Experiment: McCas_H2_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04270 and MMP_RS04275 are separated by 358 nucleotides MMP_RS04275 and MMP_RS04280 are separated by 16 nucleotides MMP_RS04280 and MMP_RS04285 are separated by 21 nucleotides
MMP_RS04270: MMP_RS04270 - coenzyme F420 hydrogenase subunit alpha, at 813,762 to 814,994
_RS04270
MMP_RS04275: MMP_RS04275 - hydrogenase iron-sulfur subunit, at 815,353 to 815,775
_RS04275
MMP_RS04280: MMP_RS04280 - coenzyme F420-non-reducing hydrogenase subunit gamma, at 815,792 to 816,694
_RS04280
MMP_RS04285: MMP_RS04285 - Ni/Fe hydrogenase subunit alpha, at 816,716 to 818,131
_RS04285
Position (kb) 815
816 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 814.568 kb on - strand, within MMP_RS04270 at 814.568 kb on - strand, within MMP_RS04270 at 814.618 kb on - strand, within MMP_RS04270 at 814.641 kb on - strand, within MMP_RS04270 at 814.659 kb on - strand, within MMP_RS04270 at 814.770 kb on - strand, within MMP_RS04270 at 814.890 kb on - strand at 814.894 kb on - strand at 814.895 kb on + strand at 815.283 kb on + strand at 815.407 kb on - strand, within MMP_RS04275 at 815.407 kb on - strand, within MMP_RS04275 at 815.422 kb on + strand, within MMP_RS04275 at 815.436 kb on - strand, within MMP_RS04275 at 815.446 kb on - strand, within MMP_RS04275 at 815.460 kb on + strand, within MMP_RS04275 at 815.465 kb on - strand, within MMP_RS04275 at 815.465 kb on - strand, within MMP_RS04275 at 815.510 kb on - strand, within MMP_RS04275 at 815.521 kb on + strand, within MMP_RS04275 at 815.621 kb on + strand, within MMP_RS04275 at 815.706 kb on - strand, within MMP_RS04275 at 815.770 kb on + strand at 815.778 kb on + strand at 815.778 kb on + strand at 815.781 kb on + strand at 815.781 kb on + strand at 815.781 kb on + strand at 815.781 kb on + strand at 815.807 kb on + strand at 815.807 kb on + strand at 815.841 kb on - strand at 815.841 kb on - strand at 815.842 kb on + strand at 815.856 kb on - strand at 815.856 kb on - strand at 816.082 kb on + strand, within MMP_RS04280 at 816.121 kb on - strand, within MMP_RS04280 at 816.268 kb on + strand, within MMP_RS04280 at 816.310 kb on - strand, within MMP_RS04280 at 816.449 kb on + strand, within MMP_RS04280 at 816.472 kb on + strand, within MMP_RS04280 at 816.472 kb on + strand, within MMP_RS04280 at 816.472 kb on + strand, within MMP_RS04280 at 816.496 kb on - strand, within MMP_RS04280 at 816.520 kb on - strand, within MMP_RS04280 at 816.542 kb on + strand, within MMP_RS04280 at 816.570 kb on + strand, within MMP_RS04280 at 816.692 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_H2_complete Antimycin A 0.01 mM remove 814,568 - MMP_RS04270 0.65 -2.8 814,568 - MMP_RS04270 0.65 +1.6 814,618 - MMP_RS04270 0.69 +1.4 814,641 - MMP_RS04270 0.71 -0.9 814,659 - MMP_RS04270 0.73 +0.4 814,770 - MMP_RS04270 0.82 +1.9 814,890 - +0.0 814,894 - -0.5 814,895 + +0.7 815,283 + +0.2 815,407 - MMP_RS04275 0.13 -0.9 815,407 - MMP_RS04275 0.13 +0.4 815,422 + MMP_RS04275 0.16 -1.3 815,436 - MMP_RS04275 0.20 -0.4 815,446 - MMP_RS04275 0.22 +0.3 815,460 + MMP_RS04275 0.25 +2.1 815,465 - MMP_RS04275 0.26 +1.1 815,465 - MMP_RS04275 0.26 -0.0 815,510 - MMP_RS04275 0.37 -0.7 815,521 + MMP_RS04275 0.40 -1.0 815,621 + MMP_RS04275 0.63 -0.3 815,706 - MMP_RS04275 0.83 -1.3 815,770 + -0.6 815,778 + +1.0 815,778 + +0.1 815,781 + -0.5 815,781 + +0.5 815,781 + +1.4 815,781 + -1.5 815,807 + -0.4 815,807 + +1.5 815,841 - -1.6 815,841 - -0.6 815,842 + +0.5 815,856 - +2.4 815,856 - +0.6 816,082 + MMP_RS04280 0.32 +1.5 816,121 - MMP_RS04280 0.36 -0.1 816,268 + MMP_RS04280 0.53 +0.2 816,310 - MMP_RS04280 0.57 +1.5 816,449 + MMP_RS04280 0.73 -0.4 816,472 + MMP_RS04280 0.75 +0.4 816,472 + MMP_RS04280 0.75 -0.7 816,472 + MMP_RS04280 0.75 -0.1 816,496 - MMP_RS04280 0.78 +0.4 816,520 - MMP_RS04280 0.81 +0.1 816,542 + MMP_RS04280 0.83 -0.0 816,570 + MMP_RS04280 0.86 -0.3 816,692 - +1.6
Or see this region's nucleotide sequence