874
_RS05015
MMP_RS05020: MMP_RS05020 - hypothetical protein, at 955,908 to 956,324
_RS05020
MMP_RS05025: MMP_RS05025 - ATP-dependent DNA ligase, at 956,353 to 958,074
_RS05025
Position (kb)
954
955
956 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 953.924 kb on - strand at 953.925 kb on + strand at 953.925 kb on + strand at 953.934 kb on - strand at 953.934 kb on - strand at 953.935 kb on + strand at 953.963 kb on + strand at 953.967 kb on + strand at 953.968 kb on - strand at 953.969 kb on + strand at 953.988 kb on - strand at 953.988 kb on - strand at 953.989 kb on + strand at 954.022 kb on + strand at 954.046 kb on + strand at 954.049 kb on - strand at 954.050 kb on + strand at 954.050 kb on + strand at 954.052 kb on + strand at 954.061 kb on - strand at 954.069 kb on - strand at 954.069 kb on - strand at 954.071 kb on - strand at 954.074 kb on - strand at 954.080 kb on + strand at 954.138 kb on + strand at 954.157 kb on - strand at 954.268 kb on - strand at 954.269 kb on + strand at 954.271 kb on + strand at 954.294 kb on + strand at 954.322 kb on - strand at 954.650 kb on + strand at 954.791 kb on + strand at 954.839 kb on + strand at 954.979 kb on + strand at 955.060 kb on - strand at 955.394 kb on - strand at 955.394 kb on - strand at 955.401 kb on + strand at 955.554 kb on - strand at 955.927 kb on - strand at 955.932 kb on + strand at 956.053 kb on - strand, within MMP_RS05020 at 956.131 kb on - strand, within MMP_RS05020 at 956.174 kb on + strand, within MMP_RS05020 at 956.281 kb on - strand, within MMP_RS05020 at 956.281 kb on - strand, within MMP_RS05020 at 956.299 kb on - strand at 956.661 kb on - strand at 956.695 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_H2_complete Antimycin A 0.01 mM remove 953,924 - -1.0 953,925 + -3.0 953,925 + -2.3 953,934 - +0.2 953,934 - -1.0 953,935 + -1.9 953,963 + -0.7 953,967 + +1.0 953,968 - +0.6 953,969 + +1.5 953,988 - -0.3 953,988 - +0.6 953,989 + +2.7 954,022 + +1.9 954,046 + +1.7 954,049 - -1.4 954,050 + -1.9 954,050 + +0.9 954,052 + -1.9 954,061 - -1.3 954,069 - -3.4 954,069 - -3.1 954,071 - -3.4 954,074 - -2.9 954,080 + +1.3 954,138 + -3.5 954,157 - -1.4 954,268 - -0.8 954,269 + -1.1 954,271 + -2.8 954,294 + -3.9 954,322 - -0.6 954,650 + -0.5 954,791 + -3.5 954,839 + -2.0 954,979 + -2.0 955,060 - -0.2 955,394 - -0.8 955,394 - -1.1 955,401 + -1.0 955,554 - -2.0 955,927 - -0.2 955,932 + +2.0 956,053 - MMP_RS05020 0.35 +0.5 956,131 - MMP_RS05020 0.53 +1.9 956,174 + MMP_RS05020 0.64 -0.1 956,281 - MMP_RS05020 0.89 -1.4 956,281 - MMP_RS05020 0.89 -1.4 956,299 - +0.3 956,661 - -2.0 956,695 + -1.4
Or see this region's nucleotide sequence