Experiment: McCas_H2_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04955 and MMP_RS04960 are separated by 1 nucleotides MMP_RS04960 and MMP_RS04965 are separated by 19 nucleotides MMP_RS04965 and MMP_RS04970 are separated by 16 nucleotides
MMP_RS04955: MMP_RS04955 - DNA topoisomerase I, at 943,765 to 946,236
_RS04955
MMP_RS04960: MMP_RS04960 - cytochrome c biogenesis CcdA family protein, at 946,238 to 946,891
_RS04960
MMP_RS04965: MMP_RS04965 - thioredoxin family protein, at 946,911 to 947,258
_RS04965
MMP_RS04970: MMP_RS04970 - F420-dependent thioredoxin reductase, at 947,275 to 948,177
_RS04970
Position (kb)
946
947
948 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 945.976 kb on + strand, within MMP_RS04955 at 946.035 kb on - strand at 946.080 kb on - strand at 946.120 kb on + strand at 946.150 kb on + strand at 946.155 kb on - strand at 946.234 kb on - strand at 946.246 kb on - strand at 946.247 kb on + strand at 946.247 kb on + strand at 946.250 kb on - strand at 946.250 kb on - strand at 946.251 kb on + strand at 946.253 kb on - strand at 946.277 kb on - strand at 946.277 kb on - strand at 946.279 kb on - strand at 946.279 kb on - strand at 946.280 kb on + strand at 946.280 kb on + strand at 946.291 kb on - strand at 946.292 kb on + strand at 946.292 kb on + strand at 946.292 kb on + strand at 946.292 kb on + strand at 946.293 kb on - strand at 946.293 kb on - strand at 946.293 kb on - strand at 946.293 kb on - strand at 946.293 kb on - strand at 946.293 kb on - strand at 946.293 kb on - strand at 946.294 kb on + strand at 946.294 kb on + strand at 946.294 kb on + strand at 946.294 kb on + strand at 946.294 kb on + strand at 946.298 kb on - strand at 946.299 kb on + strand at 946.300 kb on - strand at 946.301 kb on + strand at 946.301 kb on + strand at 946.316 kb on - strand, within MMP_RS04960 at 946.328 kb on - strand, within MMP_RS04960 at 946.328 kb on - strand, within MMP_RS04960 at 946.479 kb on + strand, within MMP_RS04960 at 946.480 kb on - strand, within MMP_RS04960 at 946.480 kb on - strand, within MMP_RS04960 at 946.480 kb on - strand, within MMP_RS04960 at 946.481 kb on + strand, within MMP_RS04960 at 946.539 kb on + strand, within MMP_RS04960 at 946.600 kb on - strand, within MMP_RS04960 at 946.601 kb on + strand, within MMP_RS04960 at 946.619 kb on + strand, within MMP_RS04960 at 946.619 kb on + strand, within MMP_RS04960 at 946.622 kb on - strand, within MMP_RS04960 at 946.623 kb on + strand, within MMP_RS04960 at 946.764 kb on + strand, within MMP_RS04960 at 946.766 kb on - strand, within MMP_RS04960 at 946.917 kb on + strand at 947.070 kb on - strand, within MMP_RS04965 at 947.071 kb on + strand, within MMP_RS04965 at 947.072 kb on - strand, within MMP_RS04965 at 947.072 kb on - strand, within MMP_RS04965 at 947.072 kb on - strand, within MMP_RS04965 at 947.072 kb on - strand, within MMP_RS04965 at 947.072 kb on - strand, within MMP_RS04965 at 947.072 kb on - strand, within MMP_RS04965 at 947.072 kb on - strand, within MMP_RS04965 at 947.073 kb on + strand, within MMP_RS04965 at 947.073 kb on + strand, within MMP_RS04965 at 947.102 kb on - strand, within MMP_RS04965 at 947.201 kb on - strand, within MMP_RS04965 at 947.221 kb on + strand, within MMP_RS04965 at 947.226 kb on - strand at 947.229 kb on + strand at 947.229 kb on + strand at 947.232 kb on - strand at 947.233 kb on + strand at 947.233 kb on + strand at 947.273 kb on - strand at 947.293 kb on - strand at 947.392 kb on + strand, within MMP_RS04970 at 947.434 kb on + strand, within MMP_RS04970 at 947.488 kb on - strand, within MMP_RS04970 at 947.635 kb on - strand, within MMP_RS04970 at 947.653 kb on - strand, within MMP_RS04970 at 947.674 kb on - strand, within MMP_RS04970 at 947.675 kb on + strand, within MMP_RS04970 at 947.675 kb on + strand, within MMP_RS04970 at 947.694 kb on - strand, within MMP_RS04970 at 947.695 kb on + strand, within MMP_RS04970 at 947.706 kb on - strand, within MMP_RS04970 at 947.706 kb on - strand, within MMP_RS04970 at 947.708 kb on - strand, within MMP_RS04970 at 947.708 kb on - strand, within MMP_RS04970 at 947.708 kb on - strand, within MMP_RS04970 at 947.786 kb on + strand, within MMP_RS04970 at 947.828 kb on + strand, within MMP_RS04970 at 947.847 kb on - strand, within MMP_RS04970 at 947.847 kb on - strand, within MMP_RS04970 at 947.847 kb on - strand, within MMP_RS04970 at 947.847 kb on - strand, within MMP_RS04970 at 947.863 kb on - strand, within MMP_RS04970 at 947.863 kb on - strand, within MMP_RS04970 at 947.864 kb on + strand, within MMP_RS04970 at 947.866 kb on - strand, within MMP_RS04970 at 947.883 kb on + strand, within MMP_RS04970 at 947.925 kb on - strand, within MMP_RS04970 at 947.930 kb on + strand, within MMP_RS04970 at 948.172 kb on - strand at 948.202 kb on - strand at 948.203 kb on + strand at 948.220 kb on + strand at 948.221 kb on - strand at 948.223 kb on - strand at 948.223 kb on - strand at 948.223 kb on - strand at 948.228 kb on - strand at 948.228 kb on - strand at 948.229 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_H2_complete Antimycin A 0.01 mM remove 945,976 + MMP_RS04955 0.89 +0.3 946,035 - +1.0 946,080 - +1.2 946,120 + -0.7 946,150 + +1.1 946,155 - +0.1 946,234 - +1.1 946,246 - +0.1 946,247 + +1.2 946,247 + +1.0 946,250 - -0.7 946,250 - +0.3 946,251 + -1.5 946,253 - +0.8 946,277 - +1.9 946,277 - -0.2 946,279 - -0.1 946,279 - +0.9 946,280 + -1.8 946,280 + +3.0 946,291 - -0.7 946,292 + +0.6 946,292 + -0.9 946,292 + +0.7 946,292 + +1.1 946,293 - +2.7 946,293 - -0.7 946,293 - -0.7 946,293 - +0.8 946,293 - -0.3 946,293 - -0.0 946,293 - +0.5 946,294 + +0.1 946,294 + +1.5 946,294 + -0.6 946,294 + +0.3 946,294 + -0.6 946,298 - +1.8 946,299 + -0.4 946,300 - -1.2 946,301 + -0.1 946,301 + +1.9 946,316 - MMP_RS04960 0.12 +0.1 946,328 - MMP_RS04960 0.14 +0.6 946,328 - MMP_RS04960 0.14 +1.4 946,479 + MMP_RS04960 0.37 -1.0 946,480 - MMP_RS04960 0.37 +0.9 946,480 - MMP_RS04960 0.37 +0.3 946,480 - MMP_RS04960 0.37 +0.8 946,481 + MMP_RS04960 0.37 +0.6 946,539 + MMP_RS04960 0.46 -1.0 946,600 - MMP_RS04960 0.55 -0.0 946,601 + MMP_RS04960 0.56 +0.5 946,619 + MMP_RS04960 0.58 +0.3 946,619 + MMP_RS04960 0.58 -0.8 946,622 - MMP_RS04960 0.59 -0.0 946,623 + MMP_RS04960 0.59 +1.4 946,764 + MMP_RS04960 0.80 +0.9 946,766 - MMP_RS04960 0.81 +0.2 946,917 + +1.2 947,070 - MMP_RS04965 0.46 +0.7 947,071 + MMP_RS04965 0.46 +1.9 947,072 - MMP_RS04965 0.46 +0.5 947,072 - MMP_RS04965 0.46 +0.4 947,072 - MMP_RS04965 0.46 +1.9 947,072 - MMP_RS04965 0.46 +0.2 947,072 - MMP_RS04965 0.46 +1.8 947,072 - MMP_RS04965 0.46 +2.2 947,072 - MMP_RS04965 0.46 +0.8 947,073 + MMP_RS04965 0.47 -0.6 947,073 + MMP_RS04965 0.47 -1.6 947,102 - MMP_RS04965 0.55 +0.6 947,201 - MMP_RS04965 0.83 +1.4 947,221 + MMP_RS04965 0.89 +0.7 947,226 - +2.8 947,229 + +0.2 947,229 + +0.2 947,232 - -0.0 947,233 + +1.1 947,233 + +1.4 947,273 - -0.8 947,293 - +0.3 947,392 + MMP_RS04970 0.13 -0.7 947,434 + MMP_RS04970 0.18 +1.6 947,488 - MMP_RS04970 0.24 +0.5 947,635 - MMP_RS04970 0.40 +0.5 947,653 - MMP_RS04970 0.42 -0.5 947,674 - MMP_RS04970 0.44 -0.7 947,675 + MMP_RS04970 0.44 +1.6 947,675 + MMP_RS04970 0.44 -1.5 947,694 - MMP_RS04970 0.46 -0.9 947,695 + MMP_RS04970 0.47 +4.2 947,706 - MMP_RS04970 0.48 -1.1 947,706 - MMP_RS04970 0.48 +1.2 947,708 - MMP_RS04970 0.48 -1.4 947,708 - MMP_RS04970 0.48 -0.3 947,708 - MMP_RS04970 0.48 +0.5 947,786 + MMP_RS04970 0.57 +2.0 947,828 + MMP_RS04970 0.61 +2.1 947,847 - MMP_RS04970 0.63 -0.3 947,847 - MMP_RS04970 0.63 +0.7 947,847 - MMP_RS04970 0.63 +2.3 947,847 - MMP_RS04970 0.63 -0.5 947,863 - MMP_RS04970 0.65 +0.3 947,863 - MMP_RS04970 0.65 +0.8 947,864 + MMP_RS04970 0.65 -1.5 947,866 - MMP_RS04970 0.65 +1.1 947,883 + MMP_RS04970 0.67 -0.4 947,925 - MMP_RS04970 0.72 -0.8 947,930 + MMP_RS04970 0.73 +1.4 948,172 - -0.3 948,202 - +2.8 948,203 + +0.3 948,220 + +1.5 948,221 - -0.3 948,223 - -1.0 948,223 - -1.3 948,223 - -0.6 948,228 - +0.5 948,228 - -0.0 948,229 + +1.1
Or see this region's nucleotide sequence